Injectable bone repair hydrogel and preparation method thereof

A hydrogel and bone repair technology, applied in the field of medicine, can solve the problems of easy disintegration in contact with body fluids, temperature rising to 90°C, long curing time, etc., to promote growth and repair, mild reaction conditions, and improve mechanical properties Effect

Inactive Publication Date: 2017-01-11
中国人民解放军西部战区总医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are deficiencies in the injectable bone repair materials currently used clinically. For example, the temperature of methyl methacrylate (PMMA) bone cement rises to 90°C or higher due to the exothermic heat of on-site curing, which may cause necrosis of surrounding tissues. ; and its biocompatibility is poor, non-degradable, residual monomers and initiators are cytotoxic
Calcium phosphate cement (CPC) has long curing time, poor bonding performance, insufficient water resistance, easy disintegration when exposed to body fluids during the initial setting stage, and difficult mechanical properties to match normal bone. Once the uncured micro-nano powder enters the cardiovascular system, it will cause blood vessel blockage; calcium sulfate dissolves quickly, easily collapses in body fluids, and lacks osteoinductivity
In summary, traditional injectable bone repair materials still have certain defects and are not suitable for biomedical fields.

Method used

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  • Injectable bone repair hydrogel and preparation method thereof
  • Injectable bone repair hydrogel and preparation method thereof
  • Injectable bone repair hydrogel and preparation method thereof

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preparation example Construction

[0024] A preparation method of injectable bone repair hydrogel, the specific steps are as follows:

[0025] A. Polydopamine (PDA) modified calcium phosphorus particles

[0026] Disperse calcium-phosphorus particles in 0.5-5 mg / ml dopamine (DA) Tris-HCl solution to form a 0.25%-1% W / V solution, stir for 6-24 hours and then centrifuge to obtain PDA-modified calcium-phosphorus particles (PCP);

[0027] B. Fixed PCP

[0028] Add PDA-modified calcium phosphorus particles (PCP) to 5-20 wt% of degradable natural macromolecular phosphate buffer solution (PBS) with aldehyde groups, and fully mix, wherein the concentration of PCP is 5-20 wt%, to obtain solid A degradable natural polymer phosphate buffer solution with aldehyde groups loaded with PDA-modified calcium-phosphorus particles;

[0029] C. Preparation of hydrogel

[0030] Dissolve the degradable natural polymer with amino groups in phosphate buffer solution (PBS) to form a 2-20 wt% solution, and then mix it with the above-m...

Embodiment 1

[0037] A. PDA modified nano-hydroxyapatite

[0038] Disperse 2g of nano-hydroxyapatite (HA) (particle size 150-200nm) in 100ml Tris-HCl solution of dopamine (DA) with a concentration of 5mg / ml, stir for 12h and then centrifuge to obtain PDA-modified nano-hydroxyapatite Apatite (PHA);

[0039] B, preparation of oxidized sodium alginate (ADA)

[0040] Disperse 5.0g of sodium alginate in 25ml of ethanol to form suspension I, dissolve 3.25g of sodium periodate in 25ml of distillation to form solution II, then add solution II to suspension I, keep stirring for 6 hours before adding 2.5ml of ethylene glycol was used to stop the reaction for 2h, and the reaction mixture was added to 200ml of absolute ethanol which was stirred vigorously. After suction filtration, the product was fully dissolved in distilled water and put into a dialysis bag for dialysis for 3 days; finally, the white product was put into a watch glass and freeze-dried for 3 days to obtain oxidized sodium alginate; ...

Embodiment 2

[0047] The difference between this embodiment and embodiment 1 is:

[0048] C. Fixation of PHA

[0049] Add PHA to 20wt% ADA phosphate buffer solution (PBS), fully mix, wherein the concentration of PHA is 20wt%, obtain ADA-PHA solution;

[0050] The gel time of the hydrogel was determined to be about 5 min.

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Abstract

The invention discloses an injectable bone repair hydrogel and preparation method thereof. The hydrogel is prepared according to the following steps: carrying poly-dopamine (PDA) modified calcium phosphate particles on degradable natural polymers with aldehyde group in an immobilized way, and then mixing the degradable natural polymers with amino group and the degradable natural polymers carrying the PDA modified calcium phosphate particles in an immobilized way and having an aldehyde group to generate Schiff base reaction. The hydrogel disclosed by the invention is formed by the Schiff base reaction of natural polymers in physiological conditions, does not release heat violently, cannot cause damage of peripheral tissues in clinical application, and is applicable to clinical born repair materials.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to an injectable bone repair hydrogel and a preparation method thereof. Background technique [0002] Bone loses some of its bone mass due to osteoporosis, trauma, tumors, infections, and other diseases, creating large gaps called bony defects. The vast majority of bone defects are difficult to heal and eventually form nonunion. For a long time, the repair of bone defects mainly adopts autologous bone grafting or allogeneic bone grafting. Because autologous bone has both osteoinductive activity and osteoconduction, and carries osteogenic bone marrow cells, it is still regarded as a "Gold standard", but the source of autologous bone is limited, and bone extraction increases the suffering of patients; allogeneic bone has different degrees of immune rejection and has the potential risk of disease transmission. In recent years, artificial bone substitute materials made of various m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/58A61L27/52A61L27/50A61L27/22A61L27/20A61L27/18A61L27/12
CPCA61L27/12A61L27/18A61L27/20A61L27/222A61L27/50A61L27/52A61L27/58A61L2400/06A61L2430/02C08L89/06C08L5/04C08L79/02
Inventor 郑伟鲁雄刘宸吴娟甘东林刘达盛珺
Owner 中国人民解放军西部战区总医院
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