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Method for effectively extracting nutrient substances in seaweed

A technology for extracting seaweed and nutrients, applied in organic fertilizers, climate change adaptation, etc., can solve the problem that it is difficult to completely crack the cell wall of plants, and achieve the effect of improving the extraction rate

Inactive Publication Date: 2017-03-15
威海红印食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of sodium alginate by enzymatic extraction is not mature enough, and a single cellulase is often used for hydrolysis, but the plant cell wall consists of cellulase, hemicellulase, pectin, lignin and other components. The link between them is either mosaic or cohesion, so it is difficult to completely break the cell wall of the plant with a single enzyme, and the contents cannot be completely released

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Remove silt from the seaweed raw material, dry it at 60°C, accurately weigh 100g of seaweed after drying and crushing, add fresh drinking water with a raw material weight of 700ml, soak in a 50°C water bath for 2h; then adjust the pH value to 3 with HCl, add 0.3% cellulase composed of 45% endo-β-glucanase, 35% exo-β-glucanase, and 20% β-glucanase, hydrolyzed for 6 hours; then, add neutral protease 50% %, 0.2% protease composed of 50% papain, 0.2% pectinase composed of 60% pectin lyase and 40% pectin esterase, continue the enzymatic hydrolysis for 3 hours, stir continuously during the enzymatic hydrolysis process, centrifuge after the end of the enzymatic hydrolysis Separated to obtain pretreated kelp and enzymatic hydrolyzate.

[0027] Add 1200ml of 1% Na to the pretreated kelp 2 CO 3 Digest the solution in a water bath at 50°C for 2h, then add 4000ml of fresh drinking water at 50°C to dilute for 1h, and filter it through a 280-mesh silk screen after fully diluting; a...

Embodiment 2

[0031]Seaweed raw materials are removed from sediment, dried at 70°C, accurately weighed 100g of dried and crushed kelp, added fresh drinking water with a raw material weight of 800ml, soaked in a 60°C water bath for 2.5h; then adjusted the pH value to 6 with HCl, Add 0.5% cellulase consisting of 45% endo-β-glucanase, 35% exo-β-glucanase, and 20% β-glucosativa, and hydrolyze for 10 hours; then, add neutral protease 50%, 0.3% protease composed of 50% papain, 0.4% pectinase composed of 60% pectin lyase and 40% pectin esterase, continue enzymolysis for 5 hours, keep stirring during the enzymolysis process, Centrifugal separation to obtain pretreated kelp and enzymatic hydrolyzate.

[0032] Add 1,500ml of 3% Na2CO3 solution to the pretreated kelp, digest it in a 60°C water bath for 3 hours, then add 7,000ml of fresh drinking water at 60°C to dilute for 2 hours, and filter it through a 350-mesh silk screen after fully diluting; Decolorize 9ml of hydrogen peroxide at 60°C for 2h, t...

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PUM

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Abstract

The invention discloses a method for effectively extracting nutrient substances in seaweed. The method is characterized by including the steps of 1), pretreatment of raw materials; 2), water bath soaking; 3), fiber enzymolysis; 4), proteolysis; 5), salt water digestion; 6), diafiltration; 7), decoloration; 8), CaCl2 solution treatment; 9), ion exchange; 10), preparation of sodium alginate; 12), preparation of seaweed fertilizer. Compound enzyme is prepared through scientific matching of protease, pectinase and cellulose, the raw materials are pretreated, and extraction ratio of sodium alginate is favorably increased; meanwhile, fuel ethanol is prepared by the aid of kelp processing waste rich in cellulose; residual liquid waste and residual waste during processing are manufactured into seaweed organic fertilizer through concentration, spray-drying and granulation, high-valued comprehensive utilization of the seaweed is realized, and marine biological energy is developed.

Description

technical field [0001] The invention relates to the field of seaweed utilization, in particular to a method for efficiently extracting nutrients from seaweed. Background technique [0002] At present, the world is facing the huge pressure of energy shortage and environmental pollution, and the development of clean and renewable energy has become an important means to solve the crisis of petrochemical resources and energy. Bioethanol has been widely concerned as a clean fuel and gasoline additive. At present, the preparation of fuel ethanol in my country mainly focuses on terrestrial raw materials such as straw, bagasse, wood chips and cassava, but there are few studies on the preparation of bioethanol from marine biological resources, and the technical research on the development of seaweed biomass energy is relatively backward, and there is no Research results on the production of bioethanol from seaweed processing waste. [0003] The ocean area is huge, and there are rich...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/04C05F9/04
CPCC05F9/04C08B37/0003C08B37/0084Y02A40/20
Inventor 迟桂丽
Owner 威海红印食品有限公司
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