Phenyl borate compound and benzoyl peroxide detection kit containing the compound
A technology of benzoyl peroxide and phenyl borate, which is applied in the field of phenyl borate compounds and benzoyl peroxide detection kits, can solve sensitivity and accuracy interference, and is not suitable for biological sample detection, sample Deal with cumbersome problems, achieve high accuracy and sensitivity, easy large-scale production, and high sensitivity
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Embodiment 1
[0062] Embodiment 1: the preparation of formula (I) phenyl borate compound
[0063] (1-1) Preparation of compound (III)
[0064]
[0065] Compound (III) can be directly purchased or prepared according to the following method:
[0066] Add 30 mL of toluene to a three-neck flask equipped with a Dean-Stark trap, followed by adding 2-methylphenylboronic acid (0.21 g, 1.55 mmol), pinacol-H2 O, 0.22g, 1.86mmol), the resulting mixture was heated to reflux for two hours. After the reaction, the mixture was washed with water (30 mL×3) to remove unreacted pinacol and phenylboronic acid, and then the separated organic phase was evaporated under reduced pressure to remove the toluene solvent. The obtained crude product was dissolved in dichloromethane (50 mL), washed with water (30 mL) again, anhydrous sodium sulfate was added to the obtained organic phase for drying, and the dried solution was evaporated under reduced pressure to obtain 0.32 g of crude product.
[0067] Get the ac...
Embodiment 2
[0090] Embodiment 2: the spectral properties of fluorescent probe shown in formula (I) and different concentrations of benzoyl peroxide reaction
[0091] First prepare the phosphate buffered saline (PBS) of 10mM PH=7.4 containing ethanol, prepare the phosphate buffered saline solution (PBS) that phosphate concentration is 10mM, pH is 7.4, wherein the volume percent concentration of ethanol is For 10% PBS preparation using NaHPO 4 -KH 2 PO 4 system, so the phosphate concentration refers to NaHPO 4 and KH 2 PO 4 The total concentration, then, the prepared solution as the reagent stock solution a. The phenyl borate compound (I) prepared in Example 1 was formulated into a solution with a concentration of 1 mM as the reagent stock solution b, and the selected solvent was acetonitrile to obtain the reagent stock solution b. It has been verified that the type of organic solvent used to prepare the reagent stock solution b does not affect the detection effect, and the effect i...
Embodiment 3
[0101] Embodiment 3: the reaction situation (selectivity research) that contains the kit of fluorescent probe (I) and other ions
[0102] Dissolve reagent stock solution b at 1 mM concentration in reagent stock solution a (10 mM, pH 7.4) to prepare a 10 μM fluorescent probe solution, and add equal volumes of various common inorganic salts, biological media, active oxidation agents, etc. Compounds and typical oxides, such as 30μM calcium chloride, magnesium chloride, copper chloride, ferrous chloride, zinc sulfate, ferric chloride; 1.5mM vitamin B6, vitamin C, glucose, arginine, serine; 30μM Hypochlorite, Hydroxide, Singlet Oxygen, Nitric Oxide, Nitrite, Potassium Permanganate, Potassium Dichromate, Potassium Chlorate, Sodium Chlorate, Sodium Perchlorate, Potassium Iodate, Sodium Hypochlorite, Hydrogen Peroxide and 6 μM benzoyl peroxide (as a control). For interfering substances, the reagent stock solution a with a concentration of 10 mM was used as a solvent to obtain a set...
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