Preparation method of carbon nano-particles from mature vinegar

Abstract

The invention discloses a preparation method of carbon nano-particles from mature vinegar, in which carbon nano-particles are separated and purified and then collected through a macroporous resin adsorption method with the mature vinegar as a main raw material. The carbon nano-particles can be subjected to fluorescence resonance energy transfer with dopamine, wherein optical features of the carbonnano-particles are changed; under ultraviolet irradiation at 365 nm, blue fluorescence is gradually turned into green fluorescence. The color-adjustable carbon nano-particles can be directly appliedto living cell fluorescent imaging. The preparation method is simple and is low in cost. The carbon nano-particles are low in toxicity and have good biocompatibility. After reaction with the dopamine,water solubility of fluorescent carbon nano-particles is excellent, so that the carbon nano-particles can be used for living cell imaging as a fluorescent dye, and can be applied to the fields of fluorescence labeling and biology.

Description

technical field [0001] The invention relates to a preparation method of carbon nanoparticles from aged vinegar. Background technique [0002] Cells are the basic unit of organic form and function. Most of the synthesis and decomposition of substances are completed in cells, such as protein synthesis, glucose decomposition, etc. Therefore, an accurate and in-depth understanding of various chemical changes in cells It plays a very important role in the early diagnosis and treatment of some major diseases. However, the traditional cell fluorescent labeling substances have certain toxic side effects and will cause a certain degree of damage to the cells. Therefore, a non-toxic side effect, biocompatible Good fluorescent materials with good marking effect are particularly important. [0003] The preparation process of traditional organic fluorescent dyes is complicated, the fluorescence is unstable, easy to photobleach, and the general biocompatibility is poor, so its applicatio...

AI Technical Summary

Problems solved by technology

[0002] Cells are the basic unit of organic form and function. Most of the synthesis and decomposition of substances are completed in cells, such as protein synthesis, glucose decomposition, etc. Therefore, an accurate and in-depth understanding of various chemical changes in cells It plays a very important role in the early diagnosis and treatment of some major diseases. However, the traditional cell fluorescent labeling substances have certain toxic side effects and will cause a certain degree of damage to the cells. Therefore, a non-toxic side effect, biocompatible Fluorescent materials with good marking effect are particularly important

[0003] The preparation process of traditional organic fluorescent dyes is complicated, the fluorescence is unstable, easy to photobleach, and the general biocompatibility is poor, so its application is limited to a certain extent. Compared with traditional organic fluorescent dyes, fluorescent nanomaterials overcome These disadvantages are overcome, and it is widely used in the fields of biomarkers, biomedicine, etc. Among them, quantum dots are the most widely used fluorescent nanomaterials. Quantum dots, as a type of semiconductor carbon nanoparticles developed in recent years, have unique optical properties and electrical properties. However, common quantum dots (such as CdSe) contain toxic elements, which will harm the environment and affect the activity of cells or biomacromolecules. Therefore, it is urgent to find a fluorescent probe with no toxic side effects and good biocompatibility. Carbon nanoparticles are a new star in the family of fluorescent nanomaterials, which have unique advantages such as chemical stability, low toxicity, and excellent biocompatibility, and can be used as ideal cell labeling materials.

[0004] The methods commonly used to prepare carbon nanoparticles are divided into two methods: top-down and bottom-up. Nanoparticles, including: electrolysis, acid etching, laser etching, and high-temperature pyrolysis; and the bottom-up method refers to the synthesis and agglomeration of small molecular carbon materials into larger molecular weight carbon materials through certain chemical means. Nanoparticles, including: chemical synthesis method, hydrothermal method, solvothermal method, etc. However, the conditions of the above preparation methods are relatively strict. Not only does it take a long time, but also requires high temperature. The process is very complicated. Food contains a lot of carbohydrates, and carbon nanoparticles will be formed during thermal processing. Mature vinegar, as a traditional condiment in China, has been eaten by people for thousands of years. The carbon nanoparticles prepared from it have no toxic side effects and have Good biocompatibility, therefore, has broad prospects in biological applications such as cell imaging

Images