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A biomimetic tissue engineering scaffold containing micropores and nanofiber composite structure and preparation method thereof

A technology for tissue engineering scaffolds and nanofibers, which can be used in fiber processing, tissue regeneration, and pharmaceutical formulations. It can solve the problems of small pores, difficult cells, and poor mechanical properties of scaffolds, and achieve the effect of promoting cell adhesion.

Active Publication Date: 2020-01-14
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the electrospinning process is that the prepared scaffold has small pores, it is difficult for cells to grow into the scaffold, and the general mechanical properties are poor.
It can be seen that none of the above existing methods can prepare biomimetic tissue engineering scaffolds with both micropores and nanofiber networks.

Method used

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  • A biomimetic tissue engineering scaffold containing micropores and nanofiber composite structure and preparation method thereof
  • A biomimetic tissue engineering scaffold containing micropores and nanofiber composite structure and preparation method thereof
  • A biomimetic tissue engineering scaffold containing micropores and nanofiber composite structure and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Preparation of biomimetic tissue engineering scaffolds with micropores and nanofiber networks

[0050] Dissolve gelatin (15k~25kDa) in PBS buffer solution to prepare gelatin solution with a concentration of 0.01g / mL; dissolve sodium alginate in PBS buffer solution to prepare alginic acid with a concentration of 0.01g / mL Sodium solution; the above two solutions are prepared into a uniform, bubble-free, precipitation-free mixed solution, that is, a gelatin-sodium alginate mixed solution, the mass ratio of gelatin to sodium alginate is 1:1, and the concentration of gelatin is 0.005g / mL, the concentration of sodium alginate was 0.005g / mL.

[0051] Type I rat tail (Collagen, sigma) collagen was dissolved in 1% acetic acid solution to prepare a collagen solution with a concentration of 0.08 g / mL, cross-linked with 0.5 wt% genipin solution, and then dissolved in hexafluoroiso In propanol (HFIP), collagen fibers (265±83 nm in diameter) were prepared by electrospinn...

Embodiment 2

[0054] Example 2: Preparation of biomimetic tissue engineering scaffolds with micropores and nanofiber networks

[0055] Rat tail collagen type I (SIGMA company, product number C7661) was dissolved in 1.0wt% acetic acid solution to prepare a collagen solution with a mass fraction of 0.01 g / mL; chitosan was dissolved in 1.0wt% acetic acid solution to prepare A chitosan solution with a mass fraction of 0.01 g / mL was prepared; the above two solutions were prepared into a uniform, bubble-free, and precipitation-free mixed solution, that is, a collagen-chitosan solution, and the mass ratio of collagen to chitosan was 1 : 1, the concentration of collagen is 0.005g / mL, and the concentration of chitosan is 0.005g / mL.

[0056] Polycaprolactone (PCL) was dissolved in hexafluoroisopropanol (HFIP) to prepare a PCL solution of 0.1 g / mL, and an electrospinning device was used to prepare PCL fibers (2.0 ± 0.5 μm in diameter), in which the syringe extruded The speed was 0.8 ml / h, the voltage...

Embodiment 3

[0059] Example 3: Preparation of biomimetic tissue engineering scaffolds with micropores and nanofiber networks

[0060] Dissolve silk fibroin (silk fiber) in 1.0wt% acetic acid solution to prepare a silk fibroin solution with a mass fraction of 0.005g / mL; dissolve chitosan in 1.0wt% acetic acid solution to prepare a mass fraction of 0.015g / mL chitosan solution; the above two solutions were prepared into a uniform, bubble-free, and precipitation-free mixed solution, namely silk fibroin-chitosan solution, and the mass ratio of silk fibroin to chitosan was 3 : 1, the concentration of silk fibroin is 0.005g / mL, and the concentration of chitosan is 0.015g / mL.

[0061] Dissolve poly-D,L-lactide (PDLLA) in chloroform to prepare 0.15g / mL PDLLA solution, and use electrospinning equipment to prepare PDLLA fibers (5.0±2.5μm in diameter), in which the syringe extrusion speed It was 1.6ml / h, the voltage was 20kV, and the collecting device was about 15cm away from the needle tip. The col...

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Abstract

The invention discloses a bionic tissue engineering scaffold containing a micropore and nanofiber composite structure and a preparation method thereof. The bionic tissue engineering scaffold comprisesmicropores and a nanofiber network structure. The micropores have diameters of 20-200 micrometers, the diameter of the nanofiber network structure is 10-2000nm and the length of the nanofiber networkstructure is 1-100 micrometers. The bionic tissue engineering scaffold fully simulates the structural features of a natural extracellular matrix, the micropores provide conditions for cell growth into the scaffold and high porosity is conducive to permeation and diffusion of oxygen and nutrients. The network structure of the nanometer fiber network bionic extracellular matrix can promote cell adhesion, growth, proliferation, differentiation and migration. The preparation method well solves the problems of the existing traditional scaffold preparation method and has the potential of becoming anovel tissue engineering scaffold preparation technology.

Description

technical field [0001] The invention relates to a bionic tissue engineering scaffold containing micropores and nanofiber composite structures and a preparation method thereof, belonging to the technical fields of tissue engineering and biomanufacturing. Background technique [0002] Tissue Engineering and Regenerative Medicine is an emerging interdisciplinary science that repairs and replaces damaged tissues and organs by constructing artificial tissues with certain structures and functions in vitro. At present, tissue engineering products have been applied in the fields of skin, bone / cartilage, bladder, blood vessels, liver, nerves, etc., providing new possibilities for human treatment of various tissue and organ injuries. The classic strategy of tissue engineering consists of three elements: cells, scaffolds and growth factors. Among them, scaffolds provide structural support for cells through biomimetic extracellular matrix (ECM) and promote cell adhesion, growth, prolife...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/56A61L27/50D01D5/00D04H1/728
CPCA61L27/26A61L27/50A61L27/56A61L2400/12A61L2430/40D01D5/003D01D5/0076D01D5/0092D04H1/728C08L5/04C08L5/08C08L67/04C08L89/00
Inventor 孙伟方永聪张婷莉莉安娜.利维拉尼奥尔多·博卡奇尼
Owner TSINGHUA UNIV
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