BSA (Bovine Serum Albumin)-gadolinium ion complex wrapped hollow golden nano shell and preparation method

An ionic complex and gold nanoshell technology, which is applied in the field of hollow gold nanoshells and preparation, can solve problems such as no hollow gold nanoshells reported yet, and achieves stable and reliable preparation methods, readily available raw materials, and strong reaction controllability. Effect

Active Publication Date: 2018-03-20
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on hollow gold nanoshells loaded with BSA-gadolinium ion complex

Method used

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  • BSA (Bovine Serum Albumin)-gadolinium ion complex wrapped hollow golden nano shell and preparation method
  • BSA (Bovine Serum Albumin)-gadolinium ion complex wrapped hollow golden nano shell and preparation method
  • BSA (Bovine Serum Albumin)-gadolinium ion complex wrapped hollow golden nano shell and preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation method of the hollow gold nanoshell loaded with BSA-gadolinium ion complex comprises the following steps:

[0045] (1) Mesoporous silica nanoparticles (mSiO 2 ) preparation:

[0046] A. Take 0.1g of the template agent cetyltrimethylammonium bromide (CTAB) and add it to 50mL of deionized water, heat to 80°C, adjust the pH to 9, add 320μL of tetraethyl orthosilicate (TEOS) and 80μL of N-(3-trimethoxysilylethyl)ethylenediamine (APTES), add 3mL ethyl acetate, react for 3 hours, centrifuge, and wash the precipitate 4 times with absolute ethanol;

[0047] B. Add 50 mL of absolute ethanol to the precipitate obtained in step A, heat at 60° C. to remove the template agent hexadecyltrimethylammonium bromide, centrifuge, and wash the precipitate 4 times with absolute ethanol;

[0048] C. Repeat step B 4 times to obtain mesoporous silica nanoparticles (mSiO 2 );

[0049] (2) Aminated mesoporous silica nanoparticles (NH 2 -mSiO 2 ) preparation:

[0050] Disper...

Embodiment 2

[0064] Test the photothermal heating curve of Au@BSA-Gd:

[0065] Take phosphate buffer solution with pH=7.4, and take 0.5ml of Au@BSA-Gd aqueous dispersion with different concentrations (17.5, 35, 70 and 140μg / mL) into EP tubes, and use 808nm, 1.5W / cm 2 The power laser was irradiated for 5 minutes, and the temperature change within 0-5 minutes was recorded using a temperature monitor equipped with a thermocouple microprobe (φ=0.5mm). Such as Figure 4 Shown is the photothermal heating curve of Au@BSA-Gd. It can be seen that Au@BSA-Gd can rapidly heat up under light to achieve the purpose of photothermal therapy, and the heating effect is enhanced as the concentration increases.

Embodiment 3

[0067] The survival rate of 4T1 cells in different groups (phosphate buffer group with pH=7.4, laser group, Au@BSA-Gd group and Au@BSA-Gd+laser group) tested by MTT method: 4T1 cells in logarithmic growth phase were taken as 5×10 4 Each well was inoculated in a 96-well plate and divided into four groups: blank phosphate buffer (PBS) group with a pH of 7.4, laser (NIR) group, Au@BSA-Gd group and Au@BSA-Gd+NIR group. After the cells grew for 24 hours, the original cell culture medium was aspirated in each group, 100 μL of 4T1 cell culture medium containing 10 μL of PBS (pH=7.4) was added to the phosphate buffer (PBS) group, and 100 μL of 4T1 cell culture medium was added to the laser (NIR) group. , Au@BSA-Gd group and Au@BSA-Gd+NIR group were added with 100 μL of 4T1 cell culture medium containing Au@BSA-Gd at a concentration of 100 μg / mL. After each group was incubated for 6 hours, the laser group and Au@BSA-Gd+NIR were given 808nm, 1.5W / cm 2 Power laser irradiation for 5 min...

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Abstract

The invention discloses a BSA (Bovine Serum Albumin)-gadolinium ion complex wrapped hollow golden nano shell and a preparation method. The preparation method comprises the following steps: (1) preparing mesoporous silica nanoparticles; (2) preparing aminated mesoporous silica nanoparticles; (3) preparing golden shell wrapped mesoporous silica nanoparticles; (4) preparing a hollow golden nano shell; (5) preparing a carboxyl modified hollow golden nano shell; (6) preparing a BSA-gadolinium ion complex; and (7) preparing the BSA-gadolinium ion complex wrapped hollow golden nano shell. The BSA-gadolinium ion complex wrapped hollow golden nano shell disclosed by the invention has a porous surface and an internal hollow structure, can be directly applied to diagnosis and treatment on cancer, canbe also used as a medicine carrier to prepare a cancer diagnosis medicine or an anti-cancer medicine, has a three-mode imaging (CT/PA/MR (Computed Tomography/Photoacoustic/Magnetic Resonance)) medicated photo-thermal treatment function, is capable of achieving visible monitoring on cancer, and is stable in property and good in dispersibility and biocompatibility.

Description

technical field [0001] The invention belongs to the field of nano-medicines, and in particular relates to a hollow gold nanoshell loaded with a BSA-gadolinium ion complex, a preparation method and an application. Background technique [0002] Cancer is one of the malignant diseases that threaten human health in the 21st century. In the past few decades, although human beings have made some achievements in the understanding and overcoming of cancer, the effective treatment of cancer still faces great challenges. Near-infrared light-induced photothermal therapy is an emerging non-invasive minimally invasive treatment technology for tumors in recent years. It mainly uses nanoparticles with photothermal conversion effect to convert near-infrared light energy into local heat energy to kill cancer cells, thereby greatly reducing cancer cells. Damage to normal tissue cells, so it has broad application prospects in cancer treatment. In addition, tumor diagnosis is the premise of i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/04A61K49/14A61K49/18A61K49/22A61K41/00A61P35/00
CPCA61K41/0052A61K49/0002A61K49/04A61K49/143A61K49/1824A61K49/221A61K49/225
Inventor 李楠游青孙琪王金平刘丽程玉王怡丹宋宜霖王思宇
Owner TIANJIN UNIV
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