Biosynthesis method of L-carnitine

Abstract

The invention discloses a biosynthesis method of L-carnitine. The biosynthesis method comprises: 1) dissolving a substrate ethyl 4-chloroacetoacetate in a MOPS buffer solution, adding ketoreductase-containing engineering bacteria whole cells, coenzyme, butylene glycol, a surfactant and an additive, and carrying out a reaction to generate (R)-4-chloro-3-hydroxy-butyric acid ethyl ester, wherein thepH value is controlled at 6.5-7.5; and 2) dissolving sodium hydroxide in a trimethylamine aqueous solution with a mass ratio of 25% to form a mixed solution, slowly adding the mixed solution to the (R)-4-chloro-3-hydroxy-butyric acid ethyl ester generated in the step 1) at a temperature of -5-5 DEG C in a dropwise manner, carrying out a reaction for 20-30 h at a temperature of -5-5 DEG C, stopping the reaction, adjusting the pH value to 5-7, and purifying to obtain L-carnitine. According to the present invention, the biosynthesis method has characteristics of strong stereoselectivity, mild reaction conditions, low cost, low pollution, high efficiency, high yield and high optical purity of the product.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a biosynthesis method of L-carnitine. Background technique [0002] L-carnitine is a kind of amino acid that promotes the conversion of fat into energy. Red meat is the main source of L-carnitine, which has no toxic and side effects on the human body. Different types of daily diets already contain 5-100 mg of L-carnitine, but the average person can only take in 50 mg a day from meals, and vegetarians take in even less. The main physiological function of L-carnitine is to promote the conversion of fat into energy. Taking L-carnitine can reduce body fat and weight without reducing water and muscle. In 2003, L-carnitine was recognized as The safest weight loss nutritional supplement without side effects. In recent years, L-carnitine has been widely used in the fields of nutrition and health care, weight loss and bodybuilding, improving athletic ability, fatigue recovery, food add...

AI Technical Summary

Problems solved by technology

The shortcoming that the preparation method of above-mentioned L-carnitine exists is: one, splitting method, no matter be chemical splitting or enzymatic splitting, research and development cycle is all longer, and splitting process route is more complicated, and the screening workload of enzyme is also relatively long. Larger, and most of them need to use more toxic cyanide as raw material

2. The asymmetric chemical synthesis of L-carnitine, the reaction process produces a large amount of sodium chloride. At present, the electrodialysis desalination process is widely used to remove sodium chloride, but the loss of L-carnitine in the desalination process is as high as 25 %, in addition, during the operation of the electrodialysis desalination process, the cathode and the membrane are easy to scale, thus affecting the quality of the effluent water and shortening the use time of the instrument; Washing, regular dismantling and washing and other complicated maintenance measures

In addition, the power consumption and water consumption of electrodialysis desalination treatment technology are very high, and the turbidity requirements for influent water are also very strict.