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Method for preparing nine types of natto kinase through millimeter wave culture medium

A medium preparation, nattokinase technology, applied in the biological field, can solve the problems of changes, nattokinase products cannot effectively play the role of nutrition and health care, etc., and achieve the effects of improving conversion rate, increasing yield and activity value, and promoting metabolism

Inactive Publication Date: 2018-12-21
江苏十亿康健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current domestic nattokinase products correspond to people with different physiques, and they all adopt a unified production formula, which cannot change with the change of people's physique, resulting in the inability of nattokinase products to effectively play the role of nutrition and health care

Method used

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  • Method for preparing nine types of natto kinase through millimeter wave culture medium
  • Method for preparing nine types of natto kinase through millimeter wave culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing nine-quality nattokinase from a millimeter-wave medium, comprising the following steps:

[0027] (1) In a closed environment, different varieties of natto were placed in different Erlenmeyer flasks, and sterile water was added to 30mL, and the birefringent photonic crystal fiber microwave millimeter wave generator extracted the frequency through the millimeter waveguide to be 53~ 60GHz millimeter wave, the waveguide mouth is continuously aligned with natto, the Erlenmeyer flask is placed in a 37°C shaker and shaken for 30min, taken out, boiled in a water bath for 5min, cooled, diluted and coated with LB solid medium, cultivated overnight at 37°C, Pick a single colony on the cultured LB plate medium and put it on the casein primary screening plate, cultivate overnight at 37°C, select the one with the larger ratio of the diameter of the transparent circle to the diameter of the colony and transfer it to the LB liquid medium to obtain the Bacillus nat...

Embodiment 2

[0035] Same as the steps in Example 1, the difference is:

[0036] In step (2), each component is weighed according to the following mass components: kidney bean powder 12-30g / L, red bean powder 9-15g / L, bitter melon powder 2-5g / L, tomato powder 2-5g / L, glucose 30-40g / L and sodium chloride 0.05-4g / L, and marked as group B (yin-deficiency constitution) millimeter-wave medium for producing nattokinase. Table 1 shows the nattokinase activity values ​​of group B (yin-deficiency constitution).

Embodiment 3

[0038] Same as the steps in Example 1, the difference is:

[0039] In step (2), weigh each component according to the following mass components: kidney bean powder 12-30g / L, glutinous rice flour 1-3g / L, ginkgo powder 1-3g / L, sweet potato powder 1-3g / L, glucose 30 ~ 40g / L and sodium chloride 0.05 ~ 4g / L, and marked as group C (qi deficiency constitution) millimeter wave medium for producing nattokinase. See Table 1 for the nattokinase activity values ​​of group C (qi-deficiency constitution).

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Abstract

The invention discloses a method for preparing nine types of natto kinase through a millimeter wave culture medium. The method belongs the technical field of biology and the problems in the prior artthat the activity of microorganism cells is low in a natto kinase fermentation process, the metabolism yield of the natto kinase is low, the production stability is poor and requirements of differenthuman bodies cannot be met. The method disclosed by the invention comprises the following steps: preparing a bacillus strain through millimeter wave irradiation, preparing nine types of culture mediumthrough the millimeter wave irradiation and fermenting after fermenting seeds; drying a fermentation solution. According to the method disclosed by the invention, the millimeter wave irradiation culture medium with different formulas are prepared according to nine types of different body constitutions; millimeter waves can rapidly and effectively act on cells in microorganisms; the biological transformation efficiency of the culture medium, the yield of the natto kinase and the activity of the natto kinase can be obviously improved; nine types of formulas can meet nutrient and health requirements on nine types of people with different body constitutions; a whole fermentation process is carried out in a millimeter wave environment with specific frequency and the content and activity of serinase are improved by utilizing a non-thermal effect of the millimeter waves.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing nine-quality nattokinase from a millimeter-wave medium. Background technique [0002] Thrombotic disease is a high incidence of middle-aged and elderly people. It is the second most serious disease after cancer and has a high threat of death. At present, thrombolysis is an important means of treatment for this type of disease. Nattokinase is a subtilisin protease, which is a serine protease produced by Bacillus subtilis natto during the fermentation process of natto. It has the functions of dissolving thrombus, reducing blood viscosity, improving blood circulation, softening and increasing blood vessel elasticity. Nattokinase stimulates vascular endothelial cells to produce tissue plasminogen activator t-PA, t-PA activates plasmin into plasmin, dissolves fibrin, and directly dissolves thrombus; Activated as urokinase, urokinase and t-PA activate pl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/56C12N1/20C12N13/00C12R1/07
CPCC12N1/20C12N9/54C12N13/00C12Y304/21062
Inventor 陆晓
Owner 江苏十亿康健康产业有限公司
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