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Preparation method and application of chicken manure anaerobic fermentation high load operation regulation fungicide

A technology of anammox bacteria and chicken manure, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as time-consuming and labor-consuming, system instability and acidification, and affecting system stability

Inactive Publication Date: 2019-02-12
浙江中科筑原生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the digestive tract of the chicken is short, so the feed stays in the digestive tract for a short time, and the digestion and absorption efficiency of the feed by the chicken is very low. The chicken manure is rich in organic matter, especially the crude protein. The characteristics of the chicken manure lead to During the anaerobic fermentation process of chicken manure, the COD and ammonia nitrogen content are high, which leads to the problem of ammonia inhibition and affects the stability of the system
In addition, high-load operation in the anaerobic digestion process tends to accumulate a large amount of volatile fatty acids (VFA), which can easily cause system instability and acidification
[0003] The anaerobic digestion system with chicken manure as raw material is prone to high ammonia nitrogen and high volatile fatty acids, resulting in double inhibition and poor system stability
The addition and adjustment of acid and alkali substances will affect the efficiency of the fermentation system in the later stage. It is time-consuming and labor-intensive to start the re-inoculation after discharging or large-scale re-inoculation. It also needs to be cultivated and acclimated to produce gas normally, which will cause great losses to the project.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of liquid compound biological preparation

[0051] Select 56L of inoculated sludge mainly using chicken manure as raw material in a 70L anaerobic fermentation tank. In order to improve the activity of the sludge, add methanogenic medium and a small amount of chicken manure in the early stage of domestication. During the period, the methane content is mainly used as the main monitoring index. When the methane production content of the system reaches more than 60%, it will be maintained for a period of time, and the organic matter in the biogas fermentation system will be exhausted.

[0052] Get 1L of the above-mentioned chicken manure inoculum, and prepare 1L of medium, which contains 5.0g of glucose, 0.8g of dipotassium hydrogen phosphate, 1.0g of magnesium sulfate, 0.5g of anhydrous calcium chloride, 8g of yeast powder, 10mL of the above trace elements and 2mL of vitamins, mix the inoculum with the culture medium, adjust the pH to 7.3, and blow ou...

Embodiment 2

[0053] Example 2 Preparation of solid composite biological preparation

[0054] The chicken manure was sterilized and dried, and mixed with the liquid biological agent obtained in Example 1 at a mass ratio of 1:1 under a high-purity nitrogen atmosphere, stirred evenly, and dried under the above-mentioned anaerobic conditions at a constant temperature of 37°C to the water content to 8%, add 5.0g / kg of glucose, 0.8g / kg of dipotassium hydrogen phosphate, 1.0g / kg of magnesium sulfate, 0.5g / kg of calcium chloride, 8.0g / kg of yeast powder, 7.0 g / kg of ammonium sulfate, 12.0g / kg of sodium acetate, 8.0g / kg of sodium propionate and 7.0g / kg of sodium butyrate are solid biocomposite preparations for biogas fermentation systems that use chicken manure as raw material. Store at 4°C under anaerobic conditions.

Embodiment 3

[0055] Example 3 Effect test of liquid compound biological preparation

[0056] Acidified biogas fermentation system with chicken manure as raw material, pH 6.9, CH 4 Content 35%, ammonia nitrogen content: 7000mg / L, acetic acid content: 12000mg / L, propionic acid content: 8000mg / L, butyric acid content: 7000mg / L. Set up seven groups of a, b, c, d, e, f, g, and h as control batch fermentation, take the above-mentioned acidified biogas fermentation liquid in a, b, c, d, e, and f; group g uses cow dung The raw material is high ammonia nitrogen and acidified biogas fermentation broth, pH value is 7.1, CH 4 content of 34%, ammonia nitrogen content of 7000mg / L, acetic acid content of 12000mg / L, propionic acid content of 8000mg / L, butyric acid content of 7000mg / L; group h uses pig manure as raw material for high ammonia nitrogen and acidified biogas fermentation solution, pH 6.9, CH 4 Content 35%, ammonia nitrogen content: 7000mg / L, acetic acid content: 15000mg / L, propionic acid: 8...

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PUM

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Abstract

The invention discloses a preparation method and application of a chicken manure anaerobic fermentation high load operation regulation fungicide. Liquid and solid compound biological preparations areprovided by aiming at a high ammonium nitrogen acidified biogas fermentation system; the high ammonium nitrogen acidified system with the gas yield being reduced or stopped can resume normal gas production; no discharging or large-scale refueling is needed; continuous and stable gas production of the biogas fermentation system can be guaranteed. In addition, the invention further provides a formula and the preparation method of the compound biological preparation aiming at resuming gas production of the high ammonium nitrogen acidified biogas fermentation system taking chicken manure as the raw material. The liquid compound biological preparation and the solid compound biological preparation can be prepared according to the method.

Description

technical field [0001] The invention relates to waste biological fermentation technology, in particular to a preparation method and application of a compound biological preparation for medium-temperature biogas fermentation of chicken manure waste with high ammonia nitrogen and high content of volatile fatty acids, and belongs to the technical field of biological fermentation. Background technique [0002] A large amount of malodorous substances will be produced in the process of poultry breeding and manure storage, which will cause serious harm to the health of humans and animals. Anaerobic digestion technology is an effective way to treat manure and convert the biomass of manure into biogas energy. Among them, the digestive tract of the chicken is short, so the feed stays in the digestive tract for a short time, and the digestion and absorption efficiency of the feed by the chicken is very low. Chicken manure is rich in organic matter, especially crude protein. The charact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F11/04C12R1/01C12R1/72C12R1/07C12R1/38C12R1/05C12R1/145
CPCC12N1/16C12N1/20C02F11/04Y02E50/30
Inventor 郑涛周政忠孙娟陈圣泓苏毅张亚兵
Owner 浙江中科筑原生态科技有限公司
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