Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus helveticus with cholesterol reducing capacity

A technology for lowering cholesterol and Lactobacillus, applied in the field of Lactobacillus helveticus, can solve the problem of low degradation rate, achieve the effect of reducing fat deposition, inhibiting pathogen infection, and good tolerance

Inactive Publication Date: 2019-03-12
甘肃普诺贝康生物科技有限责任公司
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there are reports of isolating cholesterol-lowering lactic acid bacteria from other fermented foods, but the degradation rate is not high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus helveticus with cholesterol reducing capacity
  • Lactobacillus helveticus with cholesterol reducing capacity
  • Lactobacillus helveticus with cholesterol reducing capacity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The used pathogenic bacterial strain of embodiment 1 bacterial strain screening culture medium and bacteriostasis test

[0028] 1. Relevant medium formula

[0029] MRS medium is a basic medium for cultivating lactic acid bacteria, M17 medium and SL medium are both mediums for isolating lactic acid bacteria, and SL medium is a medium for isolating acidophilic lactic acid bacteria.

[0030] Lactic acid bacteria medium MRS: peptone 10g, beef extract 10g, yeast extract 5g, diammonium hydrogen citrate 2g, glucose 20g, Tween-80 1mL, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, sulfate Manganese 0.25g, agar 15g, distilled water 1000mL, pH 6.2-6.6. Preparation method: add all ingredients except agar into water and heat to dissolve, adjust the pH to 6.2-6.4, add agar, sterilize at 121°C for 15 minutes, and pour it onto a plate while it is hot.

[0031] M17 medium: Accurately weigh 5.0g of phytopeptone, 5.0g of yeast extract, 5.0g of polypepton...

Embodiment 2

[0038] Example 2: Isolation and identification of lactic acid bacteria from traditional fermented yak yogurt on the Qinghai-Tibet Plateau

[0039] 1. Sample source

[0040] It comes from 720 traditional fermented yak yoghurts collected from 6 regions of Qinghai-Tibet Plateau (Naqu, Maqu, Hongyuan, Yushu, Tianzhu and Ruoergai).

[0041] 2. Lactic acid bacteria isolated from traditional yak dairy products

[0042] Take 1mL of fermented yak milk product and dilute it with sterilized saline at a ratio of 1:10, which is 10 -1 dilution. Then draw 1mL of the above diluent, and then dilute it 10 times, which is 10 -2 dilution, and so on. Pick 10 -5 、10 -6 、10 -7 For three dilutions, draw 100 μL of the dilutions and spread them evenly on the MRS, M17, KFS and SL (adjust pH 5.4) plate culture medium respectively. Cultivate for 48 hours, pick characteristic colonies, and further purify to obtain pure isolated lactic acid bacteria strains.

[0043] 3. Identification of strains

...

Embodiment 3

[0050] Embodiment 3: the screening of high cholesterol-lowering lactic acid bacteria

[0051] 1. Preparation of water-soluble cholesterol solution and lactic acid bacteria

[0052] Prepare the water-soluble cholesterol solution, and use a filter membrane to filter and sterilize it in an ultra-clean workbench. After the lactic acid bacteria sample is activated for 3-4 generations (the last activation culture time is 12-16h), adjust the number of bacteria to 1.0×109 cfu / mL, and inoculate the cow with 0.3% In the MRS-Thio medium of bile powder, the amount of water-soluble cholesterol added was 100 μL / mL. The blank control was the MRS-Thio medium containing water-soluble cholesterol without adding bacterial solution. Sampling 1mL, 10000rpm / min, 2min, take 0.5mL supernatant, and use the o-phthalaldehyde method to measure the cholesterol content in the culture solution after inoculation. The rest of the fermentation broth was cultured statically at 37°C for 24h.

[0053] 2. Dete...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses lactobacillus helveticus with cholesterol reducing capacity. The preservation number is CGMCC15778. The lactobacillus helveticus has the cholesterol reducing capacity is separated from traditional fermented yak yogurt from Qinghai-Tibet Plateau, can significantly reduce the serum total cholesterol, triglyceride and low-density lipoprotein, has high antibacterial capacity, has given inhibition capacity for four pathogenic bacteria, i.e. escherichia coli, staphylococcus aureus, listeriosis and salmonella typhimurium, and can be used for preparing fermentation food as wellas preparing healthcare products and drugs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of development and application of functional lactic acid bacteria, in particular to a strain of Lactobacillus helveticus capable of lowering cholesterol. Background technique [0002] Excess blood cholesterol is a major factor in cardiovascular disease. It has been reported that a 1% reduction in serum cholesterol can reduce cardiovascular disease by 2% to 3% (Manson et al, 1992). Treatment of hyperlipidemia by drug therapy is not only expensive but also has side effects on human health. For this reason, exploring the supplementation of a substance that can lower serum cholesterol in the diet has aroused great interest of scientists. In recent years, lactic acid bacteria have received widespread attention as a potential cholesterol-lowering dairy additive (Chandan et al., 1999; Nicole et al., 2000). A large number of studies have shown that lactic acid bacteria can effec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N1/02A23C9/123A61K35/747A61P3/06C12R1/225
CPCA61P3/06A23C9/1234A23L33/135C12N1/02A23V2002/00A61K2035/115C12R2001/225C12N1/205A23V2400/147A23V2200/3262A23V2200/30A23V2200/324Y02A50/30
Inventor 郭旭生丁武蓉王丽娜柯文灿
Owner 甘肃普诺贝康生物科技有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com