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Photoelectric immunosensor based on water-soluble Zn-Mg-Te quantum dot/titanium dioxide nanorod composite material

A zn-mg-te, titanium dioxide technology, applied in the direction of titanium dioxide, selenium/tellurium compounds, material electrochemical variables, etc., can solve the problem of low photoelectric conversion efficiency, to maintain antibody biological activity, strong fluorescence intensity and stability, The effect of good application prospects

Inactive Publication Date: 2019-05-03
DONGGUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Quantum dots are an important semiconductor optoelectronic material widely used in photoelectrochemical sensing research, but the photoelectric conversion efficiency of a single quantum dot material is low due to the recombination of internal electron-hole pairs; therefore, it is necessary to find a suitable method to effectively It is very important to improve the photoelectric conversion efficiency of photoelectric materials; with the advancement of new material technology, the importance of nanomaterials has become increasingly prominent, which has injected new power into photoelectrochemical sensors; especially nanocomposite materials, which have more High photoelectric conversion efficiency; in recent years, research on nanocomposites has received widespread attention

Method used

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  • Photoelectric immunosensor based on water-soluble Zn-Mg-Te quantum dot/titanium dioxide nanorod composite material
  • Photoelectric immunosensor based on water-soluble Zn-Mg-Te quantum dot/titanium dioxide nanorod composite material
  • Photoelectric immunosensor based on water-soluble Zn-Mg-Te quantum dot/titanium dioxide nanorod composite material

Examples

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Embodiment 1

[0046] A preparation method of water-soluble Zn-Mg-Te quantum dots, comprising steps as follows:

[0047] Preparation of NaHTe solution: under the protection of nitrogen, add 80mg NaBH4 and 10ml water into a 25ml three-necked flask in turn, react in an ice bath, add 127mg tellurium powder, and react for 4 hours. After the black tellurium powder reacts completely, a colorless and transparent NaHTe solution is obtained, which is set aside ;

[0048] Scheme 1: Under the protection of nitrogen, add 60mL 0.002mol / L ZnCl to the three-neck round bottom flask successively 2 solution, 20mL 0.016mol / L N-acetyl-L-cysteine ​​solution and 20mL double distilled water, mix well to obtain a mixed solution; use 1mol / L NaOH solution to adjust the pH value of the mixed solution to 11.5; add 0.5mL 0.01mol / L MgCl 2 solution, protected from light for 0.5h; then add 3mL of freshly prepared NaHTe solution, and react at 100°C for 5h to obtain a fluorescence emission wavelength of 580nm, such as fi...

Embodiment 2

[0055] A preparation method of water-soluble Zn-Mg-Te quantum dot / titanium dioxide nanorod composite material, comprising steps as follows:

[0056] S1. Preparation of titanium dioxide nanorods: under an ice-water bath, slowly add 1.8ml of titanium tetrachloride to a beaker containing 19ml of ultrapure water, stir vigorously for 10min to obtain a white suspension, then add 1.8ml of chloroform to the mixture, Stirring was continued for 10 minutes, the mixture was transferred to a polytetrafluoroethylene-lined autoclave, reacted at 180°C for 6 hours, cooled to room temperature, and the white precipitate was collected by centrifugation, washed with deionized water and absolute ethanol to pH 7.0, and then Vacuum dry at 60°C for 12 hours, store at room temperature for later use;

[0057]S2. Amination of titanium dioxide nanorods: place 100 mg of titanium dioxide nanorods prepared in step S1 in a mixture containing 20 ml of ethanol, 1 ml of ammonia solution with a mass concentration...

Embodiment 3

[0065] Such as Figure 8 Shown, a kind of preparation method based on the squamous cell carcinoma photoelectric immunosensor of water-soluble Zn-Mg-Te quantum dot / titanium dioxide nanorod composite material, its steps are as follows:

[0066] (1) bare glassy carbon electrode (diameter 3 mm), polished with 0.3 μm Al2O3 suspension, rinsed with distilled water, and then successively in HNO 3 (formed by mixing equal volumes of 65wt% concentrated nitric acid and water), ethanol (50v / v%), ultrasonically cleaned in twice distilled water, and dried for later use;

[0067] (2) Construction of SCCA photoelectric immunosensor:

[0068] Take 6 μ L of the Zn-Mg-Te / TiO prepared in Example 2 2 The composite material was drip-coated on the surface of the clean glassy carbon electrode obtained in step (1), dried at room temperature, and washed with 0.01 mol / L PBS buffer solution with pH=7.4 to obtain Zn-Mg-Te / TiO 2 / GCE modified electrode;

[0069] Then take 6 μL of 10 μg / mL squamous cell...

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Abstract

The invention discloses a water-soluble Zn-Mg-Te quantum dot and a preparation method of a water-soluble Zn-Mg-Te quantum dot / titanium dioxide nanorod composite material, and belongs to the technicalfields of tumour marker detection, photoelectrochemistry and immune sensing. The water-soluble Zn-Mg-Te quantum dot / titanium dioxide nanorod composite material modifies an L-shaped glassy carbon electrode, the composite material is loaded with a squamous cell carcinoma antibody to form a photoelectric immunosensor, and on the basis of immunological reaction, the specificity of a squamous cell carcinoma antigen is detected by taking white light as a laser light source. The water-soluble Zn-Mg-Te quantum dots, the titanium dioxide nanorods and the white laser source are introduced into the photoelectric squamous cell carcinoma immunization sensing system, and the prepared photoelectric immunosensor has the advantages of being convenient, simple, economic, highly adaptive, simple and rapid inoperation, high in selectivity and sensitivity, and low in detecting limit.

Description

technical field [0001] The present invention also relates to the technical fields of disease marker detection, photoelectrochemistry, and immune sensing, and specifically relates to the preparation and characterization of a novel water-soluble Zn-Mg-Te quantum dot / titanium dioxide nanorod composite material and the relationship between quantum dots and nano-titanium dioxide. Application of Energy Transfer in Squamous Cell Carcinoma Photoimmunosensor. Background technique [0002] According to global cancer statistics, cervical cancer kills about 250,000 women every year, and the vast majority of cervical cancers are of the squamous cell type; squamous cell carcinoma antigen (SCCA) is a TA-4 subtype glycoprotein , has been identified as a tumor marker clinically used to detect cervical cancer. The increase of SCCA content in the body is also related to lung cancer, head and neck cancer, vaginal cancer, liver cancer, etc. The content of SCCA in normal people is extremely low (...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N27/327G01N27/30C01B19/00C01G23/047
Inventor 龙建宇李川洪颖
Owner DONGGUAN UNIV OF TECH
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