Preparation method of compound hydrolyzed protein powder

A technology of compound hydrolysis and protein powder, which is applied in the field of preparation of compound hydrolyzed protein powder, can solve the problems of unsuitable protein digestion function, poor elderly and patients, etc., and achieve the effect of high amino acid utilization rate, rich nutrition, full and efficient utilization

Inactive Publication Date: 2019-05-24
WUHAN POLYTECHNIC UNIVERSITY
View PDF13 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the above-mentioned protein powders currently on the market

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1

[0030] The rice protein and the egg white protein were prepared into a water suspension with a protein concentration of 8% at a mass ratio of 6:4, and the protein was denatured by heat treatment at 100°C for 10 minutes. The homogenization pressure was 30Mpa, and the temperature was 30°C. 3 times, colloid mill micronization treatment 3 times to make the protein particle size below 10μm; adjust the pH of the mixed protein solution to 2-3, temperature 35℃, papain and pepsin in a ratio of 1:1, the amount of enzyme added is that of the substrate protein 2%, stirring speed 30rpm, enzymolysis time 2h, to obtain a protein hydrolysate with a hydrolysis degree of 9%;

[0031] The protein hydrolysate is sterilized by ultra-high temperature at 121℃ for 9 seconds, cooled to 37℃, and the protease hydrolysate is added 10 per kilogram of fermentation broth. 8 The number of viable cfu bacteria, using Bacillus subtilis-yeast-lactic acid bacteria three kinds of joint fermentation, ...

Example Embodiment

[0034] Example 2

[0035] Mix wheat protein, soy protein and duck egg white protein at a mass ratio of 7:2:1 to form a water suspension with a protein concentration of 6%. Heat at 95°C for 15 minutes to denature the protein and make the colloid mill finer. 4 Once again, make the protein particle size to 15μm.

[0036] Adjust the pH of the mixed protein solution to 9.5, the temperature is 45℃, the mass ratio of alkaline protease and trypsin is 1:1, the amount of enzyme added is 3% of the substrate protein, the stirring speed is 50rpm, the enzymolysis time is 4h, and the degree of hydrolysis is 25 % Protein hydrolysate;

[0037] The protein hydrolyzate is sterilized at 95°C for 10 minutes and cooled to 38°C. The protease hydrolyzate is added to 10 per kilogram of fermentation broth. 7 The ratio of the number of cfu viable bacteria is combined fermentation of yeast-lactic acid bacteria, the ratio of yeast-lactic acid bacteria is 1:1, the yeast is grape juice yeast, and the lactic acid ...

Example Embodiment

[0041] Example 3

[0042] Corn gluten, pea protein and oyster protein are mixed evenly according to the mass ratio of 5:4:1, prepared into a water suspension with a protein concentration of 5%, heated at 92°C for 16 minutes to denature the protein, and the high-pressure homogenizer is finely processed. The mass pressure is 40Mpa, the temperature is 50℃, and the number of times is 3 times, so that the size of the protein particles is about 10μm.

[0043] Adjust the pH of the mixed protein solution to pH 7.5, temperature 37°C, neutral protease, papain and ficin in a mass ratio of 2:1:1, the amount of enzyme added is 4% of the substrate protein, stirring speed 20rpm, enzymatic hydrolysis time 3.5h To obtain a protein hydrolysate with a degree of hydrolysis of 20%;

[0044] The protein hydrolysate is sterilized at 123°C for 15 seconds, cooled to 41°C, and the protease hydrolysate is added 5×10 per kilogram of fermentation broth. 6 The ratio of the number of cfu viable bacteria is the co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a preparation method of compound hydrolyzed protein powder. The preparation method specifically includes: subjecting raw material protein to micronization through a high-pressure homogenizer, a colloid mill or the combination of the high-pressure homogenizer and the colloid mill, performing heating treatment to denature the protein, performing single protease or multi-enzymecompound hydrolysis, using bacillus subtilis, yeast and lactic acid bacteria to perform independent or joint fermentation to remove stinking smell, performing ultrafiltration and ion exchange resin desalting, evaluating and supplementing essential amino acid, performing enzymatic polymerization and reconstruction reaction, and drying or mixing after drying to obtain a high-quality protein productwhose essential amino acid proportion is reasonable and amino acid score reaches above 95. The prepared compound hydrolyzed protein powder belongs to high-quality protein, is easy to digest and absorb and suitable for people who needs high-quality protein, elder people with low protein digestion function, people who needs to recover after diseases and people with chronic kidney disease (CKD) andthe like, and has effects of resisting oxidation, lowering cholesterol in blood, strengthening constitution and enhancing immunity.

Description

technical field [0001] The invention relates to the technical field of food raw material processing, in particular to a preparation method of compound hydrolyzed protein powder. Background technique [0002] Protein is an indispensable substance in the human body, the material basis of life, the basic organic matter that constitutes cells, and the main bearer of life activities. Protein is one of the seven essential nutrients for the human body. Protein exists in various parts and cells of the body, and the content in the human body is about 17%-21%. Different proteins have different functions and properties. Protein functions include: forming and repairing human tissues, providing essential amino acids, regulating body fluids and maintaining acid-base balance, synthesizing physiologically active substances, enhancing immunity, and providing energy. There are many sources of protein, including grains, beans, eggs, meat, milk, aquatic products, etc. The Chinese Nutrition ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23J3/00A23J3/34A23L5/20
Inventor 刘良忠陶鑫郑丹妮
Owner WUHAN POLYTECHNIC UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap