A kind of immobilized starter system and its preparation and application

An immobilized fermentation and system technology, applied in the directions of immobilization on or in inorganic carriers, immobilization on/in organic carriers, biochemical equipment and methods, etc., can solve the problems of slow fermentation speed and incomplete degradation.

Active Publication Date: 2021-04-27
SUZHOU BAIYUAN GENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the technical defects of slow enzymolysis and fermentation of straw and incomplete degradation in the prior art, and discloses an immobilized starter system and its preparation and application

Method used

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  • A kind of immobilized starter system and its preparation and application
  • A kind of immobilized starter system and its preparation and application

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specific Embodiment approach

[0029] This embodiment provides a specific implementation of an immobilized fermentation system, which specifically includes the following steps:

[0030] Mix 8g of sodium alginate with 200ml of distilled water, heat and stir to dissolve, and cool to 30°C to make a carrier reagent;

[0031] In a ratio of 400g: 100g: 4000ml glucose, calcium chloride and water were mixed and stirred and dissolved to make a fixation reagent;

[0032] Take the yeast agent and mix it with distilled water to make a live bacteria solution. The concentration of the viable bacteria in the solution is 1.8×10 10 1 / ml, mix 400ml of bacterial liquid with 100ml of carrier reagent, drop the mixed solution in 2L of fixative reagent, soak for 20min, and form sclerotia with a diameter of 2-3mm;

[0033] Take α-amylase, glucoamylase, acid protease, endoglucanase, exoglucanase and β-glucosidase, dissolve them in pure water to make enzyme liquid, and the concentration of each enzyme in the enzyme liquid is 8×10 ...

Embodiment 2

[0035] This embodiment provides a specific implementation of an immobilized fermentation system, which specifically includes the following steps:

[0036] Mix 8g of sodium alginate with 200ml of distilled water, heat and stir to dissolve, cool to 30°C, then add 1.5g of gluconic acid-modified Fe with a diameter of 300-500nm 3 o 4 Stir the magnetic particles evenly to make a carrier reagent;

[0037] In a ratio of 400g: 100g: 4000ml glucose, calcium chloride and water were mixed and stirred and dissolved to make a fixation reagent;

[0038] Take the yeast agent and mix it with distilled water to make a live bacteria solution. The concentration of the viable bacteria in the solution is 1.8×10 10 1 / ml, mix 400ml of bacterial liquid with 100ml of carrier reagent, drop the mixed solution in 2L of fixative reagent, soak for 20min, and form sclerotia with a diameter of 2-3mm;

[0039] Take α-amylase, glucoamylase, acid protease, endoglucanase, exoglucanase and β-glucosidase, dissol...

Embodiment 3

[0041] This embodiment provides a specific implementation of an immobilized fermentation system, which specifically includes the following steps:

[0042]Mix 8g of sodium alginate with 200ml of distilled water, heat and stir to dissolve, cool to 30°C, then add 0.5g of amino acid-modified Fe with a diameter of 500-800nm 3 o 4 Stir the magnetic particles evenly to make a carrier reagent;

[0043] In a ratio of 400g: 100g: 4000ml glucose, calcium chloride and water were mixed and stirred and dissolved to make a fixation reagent;

[0044] Take the yeast agent and mix it with distilled water to make a live bacteria solution. The concentration of the viable bacteria in the solution is 1.8×10 10 1 / ml, mix 400ml of bacterial liquid with 100ml of carrier reagent, drop the mixed solution in 2L of fixative reagent, soak for 20min, and form sclerotia with a diameter of 2-3mm;

[0045] Take α-amylase, glucoamylase, acid protease, endoglucanase, exoglucanase, β-glucosidase, and Aspergill...

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Abstract

The invention relates to the technical field of biochemical industry, in particular to an immobilized fermentation system and its preparation and application; the immobilized fermentation system includes sclerotia and a composite enzyme layer coated on the surface of the sclerotium The enzyme is embedded in a porous carrier, and after immobilization, a functional environment of live bacteria and enzyme is formed, so that the substrate can complete the entire reaction process on the immobilized starter system. The preparation method is simple, and the reagent is green and environmentally friendly. It is used in The straw enzymatic hydrolysis fermentation system can significantly increase the reaction speed and speed, promote the complete reaction, and has good practicability.

Description

technical field [0001] The invention relates to the field of biochemical technology, in particular to an immobilized fermentation system and its preparation and application. Background technique [0002] Straw refers to the remaining stems and leaves of gramineous crops such as rice, wheat, and corn after threshing. China has abundant material resources of crop straw. The traditional incineration method not only wastes a lot of cellulose resources, but also causes serious environmental damage. Pollution. In recent years, in order to rationally utilize cellulose resources, more and more technical means have been applied to the treatment of straw. [0003] The utilization of straw inevitably involves the degradation of straw. The main components of straw include polysaccharide components of the cell wall, such as cellulose, hemicellulose, lignin, pectin, cell wall protein, etc. The final product after hydrolysis is monosaccharide, but the hydrolysis process usually Slow and i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/18C12N11/14C12N11/10C12N11/04C12P19/20C12P19/14C12P19/02C12P39/00C12P7/10C12R1/685
CPCY02E50/10
Inventor 车团结车锐
Owner SUZHOU BAIYUAN GENT CO LTD
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