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Living microbial immobilization microsphere and preparation method and application thereof

A technology of microbial immobilization and microbial activity, applied in the field of environmental pollution microbial treatment, can solve the problems of low binding force between carriers and microorganisms, difficulty in exerting adsorption-accumulation, loss of microbial activity, etc. Highly active effect

Inactive Publication Date: 2019-08-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biochar-immobilized microbial technology that has been reported so far is not effective in the treatment of heavy metal pollution in water, mainly because most of the microorganisms lose their activity during the wastewater treatment process, and it is difficult to play the role of adsorption-accumulation
[0004] Common immobilization methods include adsorption method, embedding method, cross-linking method and covalent binding method. Among them, the adsorption method has the advantages of simple operation, low cost, and little impact on cell activity, but the disadvantage is that the binding force between the carrier and microorganisms is low. , the effect is unstable

Method used

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  • Living microbial immobilization microsphere and preparation method and application thereof
  • Living microbial immobilization microsphere and preparation method and application thereof
  • Living microbial immobilization microsphere and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Immobilized microsphere preparation process and material morphology and properties

[0051] see figure 1 , the preparation steps of the microsphere product are as follows:

[0052] In the first step, after natural air-drying, shredding and grinding, the rice straw was placed in a pyrolysis furnace that was fed with nitrogen gas for 20 minutes in advance, pyrolyzed at a temperature of 700 °C for 2 hours with limited oxygen, cooled to room temperature, ground and sieved to 100 mesh to obtain Microorganism immobilization carrier.

[0053] In the second step, inoculate 1 to 2 loops of Bacillus cereus RC-1 in beef extract peptone body liquid medium, and cultivate and activate at 30°C for 8 to 10 hours at 150 r / min (OD 600 =1.2~1.5), obtain cell suspension; get 5mL cell suspension and mix with 1.5g microbial immobilization carrier, shake culture 2h, make mixed slurry A; Mix slurry A to 100 mL to obtain mixed slurry B.

[0054] In the third step, the mixed slurry...

Embodiment 2

[0059] Example 2 Effect of pH on the adsorption-accumulation of immobilized microspheres

[0060] When the initial Cd 2+ At a concentration of 100 mg / L, for the microbial immobilization carriers (rice straw, chicken manure and sludge) prepared from three different raw materials, immobilized microspheres with a solid-liquid ratio of 0.2 g / L were prepared at different pH (2, 3, 4, 5, 6 and 7) in the beef extract peptone liquid medium (pH is 7.0 ± 0.2), after 24 hours of shaking culture at a shaking speed of 150r / min at room temperature, centrifuge the supernatant (10000rpm, 15min) to measure Cd 2+ concentration.

[0061] from image 3 It can be seen that as the initial pH ranges from 2 to 5, the adsorption-accumulation amounts of the three immobilized microspheres all increase rapidly, then gradually slow down, and finally reach equilibrium. When the initial pH is in the range of 5 to 7, the adsorption-accumulation amount of immobilized microspheres ranges from 91.84 to 124.0...

Embodiment 3

[0063] Example 3 Initial Cd 2+ Effects of concentration on the adsorption-accumulation of immobilized microspheres, the number of microorganisms and the activity of ATPase.

[0064] at different initial Cd 2+ Concentration (5, 10, 20, 50, 80, 100, 110, 120, 150, 180, 200, 210, 220, 250mg / L) in beef extract peptone liquid medium (pH is 7.0 ± 0.2), add solid Liquid ratio is 0.2g / L the living microorganism immobilized microsphere prepared in the embodiment 1, after 24h (150r / min) after constant velocity shaking culture under normal temperature, centrifugation gets supernatant (10000rpm, 15min) and measures Cd 2+ concentration. In addition, the isolated immobilized microspheres were placed in 20mL buffer solution (0.05mol / L Na 2 CO 3 and 0.02mol / L citric acid), shake culture for 1h, after the microspheres are completely dissolved, carry out microbial plate count and ATPase activity determination.

[0065] from Figure 4 a It can be seen that with the initial Cd 2+ With the ...

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Abstract

The invention discloses a living microbial immobilization microsphere and a preparation method and application thereof. The method comprises the steps of microbial immobilization of a carrier, bacterial liquid suspension colonization, microbial active particles, proliferation and reculture and the like. The microsphere is spherical, has pores in the microsphere, has the diameter of 3.0-4.0 mm andthe specific gravity of 1.10-1.20 g / cm<3>, is alkalescent, and has good sedimentation performance and mechanical strength. The microsphere not only can play the chemical adsorption effect of the carrier on cadmium, but also can improve the growth and propagation environment of active microbes, thereby improving the extracellular adsorption and intracellular accumulation effect of active bacteria on cadmium. The maximum adsorption-accumulation amount in the range below initial Cd2 + concentration 250 mg / L can reach 158.77 mg / g. The living microbial immobilization microsphere has the advantagesof adopting cheap and easily available raw materials and being strong in process operability, environmentally friendly, easy to recover remarkable in treatment effect and the like, and has wide application prospect in the biological treatment process of heavy metal polluted water bodies.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of environmental pollution, and relates to a living microorganism immobilized microsphere and its preparation method and application, in particular to a method for immobilizing active bacteria with resistance to heavy metal cadmium, and the use of immobilized bacteria balls in wastewater biological Processing applications. Background technique [0002] Heavy metal cadmium has strong biological toxicity and is not easy to be biodegraded. It can enter human organs through the food chain in the environment, causing teratogenic, carcinogenic, and mutagenic effects, which seriously threaten human health. For water heavy metal cadmium pollution, there are mainly physical chemical methods and biological methods, including chemical precipitation, ion exchange, membrane separation, adsorption separation, plant absorption and microbial adsorption accumulation. At present, the microbial immobili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04C02F3/34C12R1/085C02F101/20
CPCC02F3/34C02F2101/20C12N11/04
Inventor 黄飞李凯颜玉坚王泽煌王衡
Owner SOUTH CHINA AGRI UNIV
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