Vegetable special fertilizer and preparation method and application thereof
A special fertilizer and vegetable technology, applied in the field of special fertilizer for vegetables, can solve the problems of low quality of agricultural products, aggravation of non-point source pollution, soil compaction, etc., and achieve the effect of improving soil structure, increasing yield and quality, and reducing fertilizer application
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Embodiment 1
[0034] The screening of embodiment 1 super efficient nitrogen-fixing bacteria
[0035] 1.1 Preliminary screening of nitrogen-fixing bacteria
[0036] (1) Preparation of sample diluent: Weigh 10 g of the soil sample taken from the soil sample of vegetables grown for many years in Tai'an District, Tai'an City, Shandong Province, and put it into a 250 mL Erlenmeyer flask, add appropriate amount of glass beads and 100 mL of sterile water, and let it soak for 20 minutes , vibrated at 200rpm for 30min in a constant temperature shaker to form a bacterial suspension mother solution, and then diluted with sterile water to obtain 10 -5 、10 -6 、10 -7 dilution of the sample solution.
[0037] (2) Pour plate: Asbestos nitrogen-free solid medium is sterilized, cooled to 60°C, and poured onto the plate.
[0038] (3) Coating plate: 10 -5 、10 -6 、10 -7 Take 100 μL of the diluted sample solution, and use a glass triangular scraper to spread evenly on the medium.
[0039] (4) Cultivation...
Embodiment 2
[0058] The screening of embodiment 2 phosphate solubilizing bacteria
[0059] 1.1 Screening of Phosphorus Solubilizing Bacteria
[0060] The screening method is as follows:
[0061] (1) Preparation of sample dilution: Weigh 10 g of activated sludge sample collected from the wastewater treatment plant of Shandong Huayang Pesticide Chemical Group Co., Ltd. and put it into a 250 mL Erlenmeyer flask, add an appropriate amount of glass beads and 100 mL of sterile water, and let it stand for soaking After 20min, vibrate at 200rpm for 30min in a constant temperature shaker to form a bacterial suspension mother solution, which is then diluted successively with sterile water to obtain 10 -5 、10 -6 、10 -7 dilution of the sample solution.
[0062] (2) Pour plate: After sterilizing the Pikovskaya solid medium, cool to 60°C, pour the plate.
[0063] (3) Coating plate: 10 -5 、10 -6 、10 -7 Take 100 μL of the diluted sample solution, and use a glass triangular scraper to spread evenly o...
Embodiment 3
[0074] Embodiment 3 phosphate solubilizing bacteria (SDTB-0041) is to the degradation of carbonapatite powder
[0075] The seed solution of the phospholytic bacteria (SDTB-0041) obtained by screening was inoculated in the selective culture medium containing carbonapatite powder according to an inoculum size of 5%, and in the selection medium, carbonapatite powder was replaced with carbonapatite powder. Add the medium in the form of powder solution, the content of carbonapatite powder in the carbonapatite powder solution is 35%, and the addition amount of carbonapatite solution in the selection medium is 8‰ (mass ratio); Other components other than phosphorus pesticide solution are 0.1g NaCl, 0.5g MgSO 4 , 0.3g K 2 SO 4 , 0.1 g CaCO 3 , 0.001g FeSO 4 , 1000mL H 2 O, the pH value was adjusted to 7.2, placed in an environment with a temperature of 37° C. and a rotation speed of 200 rpm for 48 hours, and the soluble phosphorus content in the fermentation broth was measured by...
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