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A method for degrading benzopyrene in soil

A benzopyrene and soil technology, applied in the field of combined application of Phomopsis and reeds to degrade benzopyrene in soil, degrading benzopyrene in soil, can solve the problem of incomplete restoration, harsh process requirements, and easy to be affected by the environment and other problems, to achieve the effect of remarkable repair effect, simple process, and environmental friendliness

Active Publication Date: 2021-07-30
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method has high remediation efficiency, but it is prone to secondary pollution, and the remediation is not thorough, requiring secondary treatment; although the extraction method has a high effect, it is not suitable for large-scale soil remediation; currently, the photodegradation method is mainly used in physical degradation , the principle is that visible light can be absorbed by the delocalized large π bond in polycyclic aromatic hydrocarbons to transition to an excited state, and under the action of OH free radicals in water, cracking occurs, but this method is easily affected by the environment during the degradation process. The degradation rate slows down, and the technology of photodegradation method is immature, so it cannot be applied to actual soil remediation on a large scale; although single bioremediation produces few by-products and is environmentally friendly, the repair effect is limited. For example, microbial degradation method has the ability to degrade It has the characteristics of high efficiency, but the disadvantage is that it is single, and it is easily affected by the external environment. At the same time, it has strict requirements on the process. The single phytoremediation mainly uses the characteristics of plant adsorption, which is less affected by the external environment, but the disadvantage is that the efficiency is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] Preparation of bran koji medium: Sieve non-mildew bran to remove fine powder, weigh 8g bran, add it to a 250mL Erlenmeyer flask, add 8mL deionized water, mix well, sterilize at 121°C for 30min, and set aside.

[0024] 2) In the following examples, the Phomopsis sp. used was isolated from the root of reed and identified as Phomopsis sp.

[0025] 3) Determination method of benzopyrene content in soil:

[0026] Take 10g of soil before treatment, remove residues such as sand and stones, dry it naturally in the dark, grind it, sieve it through a fine sieve, and store it at 4°C.

[0027] Put the soil sample into a Soxhlet extractor, heat and reflux with chloroform to extract for 30 minutes, recover the solvent, and use HPLC to measure the content of benzopyrene in it. The measuring method is as follows: C18 chromatographic column, specification 4.6×150mm, 10 μm); The phase is: methanol: water = 90:10, the excitation wavelength of the fluorescent agent detector is 365nm, the ...

Embodiment 1

[0030] A method for degrading benzopyrene in soil, comprising the steps of:

[0031] (1) Select healthy, disease-free reeds, remove the upper and lower parts, segment the reed stalks, and each section has 2-3 sections to obtain reed segments, and then soak the reed segments in 5% rhamnose of benzopyrene In the fat solution, after soaking for 8 days (the germination rate is 85.16%), the reed section is taken out, rinsed with sterile water, and blotted the moisture on the surface with sterile filter paper;

[0032] (2) The reed section is transferred to aseptic water to accelerate germination and cultivate until the buds are 3-5cm long, and set aside;

[0033] (3) Activate Phomopsis, insert it into bran yeast culture medium, culture at 28°C for 5 days, filter, add skim milk powder to the obtained bacteria, the weight ratio of bacteria to skim milk powder is 1:3 , after mixing evenly, freeze-drying, obtains the Phomopsis inoculum (recovering the recovery rate of Phomopsis in the...

Embodiment 2

[0036] In step (1), the concentration of the rhamnolipid solution of benzopyrene is 10%, and the germination rate is 87.45% after soaking for 8 days.

[0037] In step (3), the weight ratio of the thallus to skimmed milk powder is 1:2, and after freeze-drying, the recovery rate of Phomopsis in the Phomopsis inoculum is measured to be 85.33%.

[0038] All the other are identical with embodiment 1.

[0039] The soil benzopyrene degradation rate was measured to be 70.62%.

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PUM

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Abstract

The invention relates to a method for degrading benzopyrene in soil, belonging to the technical field of environmental protection. Select healthy and disease-free reeds, remove the upper and lower parts, segment the reed stems, soak the reed segments in 5‑20% benzopyrene rhamnolipid solution, take out the reed segments after germination, and wash them with sterile water Rinse well and dry the water on the surface; transfer the reed segments into sterile water to accelerate germination until the buds are 3-5cm long; activate Phomopsis, insert it into the bran culture medium, and cultivate it at 28°C for 2-5 days , filter, add skimmed milk powder to the thalline, freeze-dry after mixing evenly, and obtain the Phomopsis inoculum; transplant the reed section in the soil polluted by benzopyrene, and wait until the seedling grows to 10cm long, and the Phomopsis The point mold inoculant is applied to the roots of the reeds and cultivated for 90‑120 days. The method can effectively degrade the benzopyrene in the soil, has a simple process, has a good restoration effect, can realize large-scale restoration of the soil, and is environmentally friendly.

Description

technical field [0001] The invention relates to a method for degrading benzopyrene in soil, in particular to a method for jointly applying Phomopsis and reeds to degrade benzopyrene in soil, and belongs to the technical field of environmental protection. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment and are a class of persistent organic pollutants with strong carcinogenicity and mutagenicity. Human production and life are important causes of polycyclic aromatic hydrocarbons, such as the combustion of fossil fuels, the combustion of organic waste, vehicle exhaust, and petrochemical and coal chemical plant exhaust gases. In agricultural production, straw burning is also an important source of polycyclic aromatic hydrocarbons. [0003] Benzopyrene is a common highly active indirect carcinogen and mutagen in polycyclic aromatic hydrocarbons. It is a toxic substance that has been proven to be carcinogenic, teratogenic, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10
CPCB09C1/10B09C1/105B09C2101/00
Inventor 袁博刘含梦董蒙蒙苏烨阳蒋婉莹
Owner XUZHOU NORMAL UNIVERSITY