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A Strain of Trichosporium and Its Application in Ammonia Nitrogen Degradation in Water

A technology of trichosporon and ammonia nitrogen, which is applied in the field of microorganisms to achieve the effect of low cost, simple equipment and low technical difficulty

Active Publication Date: 2021-04-16
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to published literature, Trichosporon can produce lipase, have the ability to degrade phenol, produce microbial oil and preserve fruit, but there is no report on its use in wastewater treatment to degrade ammonia nitrogen

Method used

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  • A Strain of Trichosporium and Its Application in Ammonia Nitrogen Degradation in Water
  • A Strain of Trichosporium and Its Application in Ammonia Nitrogen Degradation in Water
  • A Strain of Trichosporium and Its Application in Ammonia Nitrogen Degradation in Water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028]Isolation and Identification of Trichosporon Monteense W02:

[0029]Water samples were collected from the US Iowa State University Food Plastics, and water samples were turbid in the oxygen enrichment culture of 30 ° C in a glucose (YPD) liquid medium containing 50 μg / ml ampicillin. After proper dilution Painting the flat plate, according to the form of the colony, a typical yeast single colony, by mirror-check, the ammonia nitrogen degradation ability is measured, and the strain monochrome falls, and inoculates the constant temperature of 30 ° C in YPD liquid medium. 48h. The total DNA of strain W02 is a template, and the ITS sequence of the strain is amplified under the guided PCR of primer ITS1 (TCCGTGTGAACCTGGG) and ITS4 (TcctcgctTttgatgc), and the PCR reaction system is 50 μl, MIX25 μL, ITS1 1 μL, ITS41 μL, DDH2O2 1 μL, 2 μl of the template, a PCR reaction condition 95 ° C 30s, 50 ° C 30s, 72 ° C 60s, and the number of cycles was 31 times. After the end of the reaction, 1%...

Embodiment 2

[0032]Liquid fermentation culture of the Trichosporon Montevideense W02:

[0033]The W02 strain was seeded in a 2% inoculation amount (V / V) in YPD liquid medium (protein 20g, yeast extract 10g, glucose 20g, water 1000 mL, sterilization of 20 min, 500 ml of triangular bottle). 150 rpm temperature culture at 30 ° C, sampling once every 2h, 100 times dilution with sterile water, and detected a blank control at an unfiltered medium, detecting the absorbed value at the wavelength of OD600NM, with the culture time as the horizontal coordinate, the OD value is vertical The coordinates are mapped to the growth curve of yeast. The strain grows rapidly in YPD medium, and the culture is about 12 h and the maximum biomass (image 3 ).

Embodiment 3

[0035]Effects of different carbon sources on ammonia nitrogen degradation:

[0036]The spore yeast W02 was cultured in a 2% inoculation amount (V / V) to 50 ml of YPD liquid medium at 30 ° C for 24 h, centrifugal removal medium for 50 ml of sterile water resuscitation, and the number of live bacteria after resuspend is 5 100 million / ml.

[0037]The 1.0 ml of hemacrobacterial fluid is drawn to 100 ml of 45 mg / L ammonia nitrogen-sterilized artificial sewage (0.5 g of hydrogen phosphate, 0.5 g of magnesium sulfate, potassium chloride 0.01g, iron sulfate 0.01 g, water 1000ml, Add Ammonium chloride to final concentration 45 mg / L), add end concentration 2 g / l glucose, sucrose, ethanol, sodium acetate, sodium citrate as carbon sources, and no carbon source as a control group, 3 repetitions, 25 ° C The ammonia nitrogen concentration was measured after 150 rpm culture for 24 h. It can be seen from Table 1, glucose and sucrose are good carbon sources for ammonia nitrogen degradation of stra...

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Abstract

The invention discloses a strain of Trichosporium yeast and its application in the degradation of ammonia nitrogen in water bodies. The applicant screened a strain of Trichosporium yeast from an algal sewage reactor, and found through experimental testing that the Trichosporium yeast has good ammonia nitrogen degradation ability , the strain has been sent to the China Center for Type Culture Collection, preservation number: CCTCC NO: M2018909. Ammonia nitrogen degradation experiments in aquaculture wastewater and domestic sewage show that the strain can effectively and rapidly degrade the concentration of ammonia nitrogen in water, and has broad application prospects and potential for popularization and application.

Description

Technical field[0001]The invention belongs to the microbial field, and specifically, a strain yeast strain and its application in the degradation of water ammonia nitrogen.Background technique[0002]The rapid growth of the population and the contaminants brought by the economic development of the index will inevitably enter the rivers and lakes, causing water pollution and eutrophication. Water pollution has seriously restrictive economic development and threatens the safety of the public.[0003]Ammonia nitrogen is the most wide source of inorganic contaminants in wastewater. In recent decades, due to the rapid development of industrial and agriculture, residents' sewage, urban garbage, agricultural ammonitritious fertilizer, industrial wastewater discharge, so that the ammonia nitrogen in many natural water in my country has exceeded the scope of the natural environment. Excessive ammonia nitrogen has a nutritional phenomenon of various natural water bodies, including the main lake r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C02F3/34C02F101/16C02F101/38C02F103/20C12R1/645
CPCC02F3/347C02F2101/16C02F2101/163C02F2101/166C02F2101/38C02F2103/20C12N1/16C12N1/145C12R2001/645
Inventor 赵述淼温志友崔雨欣梁运祥葛向阳彭楠梅余霞胡远亮
Owner HUAZHONG AGRI UNIV
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