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Drug carrier system based on fluorescence resonance energy transfer of carbon dots and hollow manganese dioxide

A technology of fluorescence resonance energy and manganese dioxide, which is applied in drug combinations, antineoplastic drugs, pharmaceutical formulations, etc., can solve problems that are difficult to remove, affect life activities, and affect the effect of treatment, so as to improve drug utilization and reduce Antioxidant, reducing toxic and side effects

Active Publication Date: 2020-01-03
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many other drug carriers, which will continue to accumulate in the body after producing corresponding effects, and are difficult to be cleared by the human body, affecting normal life activities and the effect of follow-up treatment

Method used

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  • Drug carrier system based on fluorescence resonance energy transfer of carbon dots and hollow manganese dioxide
  • Drug carrier system based on fluorescence resonance energy transfer of carbon dots and hollow manganese dioxide
  • Drug carrier system based on fluorescence resonance energy transfer of carbon dots and hollow manganese dioxide

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preparation example Construction

[0042] (2). Preparation of manganese dioxide with core and template:

[0043] Measure 50-80mL of deionized water, 50-80mL of ethanol and 1-3mL of ammonia water, mix well, add 500-1000mg of cetyltrimethylammonium bromide (CTAB) into it, ultrasonically disperse, and at the same time Weigh 100-400 mg of solid nano-silicon spheres obtained in step (1), ultrasonically disperse them into 30-60 mL of deionized water, add the aqueous solution of solid nano-silicon spheres to the above mixed solution, stir at room temperature for 30 minutes, and then add 100- 400mg potassium permanganate (KMnO 4 ), continue stirring at room temperature for 5-12 hours, then centrifuge (10000rpm / min×10min) to precipitate, wash with deionized water and ethanol repeatedly three times, and vacuum-dry for later use.

[0044] (3). Remove the solid nano-silicon balls:

[0045] Redisperse 300-500 mg of the product in step (2) into 20-80 mL of deionized water, add 500-1500 mg of sodium carbonate (Na 2 CO 3 )...

Embodiment 1

[0054] A drug carrier system based on fluorescence resonance energy transfer of carbon dots and hollow manganese dioxide, the specific steps are as follows:

[0055] Step 1. Preparation of blue light carbon dot CDs

[0056] Weigh 1.0507g of citric acid (CA) and ultrasonically disperse it into 10mL of deionized water, add 335 microliters of ethylenediamine to the above solution with a pipette gun, then transfer the solution to a 20mL polytetrafluoroethylene liner, Put it into a 20mL polytetrafluoroethylene autoclave, react at 200°C for 5h, wait for it to cool naturally, use a dialysis bag with a molecular weight of 1000 for two days, and then vacuum dry the product at 60°C for 12h after rotary evaporation to obtain Blue light carbon quantum dots.

[0057] Depend on Figure 4 It can be seen that the optimal emission spectrum of the blue-light carbon quantum dots provided in this embodiment is 400-450 nm.

[0058] Step 2, preparation of hollow manganese dioxide (H-MnO 2 )

...

Embodiment 2

[0084] A drug carrier system based on fluorescence resonance energy transfer of carbon dots and hollow manganese dioxide, the specific steps are as follows:

[0085] Step 1, branched polyethyleneimine (PEI) coated hollow manganese dioxide (H-MnO2):

[0086] First prepare 300mL0.01mol / L Tris HCl buffer solution and adjust its pH to 7.4; then weigh 2000mgPEI and dissolve it in 50mL Tris HCl buffer solution, and after ultrasonic dispersion, obtain PEI solution for use; weigh 200mgH-MnO 2 Ultrasonic disperse into 300mL Tris HCl buffer solution, add 100mgDOX in a dark environment, and stir at room temperature for 24h. After the reaction was completed, the stirring was accelerated, and the PEI solution was slowly added dropwise to the H-MnO 2 In the solution, after all the PEI solution is added dropwise, adjust the pH of the system to 7.4, stir rapidly at room temperature for 4 hours, then centrifuge (10000rpm / min×10min) to precipitate, wash repeatedly with deionized water and etha...

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Abstract

The invention discloses a drug carrier system based on the fluorescence resonance energy transfer of carbon dots and hollow manganese dioxide. The biodegradable hollow manganese dioxide is used as a carrier to load a model drug of doxorubicin, then branched polymine coats the hollow manganese dioxide to block the pores of the hollow manganese dioxide, and then the carbon dots are modified on the surface of the hollow manganese dioxide. In the drug carrier system, H-MnO2 and the carbon dots undergo fluorescence resonance energy transfer, so that the fluorescence of the carbon dots is quenched;when the drug carrier system reaches tumor cells under the EPR effect, in the tumor microenvironment, the H-MnO2 is degraded by the high concentration of glutathione in the tumor cells, thus the fluorescence resonance energy transfer between the carbon dots and the H-MnO2 is block, and thus fluorescence recovery of the carbon dots is realized.

Description

technical field [0001] The invention specifically relates to a drug carrier system based on fluorescence resonance energy transfer of carbon dots and hollow manganese dioxide, and belongs to the field of drug carriers. Background technique [0002] Cancer is one of the most destructive diseases in the world, and chemotherapy is one of the most widely used treatment methods at this stage. However, in conventional cancer chemotherapy, while highly toxic drugs act on the whole body and cause strong toxic and side effects, the efficacy of tumor drugs is greatly reduced. [0003] In recent years, the research progress of ROS-based nanotherapy technology, through nanomaterials to generate or scavenge reactive oxygen species to improve the therapeutic effect. ROS, including superoxide anion, hydrogen peroxide (H 2 o 2 ), singlet oxygen and hydroxyl radicals (·OH), have the ability to kill cancer cells and destroy biomolecules such as lipids, proteins and DNA. The development of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/02A61K47/04A61K47/34A61K31/704A61K41/00A61P35/00A61K49/00
CPCA61K47/02A61K47/34A61K31/704A61K41/0057A61P35/00A61K49/0019A61K2300/00
Inventor 许子强何航张书媛李草陈学琴江兵兵
Owner HUBEI UNIV
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