Deferoxamine-loaded artificial periosteal and preparation method thereof

An artificial bone and electrospinning technology, applied in the field of biomedical materials, can solve the problems of limited release of DFO, difficulty in ensuring the demand of bone tissue, etc., to prevent infection and heterotopic ossification, prolong drug action time, and improve The effect of biocompatibility

Active Publication Date: 2020-01-10
北京市创伤骨科研究所
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Problems solved by technology

However, the materials loaded with DFO in the current research are only limited to the adsorption of DFO molecules or the use of chemical bonds to connect to the surface of the scaffold material, the release of DFO is limited, and there is a problem of burst release, which is difficult to meet the needs of bone tissue repair in the later stage (Deng Lianfu, Chen et al. Hao, Qi Jin, Cui Wenguo. An external dressing and its preparation method and application. 2016-10-12)
[0004] At present, there is no "artificial periosteum" product used clinically. The artificial periosteum in the existing research includes: ① mineralized collagen artificial periosteum prepared by using nano-calcium phosphate and collagen fibers through processes such as pressing and freeze-drying (Tao Chunsheng, Qiu Zhiye, Song Tianxi, Zhang Ziqiang, Cui Fuzhai. A mineralized collagen artificial periosteum and its preparation method. 2014-10-15.); ②Degradable polymer porous membrane loaded with BMP, VEGF and other bioactive factors prepared by electrospray technology (Wang Shenguo, Wang Honghe, Liu Hao, Ren Hongwen. Bioabsorbable artificial periosteum and its preparation method. 2008-02-27.); ③ Electrospun membrane loaded with perfluorocarbon polymer particles and capable of increasing oxygen (He Dannong , Wang Ping, Zhu Jun, Wang Jie, Zhu Hai'e, Jin Caihong. Periosteum material with tissue oxygenation function and its preparation method and application. 2018-03-20.), etc., there is no artificial periosteum with slow-release DFO function Research and Related Products

Method used

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  • Deferoxamine-loaded artificial periosteal and preparation method thereof
  • Deferoxamine-loaded artificial periosteal and preparation method thereof
  • Deferoxamine-loaded artificial periosteal and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Example 1 Preparation of DFO-collagen blended artificial periosteum

[0049] Step 1. Dissolve 1.6g of medical grade type I collagen powder in 20mL of hexafluoroisopropanol, and stir magnetically at 37°C for 48 hours to fully dissolve to obtain a uniform collagen solution;

[0050] Step 2, adding DFO to the collagen solution obtained in Step 1, the amount added is 1% of the total mass of the solution, stirring evenly with a magnetic force at 37°C to obtain an electrospinning solution;

[0051] Step 3, electrospinning the solution obtained in step 2, using the stainless steel drum as the receiving device, the drum rotation rate is 600rpm, the spinning solution flow rate is 1.0mL / h, the voltage is 22kV, the receiving distance is 150mm, and the spinning time is 20h. An electrospun fiber membrane with a thickness of about 450 μm was obtained;

[0052] Step 4, placing the DFO-collagen blended fiber membrane obtained in step 3 at room temperature in a fume hood for 2 days to ...

Embodiment 2

[0057] Step 1: Dissolve 1.6g of gelatin powder in 20mL of acetic acid, stir magnetically at 37°C for 48 hours to fully dissolve to obtain a uniform gelatin solution;

[0058] Step 2, adding DFO to the gelatin solution obtained in step 1, the amount added is 0.6% of the total mass of the solution, stirring evenly with a magnetic force at 37°C to obtain an electrospinning solution;

[0059] Step 3, electrospinning the solution obtained in step 2, using the stainless steel drum as the receiving device, the drum rotation rate is 500rpm, the spinning solution flow rate is 3.0mL / h, the voltage is 15kV, the receiving distance is 200mm, and the spinning time is 8h. An electrospun fiber membrane with a thickness of about 200 μm is obtained;

[0060] Step 4, the DFO-gelatin blended electrospun fiber membrane obtained in step 3 is placed in a fume hood at room temperature for 3 days, and DFO is bonded to its surface with carbonyldiimidazole;

[0061] In step 5, the fibrous membrane obta...

Embodiment 3

[0063] Step 1, dissolving 1.6g of chitosan powder in 20mL of trifluoroethanol, stirring magnetically at 37°C for 48 hours to fully dissolve it to obtain a uniform chitosan solution;

[0064] Step 2, adding DFO to the chitosan solution obtained in step 1, the amount added is 0.8% of the total mass of the solution, and magnetically stirred at 37°C to obtain an electrospinning solution;

[0065] Step 3, electrospinning the solution obtained in step 2, using the stainless steel drum as the receiving device, the drum rotation rate is 300rpm, the spinning solution flow rate is 10mL / h, the voltage is 7kV, the receiving distance is 80mm, and the spinning time is 6h, and the obtained An electrospun fibrous membrane with a thickness of about 300 μm;

[0066] Step 4, place the DFO-chitosan blended electrospun fiber membrane obtained in step 3 at room temperature in a fume hood for 4 days, and use carbonyldiimidazole to bond DFO to its surface;

[0067] In step 5, the fiber membrane obta...

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Abstract

The invention discloses a DFO-loaded artificial periosteal and a preparation method thereof, and belongs to the field of orthopedic implant materials. According to the DFO-loaded artificial periosteal, degradable polymer is used as a main raw material, DFO molecules with an osteogenic function are added, the DFO-high-polymer-material blending artificial periosteal is prepared by an electrostatic spinning method, through fiber blending, surface adsorption and / or loading, a polymeric microsphere is added, the mixture is mixed with a degradable high polymer material solution, and then DFO with amino is bonded to the surface of the DFO-high-polymer-material blending artificial periosteal by using chemical bonds in a selective use mode to achieve the effect of slow and long-lasting release. TheDFO-loaded artificial periosteal has good osteogenic activity, forms a barrier locally in the defect and can effectively prevent the invasion of pathogenic organisms and the occurrence of ectopic ossification, and the artificial periosteal has good biocompatibility, mechanical strength and degradability and can be used as a graft repair material for bone tissue defects.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, in particular to an artificial periosteum loaded with deferoxamine (DFO) and a preparation method thereof. Background technique [0002] Bone defect is a common clinical disease in orthopedics, which can be caused by various factors such as trauma, infection and tumor, and is one of the difficult problems in orthopedics treatment. At present, the commonly used clinical treatment method is bone grafting, which uses bone scaffolds to fill bone defects and induce bone regeneration. However, after the bone scaffold is implanted, its repair process is basically a passive connection and filling process, lacking active perception of the defect environment. Although some stents can promote angiogenesis and bone regeneration with the help of exogenous cytokines, it is still difficult to simulate the bone remodeling process in vivo, so no satisfactory therapeutic effect has been obtained (T.G...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/54A61L27/58D01D5/00
CPCA61L27/44A61L27/54A61L27/58A61L2300/204A61L2300/412A61L2300/604A61L2430/02D01D5/0038D01D5/0076
Inventor 石锐李伟阳田伟
Owner 北京市创伤骨科研究所
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