Preparation method of solidified fermented milk rich in bifidobacterium animalis and fermented milk

Abstract

The invention provides a preparation method of solidified fermented milk rich in bifidobacterium animalis, which comprises the following steps: (1) inoculating zymophyte into raw milk, and carrying out full stirring and uniform mixing; (2) inoculating bifidobacterium animalis within 0.2-0.5h, and carrying out full stirring and uniform mixing; and (3) after sterile filling, carrying out standing and fermenting to obtain fermented milk, and refrigerating and curing the fermented milk to obtain the solidified fermented milk rich in bifidobacterium animalis. According to the preparation method ofsolidified fermented milk rich in bifidobacterium animalis provided by the invention, the effect of changing the inoculation time to promote the growth of bifidobacterium animalis is found through a step-by-step fermentation experiment. Because bifidobacteria are added to the milk medium system within 0.2-0.5h, small molecular peptides or other factors generated by metabolism of zymophyte can be utilized by the bifidobacteria, and rapid proliferation of the bifidobacteria is realized. Normal milk coagulation time and milk coagulation state are guaranteed, and the viable count of the bifidobacteria animalis can reach 108CFU / mL or above after fermentation.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for preparing solidified fermented milk rich in animal bifidobacteria and the fermented milk. Background technique [0002] Gastrointestinal diseases are common and frequently-occurring diseases, and the total incidence accounts for about 20% of the population. The older you are, the higher the incidence rate, especially in middle-aged and elderly people over 50 years old. It has been confirmed that Helicobacter pylori is closely related to the four diseases of upper gastrointestinal tract chronic gastritis, peptic ulcer, gastric cancer, and lymphoma, and about 67-80% of gastric ulcers and 95% of twelve ulcers are caused by it , spreads widely and very quickly, and its infection rate even surpasses that of hepatitis B virus. Effectively inhibiting its harmful effects on the pylorus of the human stomach is crucial to human gastrointestinal heal...

AI Technical Summary

Problems solved by technology

[0005] However, the large-scale application of bifidobacteria in the dairy field still faces a major technical problem. It is difficult for the discovered bifidobacteria strains to grow or maintain effective bacterial activity in the milk medium. Bacteria, the special metabolites of certain fermenting bacteria can be absorbed and utilized by Bifidobacteria, and at the same time, the rate of fermentation acid production is appropriate, and the degree of post-acidification is weak, so as to provide a suitable environment for the growth and preservation of Bifidobacteria, but the excess of fermenting bacteria Adding will also form a competitive relationship with the proliferation of bifidobacteria, which is not conducive to the proliferation of bifidobacteria

Therefore, it is necessary to try to construct a new type of fermented milk system that is conducive to the proliferation of functional probiotics, such as adding other lactic acid bacteria with coordinating effects, growth factors, and growth-promoting prebiotic factors to the fermentation system. Although the above research has some technological breakthroughs, However, since the flavor of fermented milk cannot be effectively guaranteed, it has great limitations in actual production and application.

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