Isoptericola salitolerans and application of isoptericola salitolerans in production of seaweed liquid fertilizer

A technology of seaweed liquid and termites, applied in the field of functional fermentation strain screening, can solve the problems of inability to release active ingredients, poor salt tolerance, unsatisfactory degradation effect of macromolecular polysaccharides, etc., and achieve the effect of promoting plant growth

Active Publication Date: 2020-06-09
荣成市泓派海洋生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, there are two problems with the strains used to produce seaweed fertilizer from kelp: (1) In terms of salt tolerance, some strains need to add sodium chloride or seawater to grow; while some strains have poor salt tolerance, Dried kelp cannot be fermented directly
(2) Seaweed polysaccharide is a natural soil conditioner. The strains currently used are mainly spores, lactic acid bacteria, and yeasts. The degradation effect of macromolecular polysaccharides in kelp is not ideal, and the active ingredients in kelp cannot be released as much as possible. Come out, the content of seaweed polysaccharide in kelp fermentation broth is not high

Method used

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  • Isoptericola salitolerans and application of isoptericola salitolerans in production of seaweed liquid fertilizer
  • Isoptericola salitolerans and application of isoptericola salitolerans in production of seaweed liquid fertilizer
  • Isoptericola salitolerans and application of isoptericola salitolerans in production of seaweed liquid fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Screening and Identification of Salt-tolerant Termites ISO-49

[0042] Step 1: Collect Samples

[0043]Seawater, sediment and kelp samples were collected from 9 different kelp cultivation areas in Rongcheng. The seawater samples were directly separated by the dilution coating method. Sea mud and kelp samples need to be diluted and shaken before being separated by dilution coating method.

[0044] The specific operation is as follows:

[0045] Take 10 grams of sea mud and 10 grams of kelp samples (cut into small pieces with sterile scissors in advance), add them to 90 mL of sterile normal saline, shake at room temperature for 2 hours at 150 r / min to make a sample solution.

[0046] Take 0.1mL of the sample solution and spread it directly on the 2216E medium (Difco, product number: 212185), place it upside down at 30°C and culture it for 5 days, pick the single colonies grown on the plate, and perform streak purification on the 2216E medium plate . A total...

Embodiment 2

[0064] Example 2: Ability to produce alginate lyase from the salt-tolerant termite fungus ISO-49

[0065] The method for measuring the activity of alginate lyase is as follows:

[0066] The activity of alginate lyase was detected by 3,5-dinitrosalicylic acid (DNS) colorimetric method. The increasing amount of reducing sugar in the reaction solution is used as the detection index of enzyme activity, and the increasing amount of reducing sugar is determined by DNS reagent. Glucose was used as a standard to make a standard curve, and the amount of reducing sugar generated was calculated according to the difference in absorbance between the reaction group and the control group. One unit of enzyme activity is defined as the amount of enzyme required for the reaction solution to produce 1 μg of reducing sugar per minute under the above conditions.

[0067] Take 0.1 mL of centrifuged fermentation supernatant (crude enzyme liquid), mix with 0.9 mL of 0.3% sodium alginate solution (d...

Embodiment 3

[0076] Example 3: Detection of the ability of fucoidan in fermented kelp by the salt-tolerant termite fungus ISO-49

[0077] Step 1: Crush the dried kelp to a 60-mesh sieve and store at 4°C.

[0078] Step 2: inoculate the strain ISO-49 in the re-screened liquid seed medium, culture at a temperature of 30° C., place on a shaker and culture at a speed of 150 r / min for 24 hours, and prepare the fermentation broth of the strain ISO-49.

[0079] Step 3: Weigh 100g of kelp powder, 10g of sodium alginate, 5g of potassium dihydrogen phosphate, and 5g of magnesium sulfate, then add 800mL of water, stir evenly, adjust the pH to 6.0, and sterilize at 121°C for 20 minutes.

[0080] Step 4: Add 100mL of ISO-49 fermentation broth, and ferment at 30°C for 6 days to make kelp liquid fertilizer.

[0081] In the control group, 100 ml of sterile liquid seed medium was used instead of adding bacterial solution.

[0082] Step 5: Determining the content of fucoidan in the kelp solution after ferm...

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Abstract

The invention provides a strain capable of degrading kelp. The strain is an isoptericola salitolerans ISO-49 strain, and the preservation number is CGMCC No.18975. The isoptericola salitolerans ISO-49provided by the invention can be used for fermenting the kelp to produce a seaweed liquid fertilizer. The strain ISO-49 used in the invention has very strong ability to produce alginate lyase, and can quickly destroy the structure of kelp cell walls to release nutrient substances of the kelp; the produced alginate lyase can decompose alginate polysaccharides in the kelp and make the alginate polysaccharides degraded into small-molecule substances that can be easily absorbed and utilized; and the yield of seaweed polysaccharides after fermentation is much higher than a yield of seaweed polysaccharides extracted by a general enzymatic method.

Description

technical field [0001] The invention belongs to the technical field of functional fermentation strain screening, and in particular relates to a salt-tolerant termite fungus and its application in the production of seaweed liquid fertilizer. Background technique [0002] Seaweed fertilizer is mainly a bio-fertilizer made by scientifically processing large economical seaweeds (macroalgae, kelp, sargasso, kelp and Ascophyllum nodosum, etc.) as the main raw materials. As a pure natural seaweed extract, seaweed fertilizer retains to a great extent the bioactive substances that are beneficial to plant growth and mineral nutrients enriched in seaweed, including seaweed polysaccharides, phenolic substances, mannitol, beet Alkali, plant growth-promoting substances (auuxin, cytokinin, gibberellin and abscisic acid, etc.), and trace elements such as nitrogen, phosphorus, potassium, iron and iodine. Among them, seaweed polysaccharide is the main component of kelp algae, which has a var...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05G3/00C05G3/40C05G3/80C05F17/20C05F17/50C05G5/20C12R1/645
CPCC12N1/14C05B7/00C05G3/00C12R2001/645C12N1/145Y02W30/40
Inventor 张德超许静媛张德进宋永科王灵华许福土
Owner 荣成市泓派海洋生物科技有限公司
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