microbacterium and its application

A microbacillus and microbial agent technology, applied in the field of environmental microorganisms, can solve the problems of secondary pollution of the environment by chemical methods, inadequate sewage treatment facilities, insufficient supervision and control, etc., and achieve excellent sewage treatment effects, outstanding comprehensive performance, The effect of a long growth cycle

Active Publication Date: 2022-05-03
JIANGSU ZHAOJUN NEW MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, my country's sewage treatment facilities are not perfect, and the supervision and control are not effective enough, which makes the main discharge sites of domestic sewage and industrial wastewater - urban rivers, the load of organic carbon pollutants, organic nitrogen pollutants, and phosphorus-containing compounds continues to increase. , the water body is black and smelly, blue-green algae and other aquatic plants frequently break out, and the situation is grim. In order to protect the health of urban residents and the sustainable development of social economy, water resource protection and management has become an urgent need for economic development and ecological environment.
[0003] At present, the protection and management of water resources are mainly physical and chemical means. Although the method is effective, it needs a lot of manpower and financial resources, and the chemical method will also cause secondary pollution to the environment to varying degrees.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation and screening of Exiguobacterium sp. ZJ-S001-12

[0031] Collect 7 parts of activated sludge and water samples from the breeding pond, weigh 5g samples respectively (5mL for water samples) and add them to a 150mL Erlenmeyer flask containing LB liquid medium, shake in a shaker at 30°C and 220r / min Cultivate overnight, and dilute the sample solution by a certain number of times using the ten-fold dilution method, and set aside. Take 0.1 mL of diluted sample solution and spread it on solid LB medium, incubate at 30°C for 48 hours, select the colonies with good growth, similar colony shape and the largest number, separate and purify until the single bacteria with the same colony shape. A strain ZJ-S001-12 was screened out.

Embodiment 2

[0032] Example 2 Identification of Exiguobacterium sp. ZJ-S001-12

[0033] (1) Morphological characteristics

[0034] After the strain ZJ-S001-12 was cultured on the solid LB growth medium for 24 hours, the colony was orange-yellow, with a smooth surface, moist and shiny, and the cells of the strain were observed under the microscope to be Brevibacteria, such as figure 1 shown.

[0035] (2) Physiological and biochemical identification

[0036] The physiological and biochemical characteristics of strain ZJ-S001-12 were identified, and the results are shown in Table 1.

[0037] Table 1

[0038] Biochemical Indicators result Biochemical Indicators result glucose fermentation + lactose fermentation - Mannitol Fermentation - maltose fermentation + sucrose - indole - MR - VP - gelatin liquefaction + starch hydrolysis + hydrogen sulfide - Urease -

[0039] Instructions: "+": positive response; "-" negative ...

Embodiment 3

[0058] Example 3 Optimization of Growth Conditions for Exiguobacterium sp. ZJ-S001-12

[0059] Culture medium (solvent is water): peptone 10g / L, yeast powder 5g / L, NaCl 5g / L, pH 7.2-7.6.

[0060] (1) Optimum growth pH of the inventive bacterial strain

[0061] Use 1.0mol / L of HCl or 3.0mol / L of NaOH to adjust the initial pH of the culture solution to 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, 11, according to 1% (v / v ) inoculum amount into strain ZJ-S001-12, cultivated at 35°C and 220r / min, and measured its absorbance at 600nm with a UV-visible spectrophotometer after 24 hours, and the culture solution without inoculation was used as a blank control.

[0062] Depend on image 3 It is known that the growth rate of the strain increases gradually when the pH increases from 6.5 to 8.5. After continuing to increase the pH, the growth rate gradually decreased. Using Sigmaplot software to perform nonlinear fitting on the above figure, the optimum growth pH of the strain can be obtain...

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Abstract

The invention discloses an Exiguobacterium sp. and its application. The strain is named Exiguobacterium sp. ZJ-S001-12, which has been preserved in the Chinese Typical Culture Collection located at Wuhan University in Wuhan, China on September 25, 2018. Center (CCTCC for short), the deposit number is CCTCC NO: M 2018654. The present invention also provides the application of the microbacterium CCTCC NO: M 2018654 in wastewater treatment. The test results show that the microbacterium ZJ‑S001‑12 is resistant to high temperature, alkali and high salt, has a long growth cycle, and can effectively improve the Water quality, reduce COD, ammonia nitrogen, total phosphorus in water, efficient and pollution-free.

Description

technical field [0001] The invention relates to environmental microorganisms, in particular to a microbacterium and its application. Background technique [0002] With the progress of the times and the development of society, the living standards of our people continue to improve, the demand for water resources is increasing, and the discharge of corresponding residential sewage and industrial wastewater is also increasing. However, my country's sewage treatment facilities are not perfect, and the supervision and control are not effective enough, which makes the main discharge sites of domestic sewage and industrial wastewater - urban rivers, the load of organic carbon pollutants, organic nitrogen pollutants, and phosphorus-containing compounds continues to increase. , the water body is black and smelly, and aquatic plants such as blue-green algae frequently break out. The situation is grim. In order to protect the health of urban residents and the sustainable development of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC12N1/20C02F3/34C12R2001/01C12N1/205
Inventor 鲁平才谢柳
Owner JIANGSU ZHAOJUN NEW MATERIAL CO LTD
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