Method for increasing yield of outer membrane vesicles (OMVs) of bacteria and application of OMVs of bacteria

A technology of outer membrane vesicles and bacteria, applied in the biological field, can solve the problems of small secretion, high cost, limited use and promotion, etc., and achieve the effect of enhancing immune effect, reducing production cost, and good immune enhancing function
CN111454979AActive Publication Date: 2020-07-28JIANGSU ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU ACAD OF AGRI SCI
Publication Date
2020-07-28

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Abstract

The invention provides a method for increasing the yield of outer membrane vesicles (OMVs) of bacteria and application of the OMVs of bacteria, and belongs to the technical field of biology. The method comprises the step of culturing a gram-negative bacteria mutant strain with an yjeE gene knocked out. The invention further provides application of the OMVs, prepared by the method, of the bacteriain preparation of a vaccine and an immunoenhancement. By use of the method, the yield of the OMVs of Escherichia coli reaches 701 mg / L, is increased by about 100 times than the culture yield of an original strain in an LB culture medium, the method is application to mass production of the OMVs in the pharmaceutical industry, and the production cost is significantly reduced. The OMVs, prepared by the method, of the bacteria have better immunoenhancement functions, can be added to common vaccines and can significantly enhance the immune effects.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the yield of bacterial outer membrane vesicles and an application thereof. Background technique

[0002] Outer membrane vesicles (Outer membrane vesicles, OMVs) are the products secreted by budding on the surface of Gram-negative bacteria. -300nm range. This structure includes lipopolysaccharide (LPS), outer membrane proteins, phospholipids, bacterial toxins, adhesins, DNA, and periplasmic components that are enveloped by the outer membrane during formation. Because outer membrane vesicles cannot replicate and contain a large number of bacterial antigens, they have good immunogenicity and immune enhancement effects, and can activate the host cell immune system. Therefore, outer membrane vesicles can be used as a new type of bacterial vaccine or immune enhancer with great potential.

[0003] However, outer membrane vesicles are carriers for bacte...

Claims

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