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Construction and application of aptamer sensor taking nanogold/zinc oxide-graphene composite material as photoelectric sensitive element

An aptamer sensor and composite material technology, which is applied in the direction of material electrochemical variables, material analysis, and material analysis through electromagnetic means, can solve the problems of complex sample pretreatment process, difficult to achieve rapid detection, and high detection cost, and achieve Good stability and sensitivity, convenient operation and simple preparation process

Pending Publication Date: 2020-08-21
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are many detection methods for Cd(II), including atomic absorption spectrometry, ultraviolet spectrophotometry, inductively coupled plasma mass spectrometry and inductively coupled plasma atomic emission spectrometry, etc. These methods have high sensitivity and accuracy. Reliability, but the equipment is expensive, the detection cost is high, the sample pretreatment process is complicated, and it is difficult to achieve the purpose of rapid detection

Method used

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  • Construction and application of aptamer sensor taking nanogold/zinc oxide-graphene composite material as photoelectric sensitive element
  • Construction and application of aptamer sensor taking nanogold/zinc oxide-graphene composite material as photoelectric sensitive element
  • Construction and application of aptamer sensor taking nanogold/zinc oxide-graphene composite material as photoelectric sensitive element

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for constructing a photoelectrochemical aptasensor using nano-gold / zinc oxide-reduced graphene oxide ternary composite material as a photosensitive element, comprising the steps of:

[0032] (1) Preparation of photoelectrodes:

[0033] (1.1) Preparation of modified electrode rGO / ITO:

[0034]The ITO conductive glass was immersed in acetone, ethanol and ultrapure water for 30 min, and the high-purity nitrogen was purged and dried for later use; the ITO conductive glass was immersed in 0.2 mg / mL graphene oxide (GO) aqueous solution, and deposited by constant current method, the current density is 0.25 mA / cm 2 , the deposition time was 100 s, and the electrode rGO / ITO with uniform loading of reduced graphene oxide (rGO) was prepared;

[0035] (1.2) Preparation of modified electrode ZnO-rGO / ITO:

[0036] Immerse rGO / ITO in 0.02 mol / L Zn(NO 3 ) 2 The modified electrode ZnO-rGO / ITO with uniform loading of ZnO nanorods was prepared by constant potential depositio...

Embodiment 2

[0049] Monitoring the assembly process of aptasensor by current-time curve method

[0050] Figure 6 In order to adopt a four-electrode detection system, the photoelectric response curves of different modified electrodes were tested with a phosphate buffer solution containing 0.1 mol / L DA as the electrolyte. Curves a to e represent the modified electrodes ZnO-rGO / ITO, ZnO-rGO / ITO, Photoelectric response curves of Au / ZnO-rGO / ITO, S1 / Au / ZnO-rGO / ITO, S2 / S1 / Au / ZnO-rGO / ITO and MB / S2 / S1 / Au / ZnO-rGO / ITO, ZnO- After rGO / ITO deposits Au nanoparticles (Au / ZnO-rGO / ITO), the photocurrent increases significantly, and the good conductivity and local surface plasmon resonance effect of Au nanoparticles promote the separation of photogenerated electron-hole pairs. and electron transfer. When Au / ZnO-rGO / ITO surface self-assembled aptamer S1 (S1 / Au / ZnO-rGO / ITO), its photocurrent decreased significantly, and the negatively charged oligonucleotide sequence hindered the transfer of electrons, At...

Embodiment 3

[0052] An application of a photoelectrochemical aptasensor using nano-gold / zinc oxide-reduced graphene oxide ternary composite material as a photosensitive element, comprising the following steps:

[0053] 20 μL of different concentrations of Cd(II) solutions were incubated on the surface of the photoelectrochemical aptasensor MB / S2 / S1 / Au / ZnO-rGO / ITO in Example 1, and incubated at room temperature and humidity for 1 h. A four-electrode detection system was used to test the photocurrent of the sensor after incubation with Cd(II), and a linear regression equation was constructed according to the relationship between the concentration of Cd(II) and the photocurrent response. Figure 7 (A) is the incubation concentration of sensors MB / S2 / S1 / Au / ZnO-rGO / ITO in Example 1 of the present invention is 5.0×10 -12 , 6.0×10 -11 , 3.0×10 -10 , 1.0×10 -9 , 6.0×10 -9 , 2.0×10 -8 Photocurrent response curves after Cd(II) in mol / L (curves a to f), (B) is the linear regression graph of Cd(I...

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Abstract

The invention relates to construction and application of a photoelectrochemical aptamer sensor taking a nanogold / zinc oxide-reduced graphene oxide ternary composite material as a photoelectric sensitive element. The construction comprises the following steps: by taking conductive glass (ITO) as a substrate electrode, preparing a photoelectrode Au / ZnO-rGO / ITO modified by reduced graphene oxide (rGO), zinc oxide (ZnO) and nanogold (Au), and modifying an aptamer S1 and a complementary strand S2 to form double-stranded DNA (dsDNA); and combining photosensitizer methylene blue (MB) with the dsDNA to construct a sensor for amplifying a detection signal. After a sensing interface senses Cd (II), the S2 specifically recognizes the Cd (II) to form a hairpin structure, and along with dsDNA unwindingand MB separation from the dsDNA, quantitative analysis of the Cd (II) is realized according to the photocurrent response of the recognized sensor. A double-working-electrode method is adopted for testing, a sensor and a glassy carbon electrode serve as a first working electrode and a second working electrode, dopamine (DA) is added into an electrolyte to serve as an electron donor, oxidation-reduction circulation of DA on the surfaces of the double working electrodes is achieved, and signal stability is improved. The method has the advantages of high sensitivity and low detection limit.

Description

technical field [0001] The invention relates to the technical field of functional nanocomposite materials and biosensing analysis, and provides a construction method and application of a photoelectrochemical aptamer sensor using a nano-gold / zinc oxide-reduced graphene oxide ternary composite material as a photosensitive element . Background technique [0002] Cadmium ion Cd(II) is a heavy metal ion with strong biological toxicity, which mainly comes from metal mine mining, smelting, electrolysis, pesticide, medicine, paint, textile printing and dyeing and other industries, which not only causes serious damage to the local ecological environment It will be destroyed, and will enter the human body along with the food chain to replace the calcium in the bones, causing osteoporosis, osteomalacia and other diseases, making people feel bone pain, fatigue, headache, dizziness, etc., and it will also be rich in liver and kidneys. Accumulation, resulting in cumulative poisoning and ...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48G01N27/30
CPCG01N27/3278G01N27/48G01N27/305
Inventor 孙伟牛燕燕罗贵铃谢慧张晓萍
Owner HAINAN NORMAL UNIV
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