Application of two isoflavone compounds in prevention and treatment of plant diseases

A technology for plant diseases and compounds, applied in the directions of plant growth regulators, botanical equipment and methods, applications, etc., can solve problems such as reports on the use of isoflavones

Inactive Publication Date: 2020-09-29
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of the above two isoflavones in anti-phytopathogenic bacteria

Method used

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  • Application of two isoflavone compounds in prevention and treatment of plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take 5 kg of dried licorice root, grind it and extract it under reflux with 80% ethanol for 3 times. Dry to obtain 200 g of ethyl acetate partial extract. The extract was subjected to silica gel column chromatography, and gradient elution was carried out with petroleum ether-ethyl acetate system (100:0-0:100). The components eluted from petroleum ether-ethyl acetate (50:1) were separated and purified by silica gel column chromatography and methanol recrystallization repeatedly to obtain the monomer compound 4'- O - Methylglabridin (21mg) and Oglycyrrhizin B (16mg).

Embodiment 2

[0016] Isoflavone suspending agent with active ingredient concentration of 20%, the prescription composition takes 100g sample as an example: isoflavone compound 20g, dispersant sodium methylene dinaphthalene sulfonate 5g, wetting agent sodium lignin sulfate 3g, stabilizer benzene Formic acid 0.5g, antifreeze agent ethylene glycol 6g, defoamer tributyl phosphate 2g, preservative potassium sorbate 0.5g, thickener xanthan gum 0.5g and soft water 62.5g.

[0017] Preparation process: Put the measured amount of isoflavones, sodium methylene dinaphthalene sulfonate, sodium lignin sulfate, benzoic acid, ethylene glycol, potassium sorbate, and soft water into the shearing machine for 30 minutes; use sand Grind the sheared material 3 times with a mill, and add a metered amount of tributyl phosphate while grinding; add xanthan gum to the ground material and shear it for 20 minutes, and pack it after passing the test.

Embodiment 3

[0019] Isoflavone wettable powder with an active ingredient concentration of 25%, the prescription composition takes a 100g sample as an example: 25g of isoflavone compounds, 3g of dispersant BX pull-off powder, 0.5g of suspending agent hydroxypropyl methylcellulose, wetting Agent sodium dodecylbenzene sulfonate 3g and carrier kaolin 68.5g.

[0020] Preparation process: Mix measured amounts of BX powder, hydroxypropyl methylcellulose and sodium dodecylbenzenesulfonate, grind and pulverize, add measured amounts of isoflavone compounds to obtain active ingredient powder, and then add measured amounts The kaolin is mixed uniformly and passed through a 250-mesh sieve to obtain a wettable powder.

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Abstract

The invention relates to application of two isoflavone compounds in prevention and treatment of plant diseases, belonging to the technical field of botanical bactericides. The compounds are 4'-O-methyl glabridin and glycyrrhizin B respectively, can be separated and extracted from glycyrrhiza plants, and can also be prepared through a chemical synthesis or biosynthesis method. The compounds can effectively inhibit sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea, Chinese wolfberry colletotrichum gloeosporioides, rhizoctonia solani, fusarium oxysporum, magnaporthe grisea and xanthomonas oryzae pv. Oryzae, and botanical bactericides prepared by using the compounds as active components can be used for preventing and treating plant diseases caused by the above pathogens. The technical scheme provided by the invention has the characteristics of environmental friendliness, relative safety to non-target organisms and the like, and can relieve the problem of drug resistance whenbeing alternately used with existing bactericides.

Description

technical field [0001] The invention belongs to the technical field of botanical fungicides, and in particular relates to the use of two isoflavone compounds in preventing and treating plant diseases. Background technique [0002] Plant diseases are a serious hazard in agricultural production and cause huge economic losses around the world every year. Chemical control is an important means to prevent and control plant diseases. However, the long-term use of chemical pesticides has caused serious environmental problems and resistance problems. Therefore, it is urgent to develop new green control agents for alternate use. [0003] 4'- O -Methyl glabridin and licoricein B belong to isoflavone compounds, mainly from the leguminosae (Leguminosae) licorice ( Glycyrrhiza Linn.) isolated from plants. At present, the use of the above two isoflavones mainly focuses on the treatment of diabetes, hyperlipidemia, hypertension (Chinese patent CN1777420A, world patent WO2005011672A1), ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P3/00
CPCA01N43/90
Inventor 赵兴增徐曙李林蔚周倩陈雨冯煦郭生虎
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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