bacteroides polymorphus GL-02, a complex microbial inoculant compounded by the bacteroides polymorphus GL-02 and bile acid and application of the complex microbial inoculant

A technology of Bacteroides polymorpha and GL-02, applied in the field of microorganisms, can solve the problems of fat metabolism disorder, early calf development, liver disease, etc., to promote absorption, facilitate breeding and development, improve material metabolism and Effects of hepatic metabolism

Active Publication Date: 2021-01-29
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0004] During the lactation period of dairy cows, due to the regulatory effects of progesterone, estrogen and other reproductive hormones or leptin and neuropeptide Y, the energy of the cows will be in a negative balance state. At this stage, cows need to mobilize body fat to meet their own needs energy needs: a large amount of non-esterified fatty acids (NEFA) will be released from adipose tissue and enter the liver for metabolism, some of which are oxidized in the liver to provide energy, and some of them are re-deposited in the form of triglycerides (TG) In liver cells, but the lack of lipoprotein lipase and hepatic lipase in the liver of dairy cows will limit the catabolism of TG, easily cause fat metabolism disorders, and cause liver diseases at the same time, and liver diseases will affect other metabolic functions, health and milk production of dairy cows will have varying degrees of adverse effects
[0005] In addition, in order to reduce the cost of feeding, the calves produced by dairy cows often adopt the early weaning method, and the milk in the early stage is artificially fed, which also leads to the occurrence of early stunted calves

Method used

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  • bacteroides polymorphus GL-02, a complex microbial inoculant compounded by the bacteroides polymorphus GL-02 and bile acid and application of the complex microbial inoculant
  • bacteroides polymorphus GL-02, a complex microbial inoculant compounded by the bacteroides polymorphus GL-02 and bile acid and application of the complex microbial inoculant
  • bacteroides polymorphus GL-02, a complex microbial inoculant compounded by the bacteroides polymorphus GL-02 and bile acid and application of the complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Screening and Identification of Bacteroides polymorpha GL-02

[0026] Take 10g of healthy pig feces and 100mL of sterile water and mix them evenly, filter and remove impurities to make a feces dilution, and then continue to add sterile water to the dilution to prepare 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 diluent; then take 10 -2 、10 -3 、10 -4 、10 -5 Spread the dilution on GAM agar medium containing 80% N 2 , 10% CO 2 and 10%H 2 Under the anaerobic environment, cultivated at 35°C for 48 hours, selected obvious colonies and purified them several times to obtain a single colony, which was named GL-02 and stored in a frozen state. Among them, the formula of GAM agar medium is: 10.0g peptone, 3.0g soytone, 10.0g monthly peptone, 13.5g serum digestion powder, 2.2g beef extract powder, 5.0g yeast extract powder, 1.2g beef liver extract powder, 3.0 g glucose, 3.0g sodium chloride, 2.5g potassium dihydrogen phosphate, 5.0g soluble starch, 0.3g L-cysteine, 0.15...

Embodiment 2

[0029] Embodiment 2: the preparation of composite bacterial agent

[0030] 1. Preparation of bile acids

[0031] The preparation process steps of described bile acid are as follows:

[0032] (1) Saponification: Add 1 kg of pig gall powder crushed through a 40-mesh sieve to the reaction kettle, then add 10L of sodium hydroxide solution with a mass concentration of 10%, heat and stir until boiling, and keep stirring for 14 hours in a boiling state, and then react The generated saponified liquid is cooled to solid-liquid separation, the supernatant is removed, and the remaining solid is bile acid saponified product;

[0033] (2) Decolorization: Add 6L of water to the bile acid saponification product, heat to 80°C to completely dissolve the bile acid saponification product, then pour it into a decolorization tank, add 300mL hydrogen peroxide and stir evenly, react at room temperature for 24 hours, and then put the reaction The solution is filtered into the acidification tank to ...

Embodiment 3

[0044] A dairy farm in Shandong has 800 dairy cows, and 20 dairy cows are randomly selected, including 10 cows in the experimental group and 10 in the control group. The selected cows had similar physiological status and were in lactation 30 days after parturition. Experimental group: the compound bacterial agent prepared in Example 2 was added to the full mixed ration of dairy cows with an addition amount of 60 g / day / head, and fed continuously for 30 days, and the dosage of the compound bacterial agent in the morning and evening was doubled ; Control group: The dairy cows were fed with the basic total mixed ration for 30 consecutive days; the feeding management conditions of the two groups of dairy cows were the same, and both were carried out according to the daily breeding management.

[0045] During the experiment, the clinical status, culling situation and milking volume of the two groups of dairy cows were recorded. The results are shown in Table 1 and Table 2. It can be...

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Abstract

The invention discloses bacteroides polymorphus GL-02, a complex microbial inoculant compounded by the bacteroides polymorphus GL-02 and bile acid and application of the complex microbial inoculant. The classification name of the bacteroides polymorphus GL-02 is bacteroides polymorphus thetaotomicron, the bacteroides polymorphus GL-02 is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the bacteroides polymorphus GL02 is CGMCC No.20818. The bacterial colony of the strain is circular or quasi-circular, yellow, smooth and lusterless in surface, slightly convex in middle, opaque and neat in edge, and the diameter of the bacterial colony is 0.5-2.5 mm. The invention further provides a complex microbial inoculant prepared from the bacteroides polymorphus GL-02 bacterial powder and bile acid. The complex microbial inoculant can increase the milk yield and survival rate of cows in the lactation period, recover and can improve the metabolic function, material metabolism and liver function of the cows and promote growth of the calves of the cows.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Bacteroides polymorpha GL-02 and its compound bacterial agent compounded with bile acids and its application. [0002] technical background [0003] Because milk is rich in nutrients such as protein, fat, vitamins and minerals, it is a drink suitable for long-term drinking, so the large-scale breeding of dairy cows in my country is increasing year by year. [0004] During the lactation period of dairy cows, due to the regulatory effects of progesterone, estrogen and other reproductive hormones or leptin and neuropeptide Y, the energy of the cows will be in a negative balance state. At this stage, cows need to mobilize body fat to meet their own needs energy needs: a large amount of non-esterified fatty acids (NEFA) will be released from adipose tissue and enter the liver for metabolism, some of which are oxidized in the liver to provide energy, and some of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K20/168A23K50/10A23K50/60C12R1/01
CPCA23K10/18A23K20/168A23K50/10A23K50/60C12N1/20C12N1/205C12R2001/01
Inventor 籍立民张西雷娄倩倩
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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