Asexual rapid propagation method for dicotyledons

Abstract

The invention provides an asexual rapid propagation method for dicotyledons. The method comprises the following steps: 1) cutting leaves with complete axillary buds from new branches of dicotyledon plants in the current year; 2) removing xylem tissues at the basal part of the petiole, then putting the leaves into sterile water for cleaning, and taking the trimmed leaves as explants; 3) spraying arooting powder solution to the leaf surfaces of the explants once, and then putting the leaf explants into a seed germination box for water culture until petioles root; 4) transplanting the rooted leaf explant into soil for cultivation. According to the method, the leaf explants are utilized for the first time to establish a method for rapidly propagating and nursing dicotyledon (such as tea trees, peanuts and cotton) materials, and the method is reliable and stable. Dicotyledon plant leaves for propagation are sufficient in source, few materials are needed for experiments, the survival rate of new plants is high, the experiment period is short, and the method has the potential of factory-like large-scale production. The method has great application value in the fields of rapid propagationand popularization of excellent varieties of tea trees, preservation of specific materials and the like. The method also has a certain reference value for gymnospermae and monocotyledons.

Description

technical field [0001] The invention relates to the field of plant asexual propagation, in particular to a method for rapid asexual propagation of dicotyledonous plants. Background technique [0002] Tea is one of the most important beverage crops in the world. With the improvement of people's living standards, the demand for tea is increasing day by day, and the contradiction between supply and demand of high-quality tea seedlings is becoming increasingly prominent. Tea tree reproduction is divided into two methods: sexual reproduction and asexual reproduction. Tea tree seed (sexual) reproduction refers to the method of producing seeds through the combination of male and female gametes of tea trees, and using tea seeds to sow and raise seedlings to reproduce offspring, also known as seed reproduction. The vegetative propagation of tea trees is also called vegetative propagation. It uses the roots, stems and other vegetative organs of tea trees to form a new tea seedling un...

AI Technical Summary

Problems solved by technology

[0003] Yet, no matter be sexual reproduction method or asexual reproduction method, all there is more shortcoming

Defects of sexual reproduction method: 1. Because the seeds have the genetic characteristics of both parents, the offspring of the seeds are often of poor purity, with large differences in plant growth and different growth periods, which is not easy to manage

2. The raw materials of fresh leaves are uneven in thickness and tenderness, which is not conducive to the mastery of processing technology and the production and development of famous tea

3. For high-quality tea tree varieties with low dry seed setting rate or weak root wood, it is difficult to propagate by seeds

Defects of asexual propagation method: 1. A large number of pruning and pruning have a greater impact on the growth of the mother tree and the yield of tea; 2. The requirements for breeding and planting technology are high, labor consumption is high, and the cost is high; 3. The adaptability of tea seedlings is higher than that of seedlings Difference

[0004] Due to the increasing demand for high-quality tea seedlings, there are many shortcomings in existing tea tree propagation methods. Therefore, it is urgent to establish a new type of tea tree propagation technology to achieve fast, effective, convenient and economical purposes.

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