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Lactobacillus fermentum MF423, fermented rice bran extract thereof and application of lactobacillus fermentum MF423 and fermented rice bran extract

A technology of fermenting Lactobacillus and rice bran extract, which is applied in the field of microorganisms, can solve the problems of low utilization rate of rice bran, achieve the effects of reducing glucose content, inhibiting the occurrence of low-grade inflammation, and improving insulin resistance

Active Publication Date: 2021-06-11
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been some studies at home and abroad that fermenting rice bran can improve its nutritional value, but no research has found that using lactic acid bacteria to ferment rice bran can improve its nutritional value, and overall, the utilization rate of rice bran is still low

Method used

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  • Lactobacillus fermentum MF423, fermented rice bran extract thereof and application of lactobacillus fermentum MF423 and fermented rice bran extract
  • Lactobacillus fermentum MF423, fermented rice bran extract thereof and application of lactobacillus fermentum MF423 and fermented rice bran extract
  • Lactobacillus fermentum MF423, fermented rice bran extract thereof and application of lactobacillus fermentum MF423 and fermented rice bran extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Separation of Lactobacillus fermentum (Lactobacillus fermentum) MF423 Sample processing: draw 500 μL of rice flour fermentation waste water stored at 4°C, draw 50 μL, 100 μL, and 200 μL respectively and spread them on the MRS solid medium plate. Repeat three times, and then place them in a 37°C incubator for static culture. After each colony grows well, streak the plate repeatedly, separate and purify, and finally obtain strain MF423.

Embodiment 2

[0062] Example 2 Bacterial Strain MF423 Biological Characters and Physiological Biochemical and Molecular Identification

[0063] S1. Morphological characteristics: The morphology of the strain MF423 under the scanning electron microscope is shown in figure 1 , The colony morphology of the strain was cultured on MRS solid medium plate for 24 hours: the colony was white, round, with smooth surface and neat edges; Gram staining was positive, and the cells were rod-shaped.

[0064] S2. Physiological and biochemical characteristics: bacterial strain MF423 of the present invention is measured through physiological and biochemical, and contact enzyme test is positive, and methyl red test is negative, and V-P test is negative, and starch hydrolysis, agar hydrolysis, Tween 80 hydrolysis are negative, and growth temperature is 4-30°C.

[0065] See Table 1.

[0066] Table 1 Physiological and biochemical characteristics of Lactobacillus fermentum MF423

[0067]

[0068]

[0069]...

Embodiment 3 3

[0081] Example 3 In vitro antioxidant activity of three different Lactobacillus fermented rice bran extracts

[0082] Inoculate the three strains of Lactobacillus in MRS liquid culture medium respectively, culture at 37°C for 24 hours, inoculate the fermentation medium at 2% respectively, culture at 37°C, take the fermentation liquid fermented for 24 hours, and centrifuge twice at 10000rpm to remove bacteria and Rice bran residue, take the upper layer of fermentation broth to measure the hydroxyl radical scavenging activity: add 40 μL Fe to a clean EP tube 2+ , then add 40μL OP, mix well, the solution turns into an orange-red complex, and add to the experimental group (A s ) and the negative control group (A n ) was added with 80 μL sample, the blank control group (A b ) add 80μL dd H 2 O, after mixing evenly, start the reaction, the experimental group (A s ) by adding 40 μL H 2 o 2 , negative control group (A n ) by adding 40 μL H 2 O, blank control group (A b ) by a...

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PUM

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Abstract

The invention relates to the technical field of microorganisms, in particular to lactobacillus fermentum MF423 from rice flour fermentation wastewater and application of the lactobacillus fermentum MF423. The lactobacillus fermentum MF423 fermented rice bran extract disclosed by the invention has an effect of improving insulin resistance in vivo and in vitro. Through determination, on the cell and animal level, the rice bran extract fermented by the bacterium has relatively high antioxidant activity, and can be used for remarkably increasing the consumption of glucose in a culture medium by cells, reducing the blood sugar of mice, remarkably eliminating lipid accumulation of the mice and inhibiting the occurrence of low-grade inflammation, and has important value in subsequent efficient utilization of low-value resource rice bran and application of the rice bran in clinical treatment of the type 2 diabetes mellitus.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Lactobacillus fermentum MF423 and its fermented rice bran extract and their application. Background technique [0002] Lactic acid bacteria (LAB) is a kind of Gram-positive and catalase-negative bacteria that produce lactic acid. It is widely distributed and has rich species diversity. There are more than 200 species, and most of them have various physiological Function. Lactic acid bacteria are widely distributed in nature, and can be isolated from plant surfaces, dairy products, meat products, beer, wine, fruit juice, wort, fermented dough, sewage, and human and animal manure. In addition to the various probiotic effects of lactic acid bacteria themselves, they can also produce a variety of functional active ingredients during the food fermentation process. In addition to obtaining a variety of active substances that are beneficial to health, lactic acid ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04A23L33/10A23L7/10A23L7/104C12R1/225
CPCC12P1/04A23L33/10A23L7/115A23L7/104A23V2002/00A23V2400/143A23V2200/30A23V2200/328
Inventor 何海伦艾晓娟殷婷婷颜晓涛饶海潋蒋利玲张好
Owner CENT SOUTH UNIV
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