Novel composite hydrogel stent prepared by 3D biological printing technology and application of novel composite hydrogel stent

Abstract

The invention discloses a novel composite hydrogel stent prepared by a 3D biological printing technology and application of the novel composite hydrogel stent. According to the invention, oxidized sodium alginate and sodium alginate are used as substrates, gelatin and calcium chloride are used as cross-linking agents, and calcium carbonate particles are used as a stabilizer. The composite stent is rough in surface, beneficial to cell adhesion, provided with a communicated hole structure uniform in size, capable of remarkably promoting the wound healing effect and good in degradation rate, and the degradation rate can be matched with the tissue regeneration rate. In the early stage of wound healing, the stent quickly absorbs tissue fluid, covers and fills the wound, and provides a microenvironment similar to an in-vivo extracellular matrix for cells; the porous supporting structure induces tissue cells to proliferate in parallel in the stent, collagen deposition is accelerated, and a complete nutrient supply platform is constructed. In the later stage of wound healing, since the scaffold contains oxidized sodium alginate, the degradation process of the oxidized sodium alginate promotes subsequent deposition of skin tissues in the scaffold and proliferation of tissue cells, the healing speed is increased, and scar formation is reduced.

Description

technical field [0001] The present invention relates to the field of 3D bioprinting technology and the field of wound healing, in particular to a novel composite hydrogel scaffold prepared by 3D bioprinting technology and its application. Background technique [0002] The incidence of diabetes mellitus (DM) is high, and diabetic foot ulcer (DFU) is one of its most important complications. The wounds are often hindered at the beginning of healing, and gradually turn into chronic non-healing wounds. If not treated in time, it may lead to amputation and death. For diabetic wounds, a variety of new wet dressings are often selected clinically for segmental treatment of wounds, such as the combination of film dressings, alginate dressings and hydrogel dressings. If the skin loss is severe, artificial skin grafting is also required. Although the above treatment methods have achieved certain effects, the skin substitutes are expensive. At present, there are dressings to promote wou...

AI Technical Summary

Problems solved by technology

Diabetic wounds also have hypoxia, abnormal angiogenesis, extracellular matrix deposition and remodeling blockage, resulting in slow or non-healing wounds

However, most of the current hydrogel scaffolds have a sheet structure, poor adhesion to the wound, poor biodegradability, poor mechanical properties, and less internal microporous structure, which cannot provide cells with a microenvironment similar to that of the ECM in vivo. , Insufficient supply of nutrients, limited cell proliferation and tissue regeneration, resulting in weak integration with wound tissue and failed to significantly promote wound healing

The most commonly used flaky hydrogel dressings on the market (such as Dongweigao and Danish Coloplast hydrogel dressings) are single-layer film structures as shown in Figure 1. Although the water content is large, it can maintain a moist environment on the wound surface, but the dressing It does not have an internal microporous three-dimensional structure, which is not conducive to the transmission of nutrients, and is not degradable in the body, so it cannot ensure that the dressing fits closely with the wound surface. It can only be used for autolytic debridement, and its function is relatively single

Images