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Trichoderma brevicompactum GSAAMLSHU-1 water dispersible granule as well as preparation method and application thereof

A technology of Trichoderma brevis and dispersing particles, which is applied in the directions of botanical equipment and methods, biochemical equipment and methods, and applications, can solve the problems of low control effect stability, poor sporulation activity, and short shelf life, etc. Achieve the effect of increasing utilization value, high content of active ingredients, and preventing root rot and stem rot

Inactive Publication Date: 2021-10-22
甘肃省农业科学院植物保护研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the Trichoderma preparations on the market have a short shelf life and poor sporulation activity, which can easily lead to low stability of their control effects in the field, and are also easily disturbed by various factors such as light and temperature during transportation and storage.

Method used

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  • Trichoderma brevicompactum GSAAMLSHU-1 water dispersible granule as well as preparation method and application thereof
  • Trichoderma brevicompactum GSAAMLSHU-1 water dispersible granule as well as preparation method and application thereof
  • Trichoderma brevicompactum GSAAMLSHU-1 water dispersible granule as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0046] 1.3.1 Preparation of liquid strains

[0047] Take 75mL of the prepared liquid culture medium in a 150mL Erlenmeyer flask, sterilize at 121°C for 30min, and insert 1mL of 10 7 Cfu / g Trichoderma brevis GSAAMLSHU-1 spore suspension was cultured on a shaker at 25°C and 140r / min for 5 days, and set aside.

[0048] The preparation method of described liquid culture medium is as follows:

[0049] Weigh the following raw materials: 200g of peeled potatoes, 20g of glucose and 1000mL of distilled water; cut the peeled potatoes into small pieces and put them in the pot, add distilled water, boil for 30min, after cooling slightly, filter the potatoes with four layers of gauze, pour the filtrate into Add glucose to the measuring cup, after fully dissolved, add water to make up to 1000mL. Then it was divided into Erlenmeyer flasks and sterilized at 121°C for 30 minutes.

[0050] 1.3.2 Preparation of solid strains

[0051] Put the solid matrix of wheat bran: corn stalk = 3:1 (mass...

Embodiment 2

[0101] The stability of embodiment 2 water-dispersible granules

[0102] The Trichoderma brevis GSAAMLSHU-1 water-dispersible granule prepared under the best auxiliary agent formula of Example 1 is stored under refrigeration (4 ± 2°C) and room temperature (25°C) for 300d. The change of the spore germination rate is as follows: Figure 8 shown. The results showed that the spore germination rate of Trichoderma brevistum GSAAMLSHU-1 water-dispersible granules stored under refrigeration was higher than that under room temperature. The preparation was stored under refrigeration (4±2°C) for 300 days, and the spore germination rate was 48.1%, while the spore germination rate at room temperature (25°C) was 31.0%. It can be seen that low temperature storage is more conducive to the extension of shelf life.

Embodiment 3

[0103] Embodiment 3 Water-dispersible granules are applied to the prevention and treatment of plant diseases

[0104] The present embodiment shows that Trichoderma brevis GSAAMLSHU-1 water-dispersible granules (the Trichoderma brevis GSAAMLSHU-1 water-dispersible granules prepared under the optimum adjuvant formula of embodiment 1) has the effect on the roots of corn seedlings by pot experiments. Rot prevention effect.

[0105] 1. Test method: select corn seeds (integrity 16) of uniform size, sterilize with 70% alcohol for 5 minutes, and wash them with sterile water for 3 times. 1 kg of soil per pot, sow 6 seeds. Seedlings with uniform growth were selected after germination. Plan the root soil before inoculation, cut the root with a scalpel, and inoculate the wound with an area of ​​50mm 2 After the pathogenic bacteria block, cover with soil. After 5 days, mix 5 g of water-dispersible granules with 495 mL of water, and irrigate the roots with 10 mL of the prepared biocontr...

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Abstract

The invention provides a trichoderma brevicompactum GSAAMLSHU-1 water dispersible granule as well as a preparation method and application of the trichoderma brevicompactum GSAAMLSHU-1 water dispersible granule. The optimal formula of the water dispersible granule is as follows: trichoderma brevicompactum GSAAMLSHU-1 parent drug, ascorbic acid accounting for 0.5% of the mass of the parent drug, calcium carbonate accounting for 4% of the mass of the parent drug, attapulgite accounting for 5% of the mass of the parent drug, soluble starch accounting for 5% of the mass of the parent drug, sodium dodecyl benzene sulfonate accounting for 5% of the mass of the parent drug and polyvinyl pyrrolidone accounting for 5% of the mass of the parent drug. The water content of the water dispersible granules is 33%-37%; the content of trichoderma brevicompactum spores is 2.9 * 10 < 8 > cfu / g to 5.1 * 10 < 8 > cfu / g. The trichoderma brevicompactum GSAAMLSHU-1 water dispersible granule provided by the invention is high in content of effective components, the preservation time of a fungicide can be prolonged, the use efficiency can be improved, the utilization value of trichoderma can be increased, and root rot and stem rot in a corn seedling stage can be effectively prevented and treated.

Description

technical field [0001] The invention relates to the field of biopesticides, in particular to a water-dispersible granule of Trichoderma brevisdense GSAAMLSHU-1 and a preparation method and application thereof. Background technique [0002] my country is a large agricultural country, and agriculture is the foundation of the national economy. Pesticides are important means of agricultural production, and are crucial to disease prevention and pest control, and the promotion of stable and high yields of grain. However, due to the long-term excessive use of chemical pesticides, the resistance of diseases and insect pests has increased, and the control effect has decreased. Therefore, biocontrol agents stand out with their advantages of low risk and good environmental compatibility. As a biocontrol fungus with strong adaptability, broad antibacterial spectrum and diverse antagonistic mechanisms, Trichoderma Pers. is widely distributed and exists in nature. Because of its simple...

Claims

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Application Information

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IPC IPC(8): A01N25/14A01N63/38A01N25/08A01N25/22A01P3/00A01P21/00C12N1/14C05G3/00C05G3/60C12R1/885
CPCA01N25/14A01N63/38A01N25/08A01N25/22C12N1/14C05G3/00C05G3/60Y02A40/10
Inventor 郭成张小杰洪流景芳王春明周天旺
Owner 甘肃省农业科学院植物保护研究所
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