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Bifidobacterium longum capable of reducing inflammatory response and relieving constipation

A technology of Bifidobacterium longum and bacteria agent, applied in the field of microbiology, can solve the problems of laxative dependence and other problems, and achieve the effect of avoiding side effects, obvious effect and good growth characteristics

Active Publication Date: 2022-01-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For patients with constipation, they are often encouraged to eat more foods rich in dietary fiber, such as fresh vegetables, fruits, and beans; osmotic or stimulant laxatives, such as polyethylene glycol, lactulose, or anthraquinone derivatives, can also be used, but Such laxatives often cause dependence, and even side effects such as nausea, abdominal pain, and diarrhea

Method used

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  • Bifidobacterium longum capable of reducing inflammatory response and relieving constipation
  • Bifidobacterium longum capable of reducing inflammatory response and relieving constipation
  • Bifidobacterium longum capable of reducing inflammatory response and relieving constipation

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0073] (4) Preparation of Bifidobacterium longum suspension

[0074] Inoculate the 3-generation activated bacterial solution into 1L of liquid MRS medium at an inoculum size of 2%, shake and mix well, and incubate in an anaerobic incubator at 37°C for 24h. Centrifuge at 8000g / min, 4°C for 15min, remove the supernatant, wash twice with sterile normal saline (containing 0.05%-0.1% L-cysteine ​​hydrochloride), also under the same conditions After centrifugation, the supernatant was removed, stored in 30% glycerol, and stored in a -80°C refrigerator for one week. Before the animal experiment, centrifuge the bacterial solution at 6000r / min for 5min, wash it twice with sterile saline, resuspend the bacterial solution with 10% skimmed milk, shake it evenly, and use the plate pouring method to measure the initial and one week after freezing the number of live bacteria. The formula of MRS medium is: beef extract 10g; tryptone 10g; yeast powder 5g; glucose 20g; anhydrous sodium acetat...

Embodiment 1

[0076] Example 1: Relief of constipation-related symptoms induced by loperamide by Bifidobacterium longum CCFM1112

[0077] Bifidobacterium longum CCFM1112, Bifidobacterium bifidum CGMCC NO.13632 (the strain is disclosed in the patent document CN106834187B) and Bifidobacterium longum CCFM642 (the strain is disclosed in the paper DOI: 10.3389 / fmicb.2019.01721 Middle) strains were taken out from the -80°C refrigerator, streaked on the MRS plate, cultured at 37°C for 48 hours, picked a single colony in the MRS liquid tube, cultured at 37°C for 24 hours, and inoculated on a new MRS at a volume of 2%. In liquid culture medium, cultivate at 37°C for 24 hours, culture for another generation in the same way, then centrifuge the bifidobacterium suspension at 6000r / min, 4°C for 5min, and then resuspend with 10% skimmed milk, Bacterial suspensions were obtained for animal experiments.

[0078] Thirty 6-week-old healthy male Balb / c mice were taken, adapted to the environment for 1 week, ...

Embodiment 2

[0088] Embodiment 2: Bifidobacterium longum CCFM1112 can reduce the content of somatostatin (SS) in the serum of constipation mice

[0089]Balb / c mice grouping, modeling and treatment methods were the same as in Example 1. After the mice were killed on the 39th day, the collected mouse blood was left to stand for 2 hours, and the serum was obtained after centrifugation at 3000g for 15 minutes. The somatostatin (SS) detection kit was used to perform experiments according to the instructions, and the SS in the serum was calculated from the standard curve. concentration.

[0090] Studies have shown that somatostatin has a certain inhibitory effect on gastrointestinal motility and secretion of digestive tract hormones. Experimental results such as figure 2 shown by figure 2 It can be seen that compared with the model group, Bifidobacterium longum CCFM1112 group can significantly down-regulate the serum SS content of constipated mice, while the serum SS content of mice treated...

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Abstract

The invention discloses bifidobacterium longum capable of reducing inflammatory response and relieving constipation, and belongs to the field of microorganisms. The bifidobacterium longum CCFM1112 provided by the invention can effectively reduce the whole body metabolic circulation and the inflammatory response of the colon part, and improve the first black stool time, the intestinal tract propulsion rate, the water content of the stool and the pathological damage of the colon tissue of a constipation mouse. For physiological indexes, the strain can influence the content of excitement type and inhibition type gastrointestinal active peptides, the content of four short-chain fatty acids is increased, and the symptom of constipation is relieved fundamentally. The bifidobacterium longum not only has a certain considerable effect in physiological and pathological aspects and has no side effects compared with laxative, but also can improve the bioavailability of rutin in a body, so that the rutin can better exert the anti-inflammatory and anti-oxidation effects, therefore, the bifidobacterium longum strain provided by the invention can be widely added into various foods or medicines, so that the bifidobacterium longum strain has better application value.

Description

technical field [0001] The invention relates to a strain of Bifidobacterium longum which reduces inflammatory response and relieves constipation, and belongs to the field of microorganisms. Background technique [0002] Constipation is a common gastrointestinal disorder worldwide with a prevalence of up to 15%. In general, the prevalence rate of non-whites is higher than that of whites. Secondly, the male to female ratio is roughly 1:1.5, but this disease is not only for all ages, races, ethnic groups, or for different socioeconomic groups It is a very widespread disease. [0003] The main symptoms of constipation are infrequent bowel movements, less than 3 times a week, straining stools, a feeling of endless defecation, the need for fingers to assist defecation, abdominal distension, and dry, hard, and lumpy stools. There are many classifications of constipation. Constipation can be divided into three types: normal transit constipation, slow transit constipation, and pelv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/10A61P1/00A23L33/00A23C9/12A23C9/123A23C13/16A23C19/00A23L11/50A23L11/65C12R1/01
CPCC12N1/20A61K35/745A61P1/10A61P1/00A23L33/00A23C9/1203A23C9/123A23C13/16A23C19/00A23C11/106A23V2002/00A23V2400/533A23V2200/30A23V2200/32Y02A50/30
Inventor 王刚陈卫柴茂赵建新张灏
Owner JIANGNAN UNIV
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