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Preparation method and application of monodisperse magnetic polydopamine microspheres

A polydopamine microsphere, monodispersity technology, applied in the preparation method of peptide, microsphere preparation, microcapsule preparation, etc., can solve the problem of uneven particle size distribution of magnetic microspheres, easy agglomeration of magnetic microspheres, surface There are few problems such as few functional groups, so as to achieve the effect of enhanced extraction effect, fast magnetic response, and good dispersion.

Pending Publication Date: 2022-03-01
南通伯仲纳米科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, polymer magnetic microspheres mostly adopt dispersion polymerization method, seed polymerization method, suspension polymerization method, etc. Most of the magnetic microspheres prepared by these methods have disadvantages such as uneven particle size distribution, low magnetic content, and few surface functional groups.
Among them, the magnetic microspheres prepared by the seed polymerization method are easy to agglomerate with each other, which causes a decrease in the relative area and a decrease in the functional groups available for adsorption per unit area, resulting in large measurement errors, high background values, and low sensitivity. question

Method used

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  • Preparation method and application of monodisperse magnetic polydopamine microspheres
  • Preparation method and application of monodisperse magnetic polydopamine microspheres
  • Preparation method and application of monodisperse magnetic polydopamine microspheres

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preparation example Construction

[0036] The preparation method of the above-mentioned monodisperse magnetic polydopamine microspheres comprises the following steps:

[0037] Step 1, using high temperature solvothermal core Fe 3 O 4 Nanoparticles:

[0038] Dissolve 65 mg of anhydrous ferric chloride, 3.0 g of anhydrous sodium acetate and 1.0 g of PSSMA in a 40 mL conical flask of ethylene glycol solvent, promote the dissolution and stir by ultrasound, then add 150 μL of ultrapure water, and continue to stir for 1 After 1 hour, 0.6 g of sodium hydroxide powder was added, and the mixture was stirred for 1 hour and then transferred to a 50 mL polytetrafluoroethylene autoclave. Put it in an oven preheated to 190-195°C and react for 9-10 hours; after the reaction, it is naturally cooled to room temperature, the product is separated by an external magnet, washed with absolute ethanol and ultrapure water, and the product is collected by freeze-drying.

[0039] The anhydrous ferric chloride, anhydrous sodium acetat...

Embodiment 1

[0052] (1) Preparation of magnetic core Fe 3 O 4 Nanospheres

[0053] Dissolve 65 mg of anhydrous ferric chloride, 3.0 g of anhydrous sodium acetate and 1.0 g of PSSMA in a 40 mL conical flask of ethylene glycol solvent, promote the dissolution and stir by ultrasound, then add 150 μL of ultrapure water, and continue to stir for 1 After 1 hour, add 0.6 g of sodium hydroxide powder, continue to stir for 1 hour and then transfer to a 50 mL polytetrafluoroethylene autoclave, place it in an oven preheated to 190 to 195 ° C, and react for 9 to 10 hours. After the end, it was naturally cooled to room temperature, the product was separated by an external magnet, washed with absolute ethanol and ultrapure water, and the product was collected by freeze-drying.

[0054] (2) Preparation of Monodisperse Magnetic Polydopamine Fe 3 O 4 @PDA Microspheres

[0055] Weigh 100 mg of the magnetic core Fe prepared above 3 O 4 The nanospheres were dispersed into a mixed solution of 15mL of et...

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Abstract

The invention provides a preparation method and application of monodisperse magnetic polydopamine microspheres, and belongs to the technical field of synthesis of magnetic materials. The technical scheme is as follows: the preparation method comprises the following steps: firstly synthesizing a Fe3O4 magnetic core by a solvothermal method, then mixing the magnetic core with a dopamine monomer, mesitylene, a surfactant F127 and ammonia water to react, and synthesizing a monodisperse magnetic polydopamine microsphere with a core-shell structure by an ultrasonic-assisted method; according to the application, the monodisperse magnetic polydopamine microspheres are applied to nucleic acid extraction, protein extraction or protein purification. The preparation method has the beneficial effects that the preparation process is good in stability, high in preparation efficiency, low in production cost and environmentally friendly, and the obtained magnetic microspheres have the advantages of monodispersity, low sedimentation rate, uniform particle size, high magnetic separation speed and mild separation conditions.

Description

technical field [0001] The invention relates to the technical field of magnetic material synthesis, in particular to a preparation method and application of monodisperse magnetic polydopamine microspheres. Background technique [0002] Magnetic composite microspheres, as a new type of nanomaterials, are hybrid magnetic particles with sizes ranging from several hundred nanometers to several micrometers, which are composed of magnetic inorganic nanoparticles and polymers. They are the most widely used in vitro diagnosis. a basic material. On the one hand, on the basis of this material, by introducing functional groups on its surface and coupling with biomolecules such as antibodies, antigens or nucleic acids, high-sensitivity quantitative detection of the target can be achieved; on the other hand, due to this The magnetic composite microspheres can be easily manipulated by an external magnetic field, thus enabling fast, automated, and multi-throughput high-efficiency detectio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02B01J20/26B01J20/28B01J20/30C07K1/14C12N15/10
CPCB01J13/02B01J20/262B01J20/28009B01J20/3085B01J20/3071C07K1/14C12N15/1006
Inventor 汪洋戴鸿君
Owner 南通伯仲纳米科技有限公司