Microelectrode sensor for detecting staphylococcus aureus as well as preparation method and application method of microelectrode sensor

Abstract

The invention provides a microelectrode sensor for detecting staphylococcus aureus as well as a preparation method and an application method of the microelectrode sensor. The microelectrode sensor is a three-electrode system sensor, a counter electrode of the microelectrode sensor is a platinum wire electrode, a reference electrode of the microelectrode sensor is an Ag/AgCl electrode, and a working electrode of the microelectrode sensor is a silver microelectrode modified by chitosan, catechol and a staphylococcus aureus aptamer. The microelectrode sensor disclosed by the invention is constructed on the basis of a microelectrode with a micro-nano structure on the surface, double media (Fc-Ru < 3 + >), chitosan and catechol. The working electrode of the microelectrode sensor can specifically capture staphylococcus aureus. In the CV test process of the microelectrode sensor, CI <-> in bacteria can leak to the surface of the working electrode under the action of local high field intensity, and when CI <-> ions exist, the cathode reduction peak of the working electrode between 0 and-0.1 V can be specifically enhanced, so that a linear relation between bacterial concentration increase and current signal enhancement is established; the purpose of specifically detecting bacteria is achieved.

Description

technical field [0001] The invention relates to a microelectrode sensor for detecting Staphylococcus aureus, a preparation method and an application method thereof, and belongs to the technical field of biosensors. Background technique [0002] Staphylococcus aureus was first discovered and named by Dr. Alexander Ogston in 1880. Typical Staphylococcus aureus is spherical, grape-like clusters, about 0.8 μm in diameter, and Gram staining is positive. Staphylococcus aureus is a common pathogen that commonly colonizes human skin and mucosal surfaces, especially the anterior nares (approximately 30% of the general population). When the host's immunity is weakened or the skin and mucosal barriers are disrupted, it can enter any organ or enter the blood, causing skin and soft tissue infections (impetigo, folliculitis, and scalded skin syndrome) in mild cases and severe systemic diseases such as bacteremia, endocarditis, osteomyelitis, hemolytic pneumonia, and toxic shock syndrome...

AI Technical Summary

Problems solved by technology

The gold standard for the detection of S. aureus is still the culture method, but it is very time consuming, usually taking 1-2 days to obtain a single colony, and then 1-2 days to obtain the chemical identification and susceptibility results

In recent years, some rapid and automated detection methods have been developed, such as enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), flow cytometry, and mass spectrometry, although results can be obtained in only 1-5 hours, However, these methods require expensive instruments, complicated sample preparation, and high professional requirements, and are not suitable for remote and poor areas, nor are they suitable for point-of-care testing

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