Bacillus velezensis and application thereof
A Bacillus and strain technology, applied in the application, bacteria, microorganism and other directions, can solve the problems of affecting the quality of livestock and poultry products, destroying the ecosystem, and harming health, and achieve the effects of human and animal safety, high biomass and rapid growth.
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Embodiment 1
[0038] Extraction and Purification of Bacillus Velez
[0039] Put the earthworms in 75% alcohol for surface disinfection, cut along the surface of the earthworms and spread on the LB medium, culture for 12-24 hours, purify the cultured bacteria for 2-3 generations, and insert the single colony into the LB liquid Culture medium for 12-18 hours.
Embodiment 2
[0041] Gram stain, microscopic examination
[0042] The bacteria cultured for 12 hours were stained with Gram and examined under an optical microscope. The macroscopic morphology of the culture medium was shown in Figure 4 As shown in A, using the imaging system to take pictures as Figure 4 Shown in B; cells cultured for 12 hours were collected and fixed overnight at 4°C with glutaraldehyde. The bacteria were collected by centrifugation, rinsed with phosphate buffer, dehydrated with ethanol gradient, and then dried at the critical point of carbon dioxide. After spraying gold, the morphology of the bacteria was observed with a scanning electron microscope Hitachi SU8010, as shown in Figure 4 C shown.
[0043] Conclusion: The colony of Y1 bacteria on TSA medium is yellow, round, with moist surface, opaque, and neat edges, and forms a thin film in liquid medium; the bacteria are short rod-shaped in microscopic examination and can produce spores.
Embodiment 3
[0045] Physiological and biochemical identification
[0046] According to the "Common Bacteria System Identification Manual" and "Bergey's Bacterial Identification Manual" (9th Edition), the species of the isolated bacteria were initially identified, and the results are shown in Table 1.
[0047] Table 1
[0048]
[0049] Conclusion: Y1 bacteria can utilize glucose, mannitol, raffinose, fructose, maltose, ancient sugar, D-trehalose, D-ribose; can hydrolyze starch and aescin, produce gelatinase and catalase, and can Growth in 6.5% sodium chloride; unable to produce hydrogen sulfide, unable to utilize L-rhamnose, D-melezitose and D-galactose.
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