Method for preparing fruit and vegetable fresh-keeping agent through fermentation and extraction of sophora flower buds
A fruit and vegetable preservative and extraction method technology, applied in the direction of fruit and vegetable preservation, bacteria used in food preparation, food ingredients containing natural extracts, etc. Capacitance, simplify the production process, and ensure the effect of product quality
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[0036] Extraction method of Zanthoxylum bungeanum extract:
[0037] Take 10g of Zanthoxylum bungeanum powder, add 150mL of methanol as a solvent, put it in a 250mL Erlenmeyer flask, ultrasonicate at 25°C and 500W power for 8h, filter through a 0.22μm filter membrane, and rotary evaporate at 65°C for 2h to obtain the Zanthoxylum bungeanum extract.
[0038] Extraction method of lychee grass extract:
[0039] Lychee grass was dried, crushed, passed through a 20-mesh sieve, weighed 10 g, added 100 mL of 70% ethanol solution, placed in a 250 mL Erlenmeyer flask, and subjected to ultrasonic extraction at 30 °C and 500 W for 3 times, and after each ultrasonic treatment for 1.5 h, the Filtrate through a 0.22 μm filter membrane, combine the filtrates, and spin at 60°C to concentrate the volume to 1 / 5 to obtain the lychee grass extract.
Embodiment 1
[0041] A method for preparing a fruit and vegetable preservative by fermenting and extracting Sophora japonica, comprising the steps of:
[0042] (1) Take by weighing 3.0kg of pulverized Chinese pagoda japonica coarse powder, prepare strain fermentation substrate by 8L (wherein, glucose 59g / L, yeast extract 20g / L, potassium dihydrogen phosphate 12g / L, magnesium sulfate heptahydrate 0.6 g / L, manganese sulfate tetrahydrate 0.05g / L, anhydrous calcium carbonate 15g / L, sodium chloride 3g / L), be placed in 50L fermentation tank, add sterile water 30L, stir;
[0043] (2) The fermenter was sterilized at 125°C and 0.08M Pa for 30 minutes, and was inoculated with 3.5L of bacteria at a concentration of 1×10 when cooled to room temperature. 11 For Lactobacillus fermentum seed solution per L, set the pH in the fermenter to 6.5, temperature 32°C, rotation speed 150rpm, ventilation rate 0L / min, and carry out anaerobic fermentation for 48h;
[0044] (3) Add 3.5L strain concentration 1×10 to t...
Embodiment 2
[0048] A method for preparing a fruit and vegetable preservative by fermenting and extracting Sophora japonica, comprising the steps of:
[0049] (1) Take by weighing 2.8kg of pulverized Chinese pagoda japonica coarse powder, prepare strain fermentation substrate by 6L (wherein, glucose 59g / L, yeast extract 20g / L, potassium dihydrogen phosphate 12g / L, magnesium sulfate heptahydrate 0.6 g / L, manganese sulfate tetrahydrate 0.05g / L, anhydrous calcium carbonate 15g / L, sodium chloride 3g / L), be placed in 50L fermentation tank, add sterile water 30L, stir;
[0050] (2) The fermenter was sterilized at 125°C and 0.08M Pa for 30 minutes, and when cooled to room temperature, 3L of bacteria was inoculated with a concentration of 1×10 11 For Lactobacillus fermentum seed solution per L, set the pH in the fermenter to 6.5, temperature 32°C, rotation speed 130rpm, ventilation rate 0L / min, and carry out anaerobic fermentation for 54h;
[0051] (3) Add 3L strain concentration 1×10 to the ferm...
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