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Method for chemiluminiscence detection of hypochlorite

A chemiluminescence detection and hypochlorite technology, which is applied in the field of analytical chemistry detection, can solve the problems of complex molecular design and high synthesis cost, and achieve high sensitivity and selectivity, convenient operation, and rapid qualitative and quantitative detection.

Pending Publication Date: 2022-05-17
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, there have been more and more studies on the detection of hypochlorite with fluorescent small molecule probes, but the recognition of reactive oxygen species mainly depends on specific recognition groups or reaction sites. Most of the molecular designs are complex and the synthesis cost is high.

Method used

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  • Method for chemiluminiscence detection of hypochlorite
  • Method for chemiluminiscence detection of hypochlorite
  • Method for chemiluminiscence detection of hypochlorite

Examples

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Effect test

Embodiment 1

[0025] A 50 mM PBS (pH=7.4) buffer solution was prepared and used to dissolve the fluorescent gold nanocluster powder synthesized with glutathione as a ligand to obtain a 1.0 mg / mL probe stock solution. 200 μL of the probe stock solution was added to the quartz reaction cell, and placed in the sample chamber of the chemiluminescence analyzer, and a microsyringe was used to draw 200 μL of the active oxygen solution. The active oxygen species in the active oxygen solution were hydrogen peroxide, Hypochlorite ion, singlet oxygen, hydroxyl radical, superoxide anion, tert-butanol peroxide, tert-butanol peroxide radical, alkane peroxyl radical and peroxynitrite, the concentration of each active oxygen species is 200 μM was injected into the quartz reaction cell through the injection port, and the corresponding chemiluminescence intensity was measured on the chemiluminescence analyzer. Chemiluminescent test PMT working voltage is -1100V; time interval is 0.1s. Draw the chemiluminesc...

Embodiment 2

[0027] A 50 mM PBS (pH=7.4) buffer solution was prepared and used to dissolve the fluorescent gold nanocluster powder synthesized with glutathione as a ligand to obtain a 1.0 mg / mL probe stock solution. Mix 200 μL of the probe stock solution and 20 μL of other analyte solutions (other analytes are: potassium ions, cadmium ions, zinc ions, barium ions, calcium ions, magnesium ions, chromium ions, iron ions, aluminum ions, bromide ions, Peroxodisulfate ion, bicarbonate ion, carbonate ion, nitrite ion, nitrate ion, acetate ion, sulfate ion, adenosine triphosphate, and other analytes at a final concentration of 100 μM) added to quartz Reaction cell, and placed in the sample compartment of the chemiluminescence analyzer. Use a microsyringe to draw 200 μL of hypochlorite solution with a concentration of 200 μM, inject it into the quartz reaction cell through the injection port, and measure the corresponding chemiluminescence intensity on a chemiluminescence analyzer. Chemiluminesce...

Embodiment 3

[0029] A 50 mM PBS (pH=7.4) buffer solution was prepared and used to dissolve the fluorescent gold nanocluster powder synthesized with glutathione as a ligand to obtain a 1.0 mg / mL probe stock solution. Add 200 μL of the probe stock solution into the quartz reaction cell, and place it in the sample chamber of the chemiluminescence analyzer, draw 200 μL of hypochlorite solutions of different concentrations (20, 50, 80, 150, 250, 350, 450, 550, 650 μM) with a micro-syringe, and inject them respectively through the injection port into the quartz reaction cell, and measure the corresponding chemiluminescence intensity on the chemiluminescence analyzer at the same time. Chemiluminescent test PMT working voltage is -1100V; time interval is 0.1s. Get log value (log C) with concentration as abscissa, take chemiluminescent intensity (I) as ordinate plotting figure, obtain the working curve of hypochlorite concentration; Linear regression equation is: I=94149log C-102502 (the unit of C ...

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Abstract

The invention discloses a method for detecting hypochlorite through chemiluminescence. According to the method, in a PBS buffer solution, a chemical reaction between glutathione ligand on the surface of the gold nanocluster and hypochlorite is utilized to generate an excited-state intermediate, then energy is transferred to the fluorescent gold nanocluster, an optical radiation signal is generated, and detection of hypochlorite is achieved. According to the method, simple and rapid qualitative and quantitative detection of the hypochlorite is realized, and meanwhile, other analytes do not interfere with determination of the hypochlorite. Compared with the existing fluorescence quenching probe based on the reaction of the fluorescent gold nanocluster and active oxygen, the detection method disclosed by the invention does not need an excitation light source, has low background signal and has high sensitivity and selectivity to hypochlorite; detection equipment is simple, convenient to operate, high in response speed, low in cost and accurate in detection result.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry detection, and in particular relates to a method for detecting hypochlorite by chemiluminescence. Background technique [0002] Active oxygen is a general term for a class of oxygen-containing substances that are more active than oxygen molecules in organisms or in the natural environment. A large number of studies have shown that excessive reactive oxygen species can induce a variety of diseases in the body, such as cancer, cardiovascular disease, neurodegenerative disease, rheumatoid arthritis, DNA damage, etc. Among them, hypochlorite is an important reactive oxygen species, which is essential for maintaining normal physiological functions of the body. However, the imbalance of hypochlorite concentration in the body will lead to apoptosis, cause tissue damage, and affect the normal physiological activities of the body. Therefore, it is necessary to develop highly sensitive and se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 周文娟曹加婷
Owner CAPITAL NORMAL UNIVERSITY
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