Total nutrient formula food for special medical purpose for tumor patients and preparation method of total nutrient formula food

A technology for cancer patients and formula food, which is applied in the directions of food ingredients, functions of food ingredients, food ingredients containing oil, etc. It can solve the problems of not being a complete nutritional formula, not fully meeting the nutritional needs of tumor populations, and not belonging to, and improving nutrition. Adverse effects, reduced hospitalization costs, and high nutritional value

Pending Publication Date: 2022-05-27

NANCHANG UNIV

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AI-Extracted Technical Summary

Problems solved by technology

At the same time, the technical problem to be solved is that most of the products suitable for tumor patients on the market are not complete nutritiona...

Method used

Marine collagen peptide is formed by enzymatic hydrolysis of the skin of deep-sea fish-salmon widely distributed in the North Pacific Ocean and the Atlantic Ocean, has multiple biological activities, and is rich in glycine, glutamic acid, proline and hydroxyproline Amino acids, most of which are oligopeptide compounds derived from enzymatic hydrolysis of collagen in marine fish scales, cartilage and bones. Studies have found that fish collagen peptides have high activity in scavenging free radicals and inhibiting lipid peroxidation in vitro. Polypeptide collagen (MCP) was prepared from salmon skin, and American Sprague-Dawley rats were selected as experimental subjects (40 rats in each group, the ratio of male to female was 1:1) for research , add 0%, 2.25%, 4.5% and 9% of MCP (accounting for total energy) respectively, observe the survival period and spontaneous tumor incidence rate of rats when they were 4 weeks old to natural death, the results show that MCP has an important effect on the whole life of rats. Neither body weight nor food intake was significantly affected by cycle. Experiments have demonstrated that MCP dose-dependently inhibits the age-related decrease in antioxidant enzyme activity and the age-related increase in the level of amphoteric lipid peroxidation products. The study found that MCP significantly prolongs the average lifespan and maximum survival period of rats, reduces the total spontaneous tumor incidence in both female and male rats, and the tumor mortality rate in the MCP group is significantly lower than that in the control group. Therefore, we concluded that marine collagen peptide dose-dependently prolongs the lifespan and reduces the incidence of spontaneous tumors in Sprague-Dawley rats.

People such as Midtbo Lisa Kolden have studied the influence of different soybean oil intake levels on tumor mouse carbohydrate metabolism and inflammatory response, and mice are fed different rations, and wherein soybean oil accounts for 5%, 10%, 15% of total energy %, 20%, 25% and 30%, to analyze the gene expression of glycemic index, blood lipid index, inflammatory factors, insulin receptor substrate 1 (IRS1) and insulin receptor substrate 2 (IRS...

Abstract

According to the full-nutrient formula food for the special medical purpose for the tumor patients, a reasonable formula is designed, and the full-nutrient formula food serves as a nutrient supplement way for the tumor patients and plays a basic nutrient support role; the polypeptide component is adopted as a main protein source of the full-nutrition formula food for the special medical purpose of tumors, rapid absorption of tumor patients is facilitated, energy requirements of postoperative patients are supplemented in time, and the full-nutrition formula food is prepared by combining other nutrients and raw materials through a special process; the component structure of high fat, high protein and low carbon water of the formula not only provides comprehensive nutritional requirements for tumor patients, but also is beneficial to enhancing the anti-tumor effect of the patients, correcting negative nitrogen imbalance of the tumor patients, reducing complications and the like; meanwhile, some nutrient supplements are added into the formula, so that the clinical immunity of tumor patients is remarkably improved, various treatment means are enhanced, and rehabilitation is promoted.

Application Domain

Vitamin food ingredientsInorganic compound food ingredients +3

Technology Topic

NutrientNutrition +7

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Examples

Experimental program(3)

Example Embodiment

[0039] Example 1:

[0040] A complete nutritional formula food for special medical purposes for cancer patients, 100 g of the formula food contains: 14 g of hydrolyzed whey protein, 10 g of fish collagen peptide, 16 g of vegetable fat powder, 8 g of medium chain triglycerides, 30 g of maltodextrin, 10 g of sucrose, Soybean dietary fiber 4g, inulin 3g, arginine 2.3g, glutamine 0.6g, taurine 110mg, L-carnitine 20mg, complex minerals 1.2g, multivitamins 110mg, the balance is sodium caseinate.

[0041] The 1.2g compound minerals added are: sodium citrate 400mg, potassium chloride 450mg, magnesium carbonate 50mg, ferrous sulfate 2mg, zinc gluconate 3mg, sodium selenite 20μg, copper sulfate 250μg, manganese sulfate 350μg, Sodium iodide was 40 μg, and the balance was calcium hydrogen phosphate.

[0042] The added amount of the 110mg multivitamin is: vitamin A 450μg, vitamin D 1.9μg, vitamin E 22mg, vitamin B 1 0.5mg, vitamin B 2 0.6mg, vitamin K 1 18μg, vitamin B 6 0.7mg, vitamin B 12 0.8μg, niacin 4mg, folic acid 40μg, pantothenic acid 1.5mg, biotin 10μg, the balance is vitamin C.

[0043] The preparation method of the formula food for special medical purpose for cancer patients includes the following steps:

[0044] (1) The nutrients are mixed by dry method and wet method. Non-heat-sensitive materials are mixed by wet method, and heat-sensitive materials are mixed by dry method.

[0045] (2) Dry mixing: The heat-sensitive materials are mixed with a pull-out double-blade dry mixer, and the heat-sensitive materials (polypeptide components, amino acid components, nutritional fortifiers, multivitamins, and multiminerals) are added to the dry mixer. mix. The forward and reverse rotation speed of the dry mixer is 20r/min, each time is 20min, and the mixing is 2-3 times.

[0046] (3) Wet mixing: Dissolve non-heat-sensitive materials such as carbohydrate components, lipid components, and dietary fiber components in distilled water (temperature: 50°C, ratio: 1:3 (m/v)) for mixing, whipping at low speed (mixer rotation speed 160r/min, 20min), the obtained solution was subjected to high-speed shearing in a high-speed shearing emulsifier (6000r/min, 3min), and then passed through a high-pressure homogenizer (pressure 30MPa-80MPa, flow rate 20-50m/s) Homogenize once, and then enter into a low-temperature spray dryer (air inlet temperature: 150°C, outlet air temperature: 80°C, feeding speed: 8 mL/min) to obtain powdery products.

[0047] (4) Blending: the powdery materials obtained by dry blending and wet blending are blended in a pull-out double-blade dry blender to obtain the final powdery product. The parameters of the dry mixer are the same as in step (2).

[0048] (5) Sterilization: Sterilize the mixed material.

Example Embodiment

[0049] Example 2:

[0050] A complete nutritional formula food for special medical purpose of tumor patients, 100g of formula food contains: 12g hydrolyzed whey protein, 12g fish collagen peptide, 18g soybean oil powder, 6g medium chain triglyceride, 34g maltodextrin, oligofructose 4g, galactooligosaccharide 2g, soybean dietary fiber 5g, polydextrose 2g, arginine 2.6g, glutamine 0.7g, taurine 100mg, L-carnitine 30mg, complex minerals 1.3g, multivitamin 64mg, more The amount is sodium caseinate.

[0051] The added amount of the 1.3g complex minerals is: sodium citrate 350mg, potassium chloride 580mg, magnesium carbonate 90mg, ferrous sulfate 6mg, zinc gluconate 7mg, sodium selenite 30μg, copper sulfate 300μg, manganese sulfate 350μg, Sodium iodide was 40 μg, and the balance was calcium hydrogen phosphate.

[0052] The added amount of the 64 mg multivitamin is: vitamin A 600 μg, vitamin D 2.5 μg, vitamin E 10 mg, vitamin B 1 0.9mg, vitamin B 2 1.2mg, vitamin K 1 25μg, vitamin B 6 0.7mg, vitamin B 12 0.8μg, niacin 4mg, folic acid 40μg, pantothenic acid 1.5mg, biotin 10μg, the balance is vitamin C.

[0053] The preparation method of the formula food for special medical purpose for cancer patients includes the following steps:

[0054] (1) The nutrients are mixed by dry method and wet method. Non-heat-sensitive materials are mixed by wet method, and heat-sensitive materials are mixed by dry method.

[0055] (2) Dry mixing: The heat-sensitive materials are mixed with a pull-out double-blade dry mixer, and the heat-sensitive materials (polypeptide components, amino acid components, nutritional fortifiers, multivitamins, and multiminerals) are added to the dry mixer. mix. The forward and reverse rotation speed of the dry mixer is 20r/min, each time is 20min, and the mixing is 2-3 times.

[0056] (3) Wet mixing: Dissolve non-heat-sensitive materials such as carbohydrate components, lipid components, and dietary fiber components in distilled water (temperature: 60°C, ratio: 1:3.5 (m/v)) for mixing, whipping at low speed (mixer rotation speed 180r/min, 25min), the obtained solution was subjected to high-speed shearing in a high-speed shearing emulsifier (7000r/min, 4min), and was homogenized by a high-pressure homogenizer (pressure 30MPa-80MPa, flow rate 20-50m/s) quality once, and then enter into a low-temperature spray dryer (inlet air temperature: 180°C, outlet air temperature: 90°C, feed rate: 9mL/min) to obtain powdery products.

[0057] (4) Blending: the powdery materials obtained by dry blending and wet blending are blended in a pull-out double-blade dry blender to obtain the final powdery product. The parameters of the dry mixer are the same as in step (2).

[0058] (5) Sterilization: Sterilize the mixed material.

Example Embodiment

[0059] Example 3:

[0060]A complete nutritional formula food for special medical purposes for tumor patients, 100 g of formula food contains: 10 g of hydrolyzed whey protein, 14 g of fish collagen peptide, 13 g of non-dairy creamer, 11 g of soybean oil powder, 25 g of maltodextrin, 10 g of sucrose, oligomeric Fructose 3.5g, Galactooligosaccharide 1.5g, Soybean Dietary Fiber 3g, Polydextrose 2g, Resistant Dextrin 2g, Arginine 1.6g, Glutamine 0.5g, Taurine 130mg, L-Carnitine 45mg, Complex Mineral The substance is 1.34g, the multivitamin is 85mg, and the balance is sodium caseinate.

[0061] The 1.34g compound minerals added are: sodium citrate 300mg, potassium chloride 600mg, magnesium carbonate 105mg, ferrous sulfate 8mg, zinc gluconate 10mg, sodium selenite 20μg, copper sulfate 260μg, manganese sulfate 170μg, Sodium iodide is 35 μg, and the balance is calcium hydrogen phosphate.

[0062] The 85mg multivitamin addition amount is: vitamin A 700μgRE, vitamin D 5μg, vitamin E 16mgα-TE, vitamin B 1 0.9mg, vitamin B 2 1.2mg, vitamin K 1 25μg, vitamin B 6 0.7mg, vitamin B 12 0.8μg, niacin 4mg, folic acid 40μg, pantothenic acid 1.5mg, biotin 10μg, the balance is vitamin C.

[0063] The preparation method of the formula food for special medical purpose for cancer patients includes the following steps:

[0064] (1) The nutrients are mixed by dry method and wet method. Non-heat-sensitive materials are mixed by wet method, and heat-sensitive materials are mixed by dry method.

[0065] (2) Dry mixing: The heat-sensitive materials are mixed with a pull-out double-blade dry mixer, and the heat-sensitive materials (polypeptide components, amino acid components, nutritional fortifiers, multivitamins, and multiminerals) are added to the dry mixer. mix. The forward and reverse rotation speed of the dry mixer is 20r/min, each 20min, and the mixing is 2-3 times.

[0066] (3) Wet mixing: Dissolve non-heat-sensitive materials such as carbohydrate components, lipid components, and dietary fiber components in distilled water (temperature: 65°C, ratio: 1:4 (m/v)) for mixing, whipping at low speed (mixer rotation speed 200r/min, 30min), the obtained solution was subjected to high-speed shearing in a high-speed shear emulsifier (8000r/min, 5min), and was homogenized by a high-pressure homogenizer (pressure 30MPa-80MPa, flow rate 20-50m/s) quality once, and then enter into a low-temperature spray dryer (air inlet temperature: 190 °C, air outlet temperature: 95 °C, feeding speed: 10 mL/min) to obtain powdery products.

[0067] (4) Blending: the powdery materials obtained by dry blending and wet blending are blended in a pull-out double-blade dry blender to obtain the final powdery product. The parameters of the dry mixer are the same as in step (2).

[0068] (5) Sterilization: Sterilize the mixed material.

[0069] 1. Document support of the complete nutritional formula food for special medical purposes of tumor of the present invention

[0070] 1) Protein: There are many sources of protein in the examples of the present invention, and the present invention takes marine collagen peptide (MCP) and whey protein as examples for literature description.

[0071] (1) Fish collagen

[0072] Marine collagen peptides are enzymatically hydrolyzed from the skin of salmon, a deep-sea fish widely distributed in the North Pacific and the Atlantic Ocean, and have a variety of biological activities, rich in glycine, glutamic acid, proline and hydroxyproline. Most of these are oligopeptide compounds derived from collagenase hydrolysis in fish scales, cartilage and bones. Studies have found that fish collagen peptides have high activity in scavenging free radicals and inhibiting lipid peroxidation in vitro. Polypeptide collagen (MCP) was prepared from salmon skin as raw material, and American Sprague-Dawley rats were selected as experimental subjects (40 rats in each group, with a male-to-female ratio of 1:1) for research. , adding 0%, 2.25%, 4.5% and 9% of MCP (total energy), respectively, to observe the survival period and spontaneous tumor incidence of rats from 4 weeks of age to natural death. Cycle weight and food intake were not significantly affected. The experiments demonstrated that MCP dose-dependently inhibited the age-related decrease in antioxidant enzyme activity and the age-related increase in the level of amphoteric lipid peroxidation products. The study found that MCP significantly prolonged the average lifespan and maximum survival time of rats, decreased the total spontaneous tumor incidence in female and male rats, and significantly reduced tumor mortality in the MCP group compared with the control group. Therefore, we conclude that marine collagen peptides dose-dependently prolong lifespan and reduce spontaneous tumor incidence in Sprague-Dawley rats.

[0073] (2) Whey protein

[0074] Whey protein is rich in arginine and branched-chain amino acids. Studies have found that supplementing glutamic acid, arginine and branched-chain amino acids can help improve clinical symptoms and reduce complications in cancer patients. At the same time, the clinical controlled trial of Berk L et al. found that supplementing amino acids such as glutamate, arginine and leucine to lean tumor patients with stage 3, 4 or distant metastasis did not increase lean body tissue and could improve the clinical symptoms of tumor patients. Bounous G et al reported that whey protein is easy to digest and has biological activity to inhibit tumor growth. Attaallah W et al. fed whey protein whole protein and whey protein hydrolyzate to animals respectively, and observed the tumor formation of carcinogens, and found that the tumor formation rate of whey protein hydrolyzate-fed animals decreased significantly. There are many similar studies, but All are limited to animals and need further confirmation by clinical trials.

[0075] Liberman Keliane et al. investigated the protective effects of two common dietary proteins, bovine whey albumin and soy protein isolate, on mammary tumors in female SD rats. Rats used casein, soy protein or whey protein as the only source of protein. When the rats in the 100% casein-fed group began to develop tumors, the rats in each group grew well, but the weight of the casein-fed rats was slightly lower. higher than soy protein or whey protein-fed rats (p<0.05); when 50% casein-fed rats developed tumors, the soy protein-fed group (p<0.009) and the whey protein-fed group (p<0.001) The tumor incidence was low (24% to 34%). When 100% casein-fed rats developed tumors, soy protein-fed rats had a higher incidence of tumors (77%) in Experiment B (p < 0.002), but there was no significant difference in Experiment A (p > 0.002). 0.05). The incidence (54%-62%, p<0.002) and diversity (p<0.007) of mammary gland tumors in whey-fed rats were lower than those in casein-fed rats. The experimental results showed that a diet rich in soy protein reduced the incidence of chemically induced breast tumors by about 20%, and that whey protein was at least twice as effective as soy protein in reducing tumor incidence and tumor diversity. Kennedy et al studied the effect of 30 g of whey protein daily over a 6-month period in 7 patients with metastatic cancer who received chemotherapy: In two patients, tumor size was reduced by 28%, while in the other two patients, tumor size was reduced by 28%. Size stabilized, and the remaining three patients showed no change in tumor progression. The researchers confirmed that two patients had a persistent decline in lymphocyte GSH levels to normal levels.

[0076] 2) Fat: There are many sources of fat in the examples of the present invention, and the present invention takes soybean oil and medium-chain triglycerides as examples for literature description.

[0077] (1) Soybean oil

[0078] Kagawa et al. investigated the effect of slow intravenous infusion of a soybean oil-based fat emulsion on immune parameters in patients with esophageal cancer resection. Thirty-two patients after resection of esophagus cancer were randomly divided into a fat emulsion treatment group (LPD, 20% soybean oil emulsion (total energy)) and a control group (receiving and treatment group defat PN with equal energy) for 7 consecutive days. All patients received parenteral feeding (glucose-based solution). The proliferation of lymphocytes and the concentration of serum monocyte chemoattractant protein-1, interleukin-6 and tumor necrosis factor-α were detected. The results of the study found that there was no significant difference in the concentrations of plasma monocyte chemoattractant protein-1, interleukin-6 and tumor necrosis factor-α between the two groups (p>0.05). Compared with the control group, the lymphocyte proliferation response in the LPD group was significantly reduced after esophageal cancer resection, proving that the production of immune cells in the body's immune system was reduced in esophageal cancer patients after receiving soybean oil diet after surgery. Thus, soybean oil-fat emulsions can slow tumor cell growth in esophageal cancer patients when administered slowly.

[0079] Midtbo Lisa Kolden et al. studied the effect of different soybean oil intake levels on carbohydrate metabolism and inflammatory response in tumor mice. The mice were fed different diets in which soybean oil accounted for 5%, 10%, 15%, and 20% of total energy. %, 25% and 30%, the gene expression of glycemic index, blood lipid index, inflammatory factors, insulin receptor substrate 1 (IRS1) and insulin receptor substrate 2 (IRS2) were analyzed. The results showed that high intake of soybean oil can lead to excessive weight gain, increased fasting blood glucose and serum insulin levels, increased levels of tumor necrosis factor-α, interleukin, and high-sensitivity C-reactive protein, and decreased IRS1 and IRS2 mRNA expressions. But in general, it has a certain positive effect on mice, and can improve the symptoms of underweight tumor mice. Thus, dietary supplementation of soybean oil improved tumor-related symptoms in mice.

[0080] (2) Medium chain triglycerides

[0081] Due to its small molecular weight and high water solubility, MCT does not require a carrier or additional energy to enter the mitochondrial oxidation process, so it is superior to LCT in serum clearance and oxidation rate. Reports of adverse effects of LCT mainly focus on the case of LCT infusion doses greater than 2.6g/(kg·d), which far exceeds the general recommended dose. In addition, the domestic PN guidelines recommend that for patients with hyperlipidemia (triglyceride >3.5 mmo/L) and abnormal lipid metabolism, the use of lipid emulsions should be determined according to the patient's metabolic status; while in patients with severe hyperlipidemia Fat emulsions should be avoided in patients with hyperlipidemia (triglycerides > 4-5 mmol/L). Clinical studies have found that a ketogenic diet formulated with MCT can improve epilepsy treatment; at the same time, MCT can inhibit tumor glucose metabolism and growth in tumor patients in a state of ketosis, while maintaining the patient's nutritional status.

[0082] The study found that the fat sources in parenteral nutrition (TPN) solutions were structured lipids composed of regular long-chain triglycerides (soybean oil) and medium-chain triglycerides (MCT), respectively. The TPN solution containing fish oil can clearly see that the tumor growth rate is reduced, and the supplementation of fish oil is by reducing prostaglandin E 2 and its metabolites at the tissue level to play an anti-tumor effect; by changing arachidonic acid metabolism and prostaglandin synthesis, omega-3 fatty acids can change the decomposition rate of tumor proteins and inhibit the proliferation potential of tumors.

[0083] 3) Carbohydrates: In the examples of the present invention, there are many sources of carbohydrates, and the present invention takes maltodextrin as an example for literature description.

[0084] So Eui Young et al. investigated the inhibitory effect of resistant maltodextrin sol (Fiberso1-2) on tumor growth by soft agar colony formation assay. 3% resistant maltodextrin (accounting for the total mass) was added to the experimental group, and no resistant maltodextrin was added to the control group. HCT116 and HCT116 p53(-) cells were inoculated in soft agar to observe the formation of tumor cell colonies. It was found that both HCT116 and HCT116 p53(-) cells in the control group formed colonies within 7-10 days, and the colony diameter of HCT116p53(-) cells was significantly larger than that of HCT116 cells. After adding Fibersol-2 to the experimental group, it was found that both cells could not grow in soft agar, which indicated that Fibersol-2 could directly inhibit the growth of tumor cells.

[0085] Excessive intake of high-GI foods will increase the risk of cancer. The choice of carbohydrates generally takes into account the glycemic index (GI) of the food. Low-GI foods are beneficial to studies. It has also been found that polysaccharides and their polymers play a role in immune regulation, anti-tumor, and anti-inflammatory. It has good pharmacological activities in many aspects, such as anti-radiation, lowering cholesterol, lowering blood pressure and antithrombosis. At the same time, low carbohydrates are beneficial to slow down the growth of tumor cells and have a positive effect on tumor patients. The study found that long-term preoperative fasting in tumor patients undergoing surgery may lead to a catabolic abnormality, accompanied by temporary insulin resistance, inflammation and postoperative discomfort, and maltodextrin can reduce these negative effects, thereby speeding up surgery. recovery after.

[0086] 4) Dietary fiber: There are many sources of dietary fiber in the embodiments of the present invention, and the present invention takes soybean dietary fiber as an example for literature description.

[0087] Soybean dietary fiber has the characteristics of rich nutrition, unique flavor and safe consumption. Studies have found that dietary fiber can reduce the incidence of tumors and reduce the occurrence of complications. At the same time, due to the unique biological properties of dietary fiber, it has a good protective effect on preventing the occurrence and development of tumors. It not only enhances intestinal peristalsis, thereby diluting the concentration of toxins and carcinogens and shortening their residence time in the intestine, but also promotes the growth of probiotics and inhibits the growth of pathogens, thereby inhibiting the growth of carcinogens. The Chinese Nutrition Society recommends that 50% to 60% of the total dietary energy of residents should come from complex carbohydrates (rice, wheat, corn, potatoes, beans, etc.), and the daily intake of dietary fiber per person should be 25 to 35g. It is not only beneficial for the prevention of tumors such as colon cancer and breast cancer, but also for the prevention of hypertension, diabetes and other cardiovascular diseases.

[0088] Some dietary fibers have been shown to reduce the incidence of colon tumors in rats fed a high-fat diet. While insoluble fiber is often associated with this anticancer potential, some soluble fibers, such as fungus, flaxseed, and soybean, have also been shown to inhibit chemically induced colon cancer in rodents. A study has evaluated the effects of wheat bran, flaxseed and soybean alone or in combination on azomethane-induced colon tumor formation in rats fed a high-fat diet. The colons of the rats were used to evaluate the formation of abnormal colorectal crypts (ACF). The results show that different combinations of insoluble fiber (wheat bran) and soluble fiber (soybean, flax) can significantly reduce the number and size of ACF. Compared with bran and flax, the soybean diet significantly reduced the amount of ACF formed (p<0.05). The incidence and multiplicity of ACF were significantly decreased in the flax and wheat bran fiber and soybean compound feed groups (p<0.05).

[0089] 2. The animal test of the complete nutritional formula food for special medical purposes of tumor of the present invention

[0090] 1) Research object

[0091] SPF grade C57BL/6 mice (6-week-old, female, 70 mice) were used as experimental objects, and the cell line was mouse lung cancer cells: Lewis cells.

[0092] 2) Construction of tumor mouse model

[0093] After the Lewis lung cancer cells were recovered and subcultured by the second generation cells, the cells in the logarithmic growth phase were collected and counted after centrifugation. The mice were randomly divided into 7 groups with 10 mice in each group. One group was the normal group without tumor cells, and 6 groups were subcutaneously injected on the right side of the back of each mouse containing 2x10 7 100 μL of cell suspension per mL. On the 11th day after injection of cells, the markers were numbered and weighed.

[0094] 3) Experimental steps

[0095] (1) Feeding method

[0096] The 7 groups were the tumor special medical food experimental groups 1-3, the commercially available Suyusu group, the treatment control group, the model group, and the normal group.

[0097] Tumor special medical food experimental group 1-3: gavage of tumor special medical food + intraperitoneal injection of cisplatin solution;

[0098] Commercially available Suyusu group: gavage of Suyusu + intraperitoneal injection of cisplatin solution;

[0099] Treatment control group: gavage with distilled water + intraperitoneal injection of cisplatin solution;

[0100] Model group: gavage with distilled water + intraperitoneal injection of normal saline;

[0101] Normal group: gavage with equal volume of distilled water + intraperitoneal injection of equal volume of normal saline (no modeling).

[0102] For the above 7 groups, the concentration and equivalent dose were calculated according to body weight. The 7 groups of experiments were given 2 times a day by intragastric administration, and the intragastric dose was 20 μL/g.BW for 10 consecutive days. Except for the model group and the normal group, each group was given cisplatin solution by intraperitoneal injection, 3 times within 10 days, and once on the 1st, 5th, and 8th days after the start of gavage.

[0103] (2) Detection indicators

[0104] a. General observation and body weight

[0105] During the experiment, the mice's mental state, diet, hair, autonomous activity, muscle strength, feces and their response to external stimuli were regularly observed. Each group was weighed once a day before and after gavage on the first day, and the body weight was recorded.

[0106] b. Tumor size

[0107] Before administration and on the 4th, 8th and 11th days after administration, use digital vernier calipers to measure the length and width of the tumor, and calculate the size of the tumor by the formula (tumor volume = length x width 2 /2).

[0108] c. Serum biochemical indicators

[0109] On the last day of the experiment, the eye canthal venous blood of the mice was collected and placed at 4°C for about 6 h. After coagulation, the blood was centrifuged at 5000 r/min for 10 min, and the supernatant was collected. ) content. ELISA kit was used to detect the content of prealbumin (PA) in mouse serum.

[0110] 4) Experimental results

[0111] a. Compared with the treatment control group, the mice in the tumor special medical food experimental group were in good mental state and did not lose weight. (14.5g), the weight loss was relatively small, with a significant difference.

[0112] b. With the model group (tumor size 257mm 3 ) compared with the tumors of mice in experimental groups 1-3 of tumor special medical food (tumor sizes were 137 mm, respectively). 3 , 125mm 3 and 148mm 3 ) growth was significantly inhibited and the volume was significantly reduced; compared with the treatment group (tumor size 75 mm 3 ), the mean tumor volume of the experimental group of the tumor special medical food was relatively larger, with a significant statistical difference (P<0.05).

[0113] c. Compared with the treatment group (TP: 50g/L), the total protein (TP) in the serum of the mice in the tumor special medical food experimental groups 1-3 (TP: 58g/L, 57g/L, 56g/L) Compared with the treatment group (Alb: 23g/L), the experimental group 1-3 (Alb: 24g/L, 25g/L, 25g/L respectively) L) The content of albumin (Alb) in the serum of mice was higher, and the difference was statistically significant (P<0.05); : 285mg/L, 280mg/L, 287mg/L) mice serum prealbumin (PA) content was low, the difference was statistically significant (P<0.05).

PUM

Property	Measurement	Unit
Molecular weight	<= 2.0

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Classification and recommendation of technical efficacy words

high nutritional value
Easy to digest and absorb

Total nutrient formula food for special medical purpose for tumor patients and preparation method of total nutrient formula food

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