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Oral care composition as well as preparation method and application thereof

An oral care and composition technology, applied in the field of biomaterials and oral care, can solve the problems of unsustainable drug release, short residence time, and low specificity, so as to reduce toxic and side effects, reduce the occurrence of bacterial drug resistance, The effect of preventing tooth hard tissue defect

Active Publication Date: 2022-05-27
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the shortcomings of existing oral care products such as short residence time in the oral cavity, unsustainable drug release, and low specificity, the present invention provides a product that can adhere to the surface of teeth, release drugs for a long time, and can quickly release drugs in the acidic environment caused by dental caries. Released oral care composition and method for its preparation and use

Method used

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  • Oral care composition as well as preparation method and application thereof
  • Oral care composition as well as preparation method and application thereof
  • Oral care composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation of micellar composites loaded with tannic acid and sodium fluoride and linked to sialoprotein polypeptides

[0038] (1) Preparation of water-soluble polymer:

[0039] Polyethylene glycol diamine (CAS No.: 24991-53-5, NH 2 C 2 H 2 -(CH 2 O) n -C 2 H 2 NH 2 ) co-reacted with 3-maleimidopropionic acid (MAL-), and MAL was modified on the polymer to obtain the product MAL-PEG-NH 2 .

[0040] ε-(benzyloxycarbonyl)-L-lysine N-carboxyanhydride (ε-benzyloxycarbonyl-L-lysine-N-carboxy-cyclic anhydride, Lys(Z)-NCA) (1.96 g, 6.4 mmol) was dissolved in 30mL of N,N-dimethylformamide (DMF), by adding MAL-PEG-NH 2 (2.0 g, 0.4 mmol), and stirred for 72 h under dry argon at 35 °C to allow polymerization to occur. The solvent was then rotary evaporated and the product was dissolved in 25 mL of CHCl 3 , placed in excess ether for precipitation to obtain the product MAL-PEG-b-PZLL.

[0041] In order to remove the benzyl protecting group, remove the protecti...

Embodiment 2

[0052] Example 2: Drug pH Controlled Release Test

[0053] The drug release from CLM@NaF-Pep under different pH conditions was detected by high performance liquid chromatography and ion exchange chromatography. 2 mL of the freshly prepared CLM@NaF-Pep dispersion was transferred into a dialysis bag (MWCO 3500), then soaked in 15 mL of 10 mM PBS with different pH, and shaken at 37 °C (rotation speed 100 r / min). At fixed time intervals (every 0.5h, 1h, 2h, 4h, 8h, 12h, 24h), 1 mL of dialysate was collected until 24h, and an equal volume of fresh buffer was added every time the dialysate was collected to keep the total volume of dialysate. constant. The contents of tannic acid and sodium fluoride in the dialysate were detected by high performance liquid chromatography and ion exchange chromatography, respectively. Three parallel control groups were set up in all experiments, the average value was calculated and compared, and the experiment was repeated three times.

[0054] The...

Embodiment 3

[0055] Example 3: Test for the effect of inhibiting the growth of Streptococcus mutans

[0056] The Streptococcus mutans cultured to the exponential phase was collected by centrifugation for 3 min (5000 rpm), and diluted to 10 using BHI medium. 6 CFU / mL to obtain bacterial suspension.

[0057] CLM@NaF-Pep was added to BHI medium with pH of 7.4, 6.5 and 5.0, respectively, to prepare the experimental group dispersion; PBS was used as the blank control solution, and chlorhexidine (CHX) was used as the positive control solution. Aliquot into 96-well plates (160 μL per well). Then, 40 μL of the above bacterial suspension was added to the dispersion liquid of the experimental group and the solution of the control group and mixed well. The 96-well plate was placed in a 37°C incubator, and incubated for 0.5, 1, 2, 4, 8, 12, and 24 hours, respectively, and then 100 μL of culture medium was taken from each group and inoculated into a new 96-well plate. The antibacterial activity was ...

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Abstract

The invention discloses an oral care composition and a preparation method and application thereof, the oral care composition comprises a drug-loaded micelle, the drug-loaded micelle comprises a polymer micelle and an enamel repair drug physically coated in the polymer micelle, the polymer micelle is a micelle particle formed by a water-soluble polymer with a structure shown as a formula I in an aqueous solution with tannic acid, and the enamel repair drug is a water-soluble polymer with a structure shown as a formula II. The shell is connected with the sialoprotein polypeptide through a chemical bond. The oral care composition provided by the invention is adhered to the surface of teeth through the sialoprotein polypeptide, a boric acid ester bond between the tannic acid and the water-soluble polymer is opened in an oral acid environment caused by decayed teeth, the tannic acid and sodium fluoride are released, intelligent and on-demand release of drugs can be realized, and the oral care effect is improved. Therefore, the purposes of preventing decayed teeth and repairing tooth defects are achieved.

Description

technical field [0001] The present invention relates to the technical field of biological materials and oral care, in particular to an oral care composition and a preparation method and application thereof. Background technique [0002] Dental caries is one of the most common and high incidence of oral diseases at present, and the resulting tooth defects can lead to the loss of tooth morphological integrity, thus affecting the occlusal function. When multiple teeth are missing in the whole mouth, the occlusal relationship is even lost, and the patient's oral function and aesthetics are seriously damaged. At present, the main treatment method is to reconstruct and repair the defective tissue with repair materials after mechanical and / or chemical removal of the infected tissue, so as to restore the shape and function of the tooth. However, from the perspective of the whole process of tooth defect disease development, this is a remedy after damage is formed, and there may be a...

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K33/16A61K31/7024A61K47/64A61K47/34A61P1/02A61P31/02A61P31/04
CPCA61K9/1075A61K33/16A61K31/7024A61K47/64A61K47/34A61P1/02A61P31/02A61P31/04A61K2300/00
Inventor 黄翠许玥郭景梅
Owner WUHAN UNIV
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