Pharmaceutical composition for improving insulin resistance symptoms
A technology of insulin resistance and composition, which is applied in the directions of drug combination, pharmaceutical formula, and medical preparations containing active ingredients, etc., can solve the problems of unclarified medicinal parts and astragalus polysaccharides.
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Embodiment 1
[0060] Example 1: Determination of Astragalus Polysaccharide Molecular Weight
[0061] Weigh 50.0 mg of astragalus polysaccharide sample into a 10 mL volumetric flask, dissolve with 0.1 mol / L sodium nitrate, and dilute to volume. The samples were separated and analyzed using a Waters 1525 high performance liquid chromatograph with an Ultrahydrogel™ Linear chromatographic column, and the analysis time was 30 min. The standards used in the molecular weight calibration curve in the experiment are as follows: Dextran T-300 (Mw300600), Dextran T-150 (Mw 135030), Dextran T-10 (Mw 9750) and Dextran T-5 (Mw 2700). Test results such as figure 1 As shown, there are 4 peaks in the astragalus polysaccharide sample, the retention time (RT) from small to large are Mw 855109 Da (RT = 15.100), Mw 12503 Da (RT = 18.567), Mw 1388 Da (RT 19.220) , Mw 310 Da (RT= 20.470), according to the detection conditions and research literature, it is speculated that the astragalus polysaccharide samples ...
Embodiment 2
[0062] Example 2: Composition of refined astragalus polysaccharide monosaccharide
[0063] Monosaccharide mixed standard preparation: Weigh glucose, mannose, galactose, galactosamine, ribose, arabinose, rhamnose, fucose, glucuronic acid, galacturonic acid, xylose and glucosamine standard Dissolve 72.0 mg of each in a 10 mL volumetric flask, dissolve in ultrapure water, and dilute to volume to prepare a mother solution with a concentration of 7.2 mg / mL for each standard. Take each mother liquor to prepare a mixed monosaccharide standard solution with each monosaccharide concentration of 0.36 mg / mL.
[0064] Derivation of monosaccharide mixed standard solution: Take 100 μL of mixed monosaccharide standard solution and 100 μL of 0.6 mol / L NaOH solution, respectively, and place them in a 1 mL stoppered test tube and mix well. Then take 50 μL of the mixture into a 5 mL stoppered test tube, add 50 μL of 0.5 mol / L 1-phenyl-3-methyl-5-pyrazolone methanol solution, and mix by vortexin...
Embodiment 3
[0068] Example 3: Establishment of a mouse model of insulin resistance
[0069] 40 male C57BL / 6J mice, SPF grade, 6 weeks old (about 20 g, experimental animal license number: SCXK (Beijing) 2019-0009) were purchased from Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd. Breeding conditions: SPF cleanliness, room temperature 20-22ºC, humidity 60 ± 5%, alternating light and dark for 12 hours, free food and water. All animal protocols in this experiment were approved by the Institutional Laboratory Animal Management and Use Committee of Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd., and animal experiments were performed under the guidance of the Laboratory Animal Protection Association.
[0070] Mice were weighed after 1 week of adaptive feeding, and the mice were randomly divided into normal chow diet (NCD) and high-fat diet (HFD) groups according to their body weight. The NCD group (n = 8) was fed with a normal diet, and the HFD group (n = 32) was fed...
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