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Sponge-sourced biocontrol streptomyces ITBB-ZK-a5 and application thereof

A bio-control streptomyces, itbb-zk-a5 technology, applied in the field of microorganisms, can solve problems such as the impact of pesticide residues on human health, enhanced drug resistance of bacteria, and ecological environment pollution, achieving stable control effects, wide antibacterial spectrum, Environmentally friendly effect

Active Publication Date: 2022-08-05
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Diseases caused by fungi and bacteria are the most important diseases in agricultural production and aquaculture. Chemical pesticides are the most commonly used control methods. However, the widespread use of chemical pesticides will not only enhance the resistance of bacteria, but also cause pollution to the ecological environment. , and more importantly, pesticide residues also have a certain impact on human health

Method used

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  • Sponge-sourced biocontrol streptomyces ITBB-ZK-a5 and application thereof
  • Sponge-sourced biocontrol streptomyces ITBB-ZK-a5 and application thereof
  • Sponge-sourced biocontrol streptomyces ITBB-ZK-a5 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Strain isolation

[0021] The sponge samples collected from the Xisha Islands in the South China Sea were selected as the separation plate of Gao's Synthetic No. 1 medium, and were separated by the dilution coating method. After 7 days of constant temperature incubation at 28°C, the actinomycete strains ITBB-ZK-a5 were observed and picked. Cultured and purified on Streptomyces medium No. 2 (ISP2) medium and stored in a glycerol tube at -20°C.

[0022] Gao's Synthesis No. 1 medium is prepared with the following components in the following mass-volume ratios: soluble starch 20.0g / L, sodium chloride 6.5g / L, ferrous sulfate 0.01g / L, potassium sulfate 1.0g / L, dihydrogen sulfate 20.0g / L Potassium 0.5g / L, magnesium sulfate 0.5g / L, sea salt 17.5g / L, potassium dichromate 50μg / mL, pH 7.3±0.2, add agar 20.0g / L when preparing solid plate (take 1L water as the benchmark, the rest same medium).

[0023] The ISP2 medium is prepared with the following components in the foll...

Embodiment 2

[0025] Example 2 Molecular identification of strain ITBB-ZK-a5

[0026] The actinomycete ITBB-ZK-a5 obtained in Example 1 was inoculated into ISP2 solid medium, cultured at 28°C for 5 days, and a single colony was picked and placed on a fresh ISP2 medium plate for secondary purification and culture for 5 days. The 16S rDNA sequence was determined by Beijing Qingke Biotechnology Co., Ltd. Kunming Branch.

[0027] The 16S rDNA gene sequence of strain ITBB-ZK-a5 (as shown in SEQ ID NO: 1 in the sequence listing) is:

[0028]aaaggaggtgatccagccgcaccttccggtacggctaccttgttacgacttcgtcccaatcgccagtcccaccttcgacggctccctccacaagggttgggccaccggcttcgggtgttaccgactttcgtgacgtgacgggcggtgtgtacaaggcccgggaacgtattcaccgcagcaatgctgatctgcgattactagcgactccgacttcatggggtcgagttgcagaccccaatccgaactgagaccggctttttgagattcgctccacctcacggcttcgcagctcattgtaccggccattgtagcacgtgtgcagcccaagacataaggggcatgatgacttgacgtcgtccccaccttcctccgagttgaccccggcagtctcctgtgagtccccggcataacccgctggcaacacaggacaagggttgcgctcgttgcgggacttaacccaacat...

Embodiment 3

[0030] Example 3 Test of bacteriostatic efficacy of strain ITBB-ZK-a5 against pathogenic fungi

[0031] The pathogenic fungi tested include Fusarium oxysporum f.sp.cubense (Foc), Fusarium oxysporum f.sp.cubense tropical race 4 (Foc4), Colletotrichum musae , Papaya anthracnose fungus (Colletotrichumgloeosporioides Penz.), papaya brown pedicle rot fungus (Phomopsis caricae-papayaeFetrak & Cif.), papaya cocoa chamomile bisporus (Lasiodiplodia theobromae) (the main pathogen causing papaya canker), mango anthracnose fungus (Colletotrichum gloeosporioides), Colletotrichumcapsici, Corynespora cassiicola, Corynespora cassiicola (Berk.et Curt.) Wei, Pythium aphanidermatum (Eds.) Fitzp. , Pestalotiopsis microspora, Thielaviopsis paradoxa (the main pathogen causing coconut fruit rot), Pyricularia oryzae, Fusarium graminearumschw .), Verticillium dahliae, Colletotrichum acutatum, Fusarium solani, Gilbertella persicaria, Peronophythora litchii, Colletotrichum australianum.

[0032] The ...

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Abstract

The invention discloses a strain of biocontrol streptomyces sp. ITBB-ZK-a5 from sponge and a biocontrol product which comprises the biocontrol streptomyces sp. ITBB-ZK-a5 and is used for preventing and treating diseases of crops and aquaculture animals. The biocontrol streptomyces sp. ITBB-ZK-a5 from the sponge and the biocontrol product have a prevention and treatment effect on a plurality of pathogenic bacteria. The invention further discloses application of the biocontrol streptomyces sp. ITBB-ZK-a5 derived from the sponge and a biocontrol product containing the biocontrol streptomyces sp. ITBB-ZK-a5 in the aspect of preparing a medicine with the effect of preventing and treating pathogenic fungi and pathogenic bacteria. The invention further discloses application of the biocontrol product containing the biocontrol streptomyces sp. ITBB-ZK-a5 derived from the sponge and application of the biocontrol product containing the biocontrol streptomyces sp. ITBB-ZK-a5 derived from the sponge in the aspect of preparing the medicine with the effect of preventing and treating the pathogenic fungi and the pathogenic bacteria.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a sponge-derived biocontrol Streptomyces sp. ITBB-ZK-a5 and its application. Background technique [0002] Actinomycetes are a kind of valuable microbial resources. They can produce rich and diverse metabolites and have been widely used in agriculture and health fields. Therefore, people pay more and more attention to mining new potential applications from different environmental conditions. Actinomycetes resources. The ocean's unique environment of high salinity and low nutrition makes marine actinomycetes have physiological structures and functions that are different from terrestrial populations, and form unique metabolic pathways, thus creating abundant marine microbial resources and becoming a huge agricultural Microbial resource library and active metabolite library. The marine sponge belongs to the Porifera of the animal kingdom, which is a large class ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/28A01P1/00A01P3/00A61K35/74A61P31/04C12R1/465
CPCC12N1/205C12N1/20A01N63/28A61K35/74A61P31/04C12R2001/465Y02A40/81
Inventor 郭志凯齐敏熊子君张世清王蓉
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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