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Immune magnetic nano particle bacterium separator and its production method and use

A technology of magnetic nanoparticles and separators, which is applied in the direction of bacteria, biochemical equipment and methods, material inspection products, etc., and can solve the problems of complicated operation process, long time-consuming, high cost, etc.

Inactive Publication Date: 2007-04-25
SHANGHAI NORMAL UNIVERSITY
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  • Description
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Problems solved by technology

[0005] At present, my country's livestock and poultry product safety testing technology mainly adopts some traditional methods such as separation culture and identification, enzyme-linked immunosorbent assay (ELISA) and other methods, which have many defects such as complicated operation process and long detection time. The method used to detect Escherichia coli has the main characteristics. Although it can also accurately detect Escherichia coli O157, it has the disadvantages of taking a long time and high cost. Foreign methods such as immune latex and immune gold detection kits are easy to detect. Condensation, expensive and other issues

Method used

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  • Immune magnetic nano particle bacterium separator and its production method and use
  • Immune magnetic nano particle bacterium separator and its production method and use

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preparation example Construction

[0041] The method for preparing immunomagnetic nanoparticle bacteria separator of the present invention, it comprises the following steps:

[0042] (1) In a microemulsion system containing magnetic nanoparticles and an inner shell forming agent, a core-shell magnetic nanoparticle is formed by a water-in-oil type inverse microemulsion method, and the core-shell magnetic nanoparticle includes a core and shell,

[0043] The magnetic nanoparticles are selected from: iron oxide, iron, nickel, nickel-iron alloy, or a combination thereof;

[0044] The shell forming agent of the inner core layer is selected from the following group: silicon dioxide, agarose, olefin polymer, polyacrylonitrile, epoxy compound, or a combination thereof;

[0045] (2) using [N-(2-aminoethyl)-3-aminopropyltrimethoxysilane] to modify the surface of the core-shell magnetic nanoparticles in step (1) to obtain magnetic nanoparticles modified with amino groups;

[0046](3) Modifying the surface of the amino-mo...

Embodiment 1

[0067] Preparation method of inner core layer of magnetic nanoparticles

[0068] The core layer of magnetic particles was prepared by improved chemical co-precipitation, the specific method is as follows: prepare FeSO 4 .7H 2 O and FeCl 3 .6H 2 O mixed solution and NaOH solution. In a mixed solution of iron salts, Fe 2+ The ion concentration is 0.1~0.2mol / l, Fe 3+ The concentration of ions is 0.1-0.3 mol / l, and the concentration of NaOH solution is 2-3 mol / l. Under vigorous stirring, the NaOH solution whose volume is half of the volume of the mixed salt solution was slowly added dropwise into the mixed salt solution. Aging the obtained solid precipitate at 40°C to 60°C for 12 hours, washing the precipitate several times with double distilled water, filtering and drying at 40°C to 80°C for 24 hours, and grinding it in an agate mortar Get the product.

Embodiment 2

[0070] Preparation method of core-shell nucleic acid magnetic nanoparticles

[0071] Mix TritonX-100, n-hexanol and cyclohexane uniformly at a ratio of 1:2:5 to form a transparent and stable microemulsion system. Put the microemulsion system above into ultrasonic treatment for 30-60 minutes, then add 0.5g of γ-Fe 2 o 3 After 6 minutes of ultrasonic treatment, the supernatant was taken out and poured into a three-necked flask, and stirred for 30 minutes to make it uniform. Take 1ml of ammonia water of a certain concentration and dilute it with 2ml of double distilled water, slowly add it into the microemulsion which is constantly stirred after 30 minutes, and keep stirring for 30 minutes to make the ammonia water evenly dispersed in the microemulsion. After 1 hour, 1-3 ml of tetraethyl orthosilicate was added dropwise to the microemulsion, while stirring continuously for 10 hours, and the temperature of the system was kept between 15-30°C. Add acetone to the system to prec...

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Abstract

The invention relates to a kind of immune magnetism nanometer bacteria disjunctor which has three layers: (1) the inner nuclear layer constructed by nucleocapsid magnetism nanometer particles; (2) the middle layer that capsizes the inner layer (3) the outside antibody / ligand layer that capsizes the middle layer. The way to make disjunctor is also disclosed in the invention. The disjunctor in the invention can separate the target bacteria simply and effectively such as the E.coil O157:H7.

Description

technical field [0001] The invention relates to a nano biological separator, more specifically, the invention relates to an immunomagnetic nano particle bacteria separator. The invention also relates to the preparation method and application of the separator. Background technique [0002] As we all know, the field of nanotechnology is the most popular field of science and technology research today. The application of nanotechnology in the field of biological sciences forms an emerging science and technology-nanobiotechnology, which uses nanotechnology to study and solve major problems in the field of life sciences. Science, it is becoming one of the current important frontier scientific research fields. [0003] Nowadays, the application prospect of magnetic nanoparticles in the field of biotechnology has attracted increasing attention. Magnetic nanoparticles are often used as separation media for magnetic field separation of biological samples. The separation method usin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20G01N33/53C12Q1/04
Inventor 沈鹤柏陈伟严亚贤朱向玲
Owner SHANGHAI NORMAL UNIVERSITY
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