The invention provides a herbicide combination, containing Trifloxysulfuron, Pyrithiobac-sodium, APP and/or Cyclohexenone, and the ratio of parts by weights of Trifloxysulfuron: Pyrithiobac-sodium:APP and/or Cyclohexenone is 0.1:1:20-20:1:0.1. The combination can be used to prepare emulsifiable solution, liquor, thick emulsion, microemulsion, wettable powder, water suspending agent, oil suspending agent, water-dispersible granules or soluble powder (granules). The herbicide combination can be used to prevent and control Gramineae and broad-leaved weeds which have grown for one year or more than one year in the cotton field.