The invention discloses a special Chinese honey locust liquid soap used in a clinical laboratory and the preparation method thereof. The liquid soap comprises functional components, organic acid, a hard water resisting agent, a pure plant spice, a thickening agent and deionized water. The functional components are a Chinese honey locust extractive, a plant acid liquid and aloe-emodin; the organic acid adopts citric acid, Chinese honey locust has a bacterial inhibition effect, the saponin component contained in the Chinese honey locust can lower surface stress, the foaming is long lasting, the cleaning effect is good, the plant acid liquid is acidic, has a broad-spectrum bactericidal effect, and has a remarkable antiinflammation and antipruritic effects, and the citric acid is used for preparing a buffering solution, is taken as a builder, can improve the performance of washed products, and can improve the performance of a surface active agent. According the special Chinese honey locust liquid soap, the formula is reasonable, the preparation process is simple, the cost is low, the smell is fragrant, the sterilizing and disinfecting effects are obvious, a skin care effect is played, and the special Chinese honey locust liquid soap is specially provided for patients receiving examinations and staffs in the clinical laboratory, can eliminate influences from various germs and further improve the examining accuracy.