The invention discloses a production technology of an anti-static fabric. An electric conduction wire and a polyester cotton yarn are double-twisted to obtain a conductive yarn, the conductive yarn iswoven at intervals to obtain an anti-static gray fabric, and through singeing, shape setting and dyeing, the dyed gray fabric is obtained; through anti-static after-finishing of an anti-static finishing solution, two-time soaking and two-time rolling, the anti-static gray fabric is obtained; finally, through antibacterial anti-mite finishing of an antibacterial anti-mite finishing agent, two-timesoaking and two-time rolling, the anti-static fabric is obtained. The prepared anti-static fabric has good anti-static performance, can achieve moisture absorption and air permeation, has antibacterial and anti-mite performance and is good in comfort and particularly suitable for being used as a fabric of outdoor sports wear and climbing wear. The point-to-point resistance of the anti-static fabric reaches 2.8*1,010 omega, the rate of resisting staphylococcus aureus reaches 89.7%, the rate of resisting escherichia coli reaches 86.8%, the rate of resisting candida albicans reaches 73.6%, the water absorption rate reaches 106%, and the amount of water vapor transmission reaches 8,125 g/m<2>.24 h.