The invention relates to weeding composition containing diflufenican, which comprises an active ingredient A, an active ingredient B, auxiliary agent and other excipients. The active ingredient A is diflufenican, and the active ingredient B is selected from one of the following compounds: fluoroglycofen-ethyl, dicamba or metolachlor. The weeding composition is mainly characterized in that: the weight percentage of the active ingredient A is 1 percent to 60 percent, and the weight percentage of active ingredient B is 1 percent to 80 percent; and the weeding composition can be made into wettable powder, water dispersion granule, suspending agent, suspension emulsion, water emulsion and micro emulsion, can be used for preventing weeds in soybean field, wheat field and corn field, and has the characteristics of less use of farm chemicals, less residue of farm chemical, good environment compatibility and safety to the human beings and animals.