The invention discloses a medical sharp instrument-caused wound cleaning agent specially for an operating room and a preparation method thereof. The medical sharp instrument-caused wound cleaning agent is prepared from Chinese lobelia, herba portulacae, fresh dandelion, herba violae, paris polyphylla, garlic, rheum officinale, Chinese gall, phellodendron, cordate houttuynia, radix scutellariae, fructus forsythia, folium isatidis, isatis root, wild chrysanthemum flower and honeysuckle flower. The medical sharp instrument-caused wound cleaning agent has effects of sterilization and disinfection and can effectively inhibit viruses. Through use of a trace amount of western medicines such as interferon, fluvastatin, zidovudine and interleukin-2, the medical sharp instrument-caused wound cleaning agent can inhibit virus copying and is used for emergency pre-treatment on wounds incipiently suffering from hepatitis B virus, hepatitis C virus or HIV viruses. The medical sharp instrument-caused wound cleaning agent is mainly used for treating sharp instrument-caused wounds by a doctor and a nurse in a medical operation, is prepared by simple processes, is stored easily, realizes effective pre-treatment on wounds, has substantial effects of killing bacteria and inhibiting viruses, is safe, has no side effects and greatly protects the doctor and nurse.