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Metal chelated nanometer magnetic beads and preparation method and application therefor

A nano-magnetic bead and metal chelation technology, applied in biochemical equipment and methods, inorganic material magnetism, material inspection products, etc., can solve the problems of unsuitable proteomics and single protein types, and achieve simple and simplified separation process The effect of processing steps and easy operation

Inactive Publication Date: 2007-08-01
BIOMEDICAL ANALYSIS CENT OF ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing magnetic beads for protein extraction are mainly immunomagnetic beads, which bind to the corresponding protein through specific antigens or antibodies on the surface of the magnetic beads, but this method extracts a single type of protein, which is not suitable for the application of proteomics

Method used

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  • Metal chelated nanometer magnetic beads and preparation method and application therefor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of Nano Fe3O4 Particles of Embodiment 1 Superparamagnetic Surface Coating Hydroxyl

[0029] 1. Materials:

[0030] FeCl3: Analytical pure AR, batch number QD, March 11, 2004, produced by Sinopharm Chemical Reagent Co., Ltd.; PEG 1500: Mr1400-1600, batch number: 25300-68-3, FLUKA product; pyrrolidone (2-pyrrolidone): for synthesis, S34844146, product of MERCK-Schuchardt; diethyl ether: analytically pure, batch number: 20050315, produced by Tianjin Chemical Reagent Co., Ltd.; acetone, analytically pure, batch number: 20041017, produced by Beijing Chemical Plant; transmission electron microscope Philips CM120; Bio-Rad company FTS-65A Fu Lie transform infrared spectrometer.

[0031] 2. Method results:

[0032] A one-step synthesis of nano-magnetic beads with hydroxyl cores of Fe3O4 attached to the surface by thermal hydrolysis.

[0033] 1.35gFeCl 3 Fully dissolve in excess pyrrolidone, and add polyethylene glycol PEG-1500 7.5g under stirring condition, PEG-1...

Embodiment 2

[0034] Example 2 Preparation of Magnetic Beads with Surface Chelated Metal Copper

[0035] 1. Materials

[0036] Sodium hydroxide: analytically pure, batch number 20041112 Beijing Chemical Plant; epichlorohydrin: analytically pure, batch number 981219, Tianjin Chemical Reagent No. 6 Factory; dimethyl sulfoxide: 99.7%, batch number A0208798001, ACROS ORGANICS product; iminodiacetic acid (IDA): chemically pure, lot number F20041203, produced by Sinopharm Chemical Reagent Co., Ltd.; copper sulfate: analytically pure, lot number 950315, produced by Beijing Chemical Plant; nitric acid: analytically pure, lot number 20001110, produced by Beijing Lisui Chemical Plant; hydrogen peroxide: Analytical grade, batch number 20040124, Tianjin Dongfang Chemical Factory, ICP-MS 7500 Agilent Technologies Co., Ltd.; transmission electron microscope Philips CM120.

[0037] 2. Method results

[0038] (1) Preparation of magnetic beads with surface chelated metal copper

[0039] ①In the above-men...

Embodiment 3

[0046] The application of embodiment three metal copper chelated nano-magnetic beads

[0047] 1. Materials:

[0048] Binding buffer solution: 0.1mol / l phosphate buffer solution, pH 4; disodium hydrogen phosphate, analytical grade, batch number 020422, Beijing Chemical Reagent Company; sodium dihydrogen phosphate, analytical grade, batch number 000404, Beijing Chemical Reagent Company; washing buffer Solution: 0.1% formic acid aqueous solution, formic acid: analytically pure, batch number 000815, Beijing Chemical Reagent Company; eluent: 0.1% formic acid and 50% acetonitrile aqueous solution, acetonitrile, chromatographically pure, batch number 401103135 Handan Linfeng Fine Chemical Co., Ltd.; Serum samples were obtained from PLA General Hospital.

[0049] 2. Method results:

[0050] Take 20 μL of the prepared metal copper chelated nano-magnetic beads, wash 3 times with the binding buffer solution, make the magnetic beads under the condition of the binding buffer solution, se...

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Abstract

This invention discloses one metal chelate nanometer magnetic balls and its process method and application, which comprises the following steps: using metal salt and surface to cover hydroxyl group nanometer magnetic ball as main materials to complex metal ion to get the magnetic ball; then using body liquid to extract protein and polypeptide by use of magnetic field; the protein polypeptide for basic aid laser to absorb fly time mass spectrum and electrical spray mass spectrum for analysis.

Description

technical field [0001] The invention relates to a magnetic nano particle, in particular to a metal chelating nano magnetic bead, and also to a preparation method and application of the magnetic bead. Background technique [0002] Proteins are the main executors of various metabolic and regulatory pathways that biological cells rely on for survival, and the occurrence of diseases may be related to changes in protein modification status. Proteomics research is directly located at the protein level, but it is different from previous research on protein chemistry. , the object of proteome research is not a single or a few proteins, it focuses on the study of gene functions from a comprehensive, holistic, dynamic, and quantitative perspective, and uses proteome research methods to gain a panoramic perspective from the overall level of cellular protein expression Changes in cell protein expression will be more helpful to understand the biological characteristics of cells, so the u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C12Q1/00H01F1/01
Inventor 张学敏王娜王红霞李萍董芳霆李爱玲王杰
Owner BIOMEDICAL ANALYSIS CENT OF ACADEMY OF MILITARY MEDICAL SCI
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