Method for separating and purifying L-ornithine by using simulated moving bed

A technology of simulating moving bed and ornithine, applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., can solve the problem of L-ornithine fermentation broth with many impurities and low L-ornithine yield , large consumption of water and eluent, etc., to achieve the effect of eliminating acid and alkali consumption, reducing extraction cost and improving yield
CN101774935AActive Publication Date: 2010-07-14NANJING UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING UNIV OF TECH
Publication Date
2010-07-14

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Abstract

The invention belongs to the field of biological pharmaceutics, and discloses a method for separating and purifying L-ornithine by using a simulated moving bed. The method comprises the following steps: firstly, removing bacteria and solid substances from L-ornithine fermentation solution by using ultra-filtration; secondly, feeding the solution into the simulated moving bed, adsorbing the solution, washing the impurities, eluting the L-ornithine, collecting the elution and regenerating a column; and finally, concentrating and decolorizing the collected elution, adjusting the pH of the elution to between 4.5 and 5.0 with hydrochloric acid, crystallizing the elution, and drying the crystal to obtain L-ornithine hydrochloride crystal. The L-ornithine separated and purified by using the method has the advantages of high yield, high purity, low cost, environmental protection, and suitability for industrialized production.
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Description

technical field

[0001] The invention belongs to the field of biopharmaceuticals, and relates to a method for separating and purifying L-ornithine from fermentation liquid by using a simulated moving bed. Background technique

[0002] L-ornithine is an important non-protein constituent amino acid and a precursor for the biosynthesis of L-arginine. L-ornithine plays an extraordinary role in the human body, the formation of urea must have the participation of L-ornithine, the lack of L-ornithine may bring danger to life. Now L-ornithine is widely used in medicine and food industry, mainly used in the treatment of acute and chronic hepatitis, hepatic encephalopathy and other diseases (GB1020492, 1966), the recovery of the patient's body, sports drinks, and the prevention and treatment of benign cysts and malignant tumors (CA2404275, 2001) and so on.

[0003] At present, domestic and foreign reports on the preparation methods of L-ornithine mainly include chemical methods, enzy...

Claims

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