Method for separating and purifying L-ornithine by using simulated moving bed

A technology of simulating moving bed and ornithine, applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., can solve the problem of L-ornithine fermentation broth with many impurities and low L-ornithine yield , large consumption of water and eluent, etc., to achieve the effect of eliminating acid and alkali consumption, reducing extraction cost and improving yield

Active Publication Date: 2010-07-14
NANJING UNIV OF TECH
View PDF4 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method is mainly to synthesize DL-ornithine by alkaline hydrolysis of L-arginine and acrylic acid and sodium cyanide. In addition, the chemical method requires a large amount of organic solvents, which seriously pollutes the environment; the enzymatic conversion method uses L-arginine as raw material, ferments Cultivate cells and catalyze the conversion into L-ornithine by the enzymes produced; the production of L-ornithine by microbial fermentation mainly uses low-cost raw materials for submerged liquid fermentation to directly produce L-ornithine. The fermentation level is relatively lower than that of foreign countr

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating and purifying L-ornithine by using simulated moving bed
  • Method for separating and purifying L-ornithine by using simulated moving bed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The above-mentioned L-ornithine fermentation liquid first uses the ultrafiltration method (150,000 Dalton titanium dioxide ceramic membrane ultrafiltration, the ultrafiltration operating pressure is 0.38MPa, and the operating temperature is 55°C) to remove bacteria and solids to obtain ultrafiltration. Liquid (ie feed liquid, the same below);

[0045] combine figure 1 , the simulated moving bed system is sequentially divided into four areas: adsorption area, washing area, elution area and regeneration washing area; the feed liquid inlet and raffinate outlet are located in the adsorption area, and the washing agent inlet and washing liquid outlet are located in the washing area. Miscellaneous area, the eluent inlet and extractant (i.e. eluent, the same below) outlet are located in the elution area, and the regenerant inlet and outlet are located in the regeneration area; the simulated moving bed system uses 14 columns, of which there are 6 columns in the adsorption area....

Embodiment 2

[0050] The above-mentioned L-ornithine fermentation broth is firstly removed by ultrafiltration (150,000 Dalton titanium dioxide ceramic membrane ultrafiltration, the ultrafiltration operating pressure is 0.38MPa, and the operating temperature is 58°C) to remove bacteria and solids to obtain ultrafiltration. Liquid (i.e. feed liquid);

[0051] combine figure 1 , the simulated moving bed system is sequentially divided into four areas: adsorption area, washing area, elution area and regeneration washing area; the feed liquid inlet and raffinate outlet are located in the adsorption area, and the washing agent inlet and washing liquid outlet are located in the washing area. Miscellaneous area, the eluent inlet and extract outlet are located in the elution area, the regenerant inlet and outlet are located in the regeneration area; the simulated moving bed system uses 14 columns, of which there are 6 columns in the adsorption area, 2 columns in the washing area, and 2 columns in the...

Embodiment 3

[0056] The above-mentioned L-ornithine fermentation broth is firstly removed by ultrafiltration (50,000 Dalton titanium dioxide ceramic membrane ultrafiltration, the ultrafiltration operating pressure is 0.35MPa, and the operating temperature is 60°C) to remove bacteria and solids to obtain ultrafiltration. Liquid (i.e. feed liquid);

[0057] combine figure 1 , the simulated moving bed system is sequentially divided into four areas: adsorption area, washing area, elution area and regeneration washing area; the feed liquid inlet and raffinate outlet are located in the adsorption area, and the washing agent inlet and washing liquid outlet are located in the washing area. Miscellaneous area, the eluent inlet and extract outlet are located in the elution area, the regenerant inlet and outlet are located in the regeneration area; the simulated moving bed system uses 14 columns, of which there are 6 columns in the adsorption area, 2 columns in the washing area, and 2 columns in the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention belongs to the field of biological pharmaceutics, and discloses a method for separating and purifying L-ornithine by using a simulated moving bed. The method comprises the following steps: firstly, removing bacteria and solid substances from L-ornithine fermentation solution by using ultra-filtration; secondly, feeding the solution into the simulated moving bed, adsorbing the solution, washing the impurities, eluting the L-ornithine, collecting the elution and regenerating a column; and finally, concentrating and decolorizing the collected elution, adjusting the pH of the elution to between 4.5 and 5.0 with hydrochloric acid, crystallizing the elution, and drying the crystal to obtain L-ornithine hydrochloride crystal. The L-ornithine separated and purified by using the method has the advantages of high yield, high purity, low cost, environmental protection, and suitability for industrialized production.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to a method for separating and purifying L-ornithine from fermentation liquid by using a simulated moving bed. Background technique [0002] L-ornithine is an important non-protein constituent amino acid and a precursor for the biosynthesis of L-arginine. L-ornithine plays an extraordinary role in the human body, the formation of urea must have the participation of L-ornithine, the lack of L-ornithine may bring danger to life. Now L-ornithine is widely used in medicine and food industry, mainly used in the treatment of acute and chronic hepatitis, hepatic encephalopathy and other diseases (GB1020492, 1966), the recovery of the patient's body, sports drinks, and the prevention and treatment of benign cysts and malignant tumors (CA2404275, 2001) and so on. [0003] At present, domestic and foreign reports on the preparation methods of L-ornithine mainly include chemical methods, enzy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07C229/26C07C227/40B01D15/36B01D15/04
Inventor 万红贵周华蔡恒朱庆平袁建锋
Owner NANJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap