Preparation of nano gel immobilized multienzyme system and application thereof in synthesizing 1,3-propylene glycol

A technology of nanogel and multi-enzyme system, applied in the direction of immobilized on or in the inorganic carrier, multi-enzyme system, fermentation, etc., can solve problems such as conformational changes and molecular movement restrictions, enzyme activity reduction, and damage to the microenvironment. Achieve the effect of reducing the influence of diffusion, inhibiting agglomeration, and simple reaction
CN101914520AInactive Publication Date: 2010-12-15SOUTHEAST UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTHEAST UNIV
Publication Date
2010-12-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to preparation of a nano gel immobilized multienzyme system. The preparation method comprises the following steps: constructing a nano gel-multienzyme assembling system by using a nano gel dispersion-adsorption immobilized enzyme technology, carrying out self-coupling, and catalyzing by multiple enzymes to synthesize the 1,3-propylene glycol. The nano gel suspension, which can be evenly dispersed in the water solution, is prepared to directly absorb the immobilized multienzyme system. The method can effectively inhibit the aggregation and enhance the dispersibility; the invention fully displays the excellent properties of nano gel carrier, such as high specific surface and the like, provides a location for simultaneously coupling multiple biological enzymes (multienzyme immobilization), and reduces the influence of the carrier on the dispersion of a substrate and products; and compared with the microbe fermentation method, the invention has the advantages of low cost, simple reaction, no cell pollution and the like, and the nano gel immobilized multienzyme system can not be easily degraded by the microbes. The invention provides a new effective catalytic method for synthesizing nano gel immobilized enzymes.
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Description

technical field

[0001] The present invention relates to the preparation of a nano-gel immobilized multi-enzyme system and its application in the synthesis of 1,3-propanediol (1,3-PD). By preparing a nano-gel suspension uniformly dispersed in an aqueous solution, using Dispersion-adsorption immobilization technology, construction of nanogel-multi-enzyme assembly system, multi-enzyme coupling catalytic synthesis of 1,3-PD. This method can effectively inhibit agglomeration, improve dispersibility, form a uniform and stable nanogel suspension, and exert the excellent properties of nanocarriers such as high specific surface area. Diffusion effects of small carriers on substrates and products. The use of nanogel immobilized multi-enzyme system to catalyze the synthesis of 1,3-PD has the advantages of low cost, simple reaction, no cell pollution, and not easy to be degraded by microorganisms compared with microbial fermentation methods. The invention belongs to the technical field ...

Claims

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