Method for simultaneous extraction of a plurality of target substances with different polarity in sample

A target and sample technology, which is applied in the preparation of test samples, chemical instruments and methods, chemical/physical processes, etc., can solve the problems of inability to extract the target at the same time, missed detection in the pretreatment method, and high cost, and achieve low cost. , improve the accuracy, and the effect of efficient extraction

Active Publication Date: 2012-12-26
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0016]Technical problem: the purpose of the present invention is to overcome the existing sample pretreatment technology can only "one-to-one" extract a certain type of compound with similar properties, that is, different properties The target objects must be extracted by different methods, and it is impossible to simultaneously extract a variety of target objects with different chemical properties from polar to non-polar in a "one-to-many" manner, which is time-consuming, laborious, and costly, and causes Some targets, especially polar targets, have defects such as "missing detection" due to lack of suitable pretreatment methods and ineffective extraction. A functionalized polypyrrole nanofiber membrane is proposed, which can realize multiple properties at the same time. Sample pretreatment method for high-throughput "one-to-many" efficient extraction of different targets

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Sample: environmental water sample

[0034] Target substances: DDT, butylbenzyl phthalate, dibutyl phthalate, bis(2-ethyl)hexyl phthalate, bisphenol A.

[0035] With nylon 6 as raw material, formic acid as solvent, the mass concentration of nylon 6 is 25%, add 10% pyrrole monomer, and magnetically stir to obtain the electrospinning solution. During the electrospinning process, 15% ferric chloride solution is continuously added dropwise to the Collect the plate to prepare the nanofiber base film; apply a voltage for 1 hour by cyclic voltammetry, and deposit a PPy coating with a thickness of 30 μm doped with dodecyl sulfate as the counter ion on the nanofiber base film to obtain a functional PPy nanofiber membrane.

[0036] Weigh 3.5 mg of functionalized PPy nanofiber membrane and put it into a membrane disk solid phase extraction apparatus, activate and wash with acetone → water → acetone → water (200 μl each); after filtering 50 mL of environmental water samples, take ...

Embodiment 2

[0038] Sample: freshwater fish

[0039] Target substances: estradiol, ethinyl estradiol, estrone, diethylstilbestrol, atrazine, simazine, dimethoate.

[0040] With PLGA as raw material, dichloromethane as solvent, the mass concentration of PLGA is 12%, add 7% pyrrole monomer, and magnetically stir to obtain the electrospinning solution, continuously drop 5% ferric chloride solution in the electrospinning process Collect the plate to make a nanofiber basement membrane; apply a voltage for 1 hour by cyclic voltammetry, and deposit a PPy coating with a thickness of 40 μm on the nanofiber basement membrane that is mixed with polystyrene sulfonate ions as counter ions to obtain a functional PPy nanofiber membrane.

[0041] Weigh 5.0 mg of functionalized PPy nanofiber membrane and put it into a membrane disc solid phase extraction apparatus, activate and wash with acetonitrile → water → acetonitrile → water (200 μl each); The target substance was retained on the membrane by passin...

Embodiment 3

[0043] Sample: Farmland Soil

[0044] Target substances: polycyclic aromatic hydrocarbons, cypermethrin, deltamethrin

[0045] Using polystyrene as raw material, N,N-dimethylformamide / tetrahydrofuran (40 / 60) as solvent, the mass concentration of polystyrene is 102%, adding 10% pyrrole monomer, and magnetic stirring to obtain electrospinning solution During the electrospinning process, 6% ferric chloride solution was continuously added dropwise to the collecting plate to obtain the nanofiber basement membrane; the voltage was applied by the constant voltage method for 2 hours, and the nanofiber basement membrane with a thickness of 80 μm was deposited on the nanofiber basement membrane. The polystyrene sulfonate ion is the PPy coating of the ion, and the functionalized PPy nanofiber membrane is obtained.

[0046] Weigh 5.0mg of functionalized PPy nanofiber membrane and put it into the membrane disc solid phase extraction apparatus, activate and wash with n-hexane→water→n-hexan...

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Abstract

The invention brings forward a method for simultaneous extraction of a plurality of target substances with different polarity in a sample based on a functionalized polypyrrole (PPy) nanometer fiber film. According to the invention, a sample to be detected is prepared into a sample solution with an aqueous matrix after preliminary treatment; with the help of extraction disks for solid phase extraction, the sample solution is allowed to pass through the activated functionalized PPy nanometer fiber film, and a plurality of target substances with different polarity in the sample undergo interactions of different properties with the functionalized PPy nanometer fiber film and are retained simultaneously; target substances with nonpolarity, weak polarity, middle polarity and polarity are respectively eluted from the nanometer fiber film step by step by using eluants of different intensity and are analyzed and detected. The method provided in the invention overcomes the defects that the existing sample pretreatment technology can only used for one to one extraction of a certain kind of compounds of similar properties, that target substances with different properties must be extracted by using different methods, that the existing sample pretreatment technology is time consuming and labor consuming and costs a lot and that polar target substances in a sample to be detected is prone to be skipped for detection, and has a wide application prospect.

Description

technical field [0001] The invention provides a method based on the polypyrrole functionalized nanofiber membrane, which can simultaneously extract multiple target objects with different polarities in a sample. The invention belongs to the technical field of sample pretreatment in analytical chemistry. Background technique [0002] Sample pretreatment is the bottleneck problem in the development of modern analytical chemistry. Solid Phase Extraction (Solid Phase Extraction, SPE), which has the advantages of less solvent usage, simple and fast operation, high selectivity, good reproducibility, and easy automation, is in line with the development trend of sample processing technology and is a sample processing technology. research hotspots in the field. [0003] Materials are the fundamental prerequisite for efficient extraction by SPE. Material molecules are the most basic structural units that embody the functions of materials, and the diversity of molecular structures d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/34G01N1/40B01D15/08B01J20/286B01J20/30
Inventor 许茜
Owner SOUTHEAST UNIV
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